AB137763

Anti-TRAF5 antibody

Name: Anti-TRAF5 antibody (ab137763) | Abcam
Brand: Abcam Limited
SKU: AB137763
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal TRAF5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRAF5 aa 1-200.

View Alternative Names

RNF84, TRAF5, TNF receptor-associated factor 5, RING finger protein 84

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-TRAF5 antibody (AB137763)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRAF5 antibody (AB137763)

Immunofluorescence analysis of methanol fixed HeLa labeling TRAF5 with ab137763 at a 1/200 dilution.
The lower image was costained with Hoechst 33342.

Unknown

Western blot - Anti-TRAF5 antibody (AB137763)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TRAF5 antibody (ab137763) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 64 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TRAF5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O00463

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF5 known as TNF receptor-associated factor 5 plays a role in cellular signaling pathways. This protein has a molecular mass of approximately 64 kDa. It is expressed in various tissues including lymphoid tissues and cells of the immune system. TRAF5 acts in signaling downstream of certain receptors like TNF receptor superfamily where it functions as an adapter protein that mediates signal transduction by interacting with other signaling molecules.

Biological function summary

TRAF5 influences immune cell functions and inflammatory responses. It forms part of a larger protein complex that regulates the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. Through this complex TRAF5 affects the transcription of genes involved in immune responses and cell survival. By modulating these processes TRAF5 helps maintain immune homeostasis and ensures proper immune response.

Pathways

TRAF5 is involved in signaling pathways such as NF-κB and JNK (c-Jun N-terminal kinase) pathways. TRAF5 interacts with proteins like TRAF2 and RIPK1 to propagate signals that lead to activation of these pathways. NF-κB pathway regulates inflammation and immune responses while JNK pathway plays a part in stress responses and apoptosis. The interaction of TRAF5 with these proteins and pathways positions it as an integral part of the immune signaling network.

TRAF5 associates with autoimmune diseases including rheumatoid arthritis and lupus. Abnormal signaling through TRAF5 can lead to dysregulated immune responses contributing to these conditions. In rheumatoid arthritis for example TRAF5 may abnormally activate NF-κB pathway leading to enhanced inflammation. Moreover the interaction between TRAF5 and other proteins like TRAF1 influences the development of these diseases by affecting signal transduction which is important for understanding potential therapeutic targets in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.

See full target information TRAF5

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 29:206-217 PubMed34465890

2021

Mechanosensitive turnover of phosphoribosyl pyrophosphate synthetases regulates nucleotide metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Jichun Shao,Zhijun Zeng,Yumin He,Can Tang,Su Hwan Park,Jong-Ho Lee,Rui Liu

Journal of biochemical and molecular toxicology 35:e22867 PubMed34369042

2021

Downregulation of long noncoding RNA SNHG7 protects against inflammation and apoptosis in Parkinson's disease model by targeting the miR-425-5p/TRAF5/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiquan Zhang,Zhiyong Wang,Keqi Hu,Handong Liu

The journal of gene medicine 22:e3134 PubMed31656043

2020

LINC00467 promotes cell proliferation and metastasis by binding with IGF2BP3 to enhance the mRNA stability of TRAF5 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjin Jiang,Xueling Cheng,Tao Wang,Xuepeng Song,Yanbo Zheng,Ligang Wang

Biochemical and biophysical research communication 514:699-705 PubMed31078266

2019

MiR-141-3p inhibits cell proliferation, migration and invasion by targeting TRAF5 in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Liang,Xiangle Li,Shaoqiong Liu,Chunhua Li,Xiaolan Wang,Jiaotao Xing

Cellular & molecular biology letters 24:27 PubMed31011336

2019

Upregulation of miR-29b-3p protects cardiomyocytes from hypoxia-induced apoptosis by targeting TRAF5.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Cai,Yunpeng Li

International journal of molecular medicine 41:555-563 PubMed29115406

2017

Berberine prevents the apoptosis of mouse podocytes induced by TRAF5 overexpression by suppressing NF-κB activation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wu,Dong-Sheng Yao,Tian-Ying Lan,Chen Wang,Jian-Dong Gao,Li-Qun He,Di Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAF5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TRAF5 antibody (AB137763)

Western blot - Anti-TRAF5 antibody (AB137763)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise