Rabbit Polyclonal TRAF6 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRAF6 aa 100-450.
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- Journal of nanobiotechnology 21:4192023Exosomal miR-146a-5p derived from human umbilical cord mesenchymal stem cells can alleviate antiphospholipid antibody-induced trophoblast injury and placental dysfunction by regulating the TRAF6/NF-κB axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingfeng Lv,Yuan Wang,Wei Tian,Yuqiu Liu,Mengqi Gu,Xiaotong Jiang,Yanjun Cai,Ruiheng Huo,Yuchen Li,Lei Li,Xietong WangPubMed 37957714
- Chinese medicine 18:1432023Xinyang tablet ameliorates sepsis-induced myocardial dysfunction by regulating Beclin-1 to mediate macrophage autophagy and M2 polarization through LncSICRNT1 targeting E3 ubiquitin ligase TRAF6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Luo,Yuanmei Li,Liwei He,Haitao Tu,Xinfeng Lin,Fengli Zhao,Yusheng Huang,Minyong Wen,Lingjun Wang,Zhongqi YangPubMed 37919806
- Journal of musculoskeletal & neuronal interactions 22:536-5452022USP1 Inhibits NF-κB/NLRP3 Induced Pyroptosis through TRAF6 in Osteoblastic MC3T3-E1 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDengshuo Sun,Yi Peng,Shengyang Ge,Qiang FuPubMed 36458391
- Journal of inflammation research 15:4853-48722022Alpha-Momorcharin Inhibits Proinflammatory Cytokine Expression by M1 Macrophages but Not Anti-Inflammatory Cytokine Expression by M2 Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKejun Peng,Nianhua Deng,Yao Meng,Qianchuan He,Hao Meng,Ting Luo,Yanru Wei,Yue Kang,Xiaodong Zhou,Fubing ShenPubMed 36042868
- Journal of microbiology and biotechnology 32:1344-13542022KIF26B-AS1 Regulates TLR4 and Activates the TLR4 Signaling Pathway to Promote Malignant Progression of Laryngeal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Li,Jiahui Han,Shujia Zhang,Chunguang Dong,Xiang XiaoPubMed 36224753
- Nature communications 13:28992022Anoctamin 1 controls bone resorption by coupling Cl channel activation with RANKL-RANK signaling transduction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeijia Sun,Shuai Guo,Yuheng Li,JianWei Li,Caizhi Liu,Yafei Chen,Xuzhao Wang,Yingjun Tan,Hua Tian,Cheng Wang,Ruikai Du,Guohui Zhong,Sai Shi,Biao Ma,Chang Qu,Jingxuan Fu,Xiaoyan Jin,Dingsheng Zhao,Yong Zhan,Shukuan Ling,Hailong An,Yingxian LiPubMed 35610255
- Open medicine (Warsaw, Poland) 17:907-9142022MicroRNA 322-5p reduced neuronal inflammation via the TLR4/TRAF6/NF-κB axis in a rat epilepsy model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Zhou,Qiong Wang,Baomei He,Haibo Kong,Huanjun Luo,Xiaowei Wang,Wenlan WangPubMed 35647304
- Frontiers in immunology 13:8626282022PE_PGRS38 Interaction With HAUSP Downregulates Antimycobacterial Host Defense TRAF6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJae-Sung Kim,Hyo Keun Kim,Euni Cho,Seok-Jun Mun,Sein Jang,Jichan Jang,Chul-Su YangPubMed 35572598
- Journal of inflammation research 15:2213-22282022HSP70 Ameliorates Septic Acute Kidney Injury via Binding with TRAF6 to Inhibit of Inflammation-Mediated Apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiqiu Zhang,Chenlu Song,Wei Ni,Qing Pei,Caixia Wang,Youguo Ying,Min YaoPubMed 35411167
- Frontiers in cellular and infection microbiology 12:7590772022ASB17 Facilitates the Burst of LPS-Induced Inflammation Through Maintaining TRAF6 Stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPin Wan,Ge Yang,Simeng Zhang,Yaru Zhang,Yaling Jia,Xu Che,Zhen Luo,Pan Pan,Geng Li,Xulin Chen,Qiwei Zhang,Wen Zhang,Qiuping Tan,Yongkui Li,Jianguo WuPubMed 35174103
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TRAF6 aa 100-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4K3
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Mouse | Expected | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as ECSIT, IKBKG, IRAK1, AKT1 and AKT2 (PubMed:11057907, PubMed:18347055, PubMed:19465916, PubMed:19713527, PubMed:31620128). Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation (PubMed:19675569). Leads to the activation of NF-kappa-B and JUN (PubMed:16378096, PubMed:17135271, PubMed:17703191). Seems to also play a role in dendritic cells (DCs) maturation and/or activation (By similarity). Represses c-Myb-mediated transactivation, in B-lymphocytes (PubMed:18093978, PubMed:18758450). Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor (PubMed:12140561, PubMed:19825828, PubMed:8837778). Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation (By similarity). Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production (By similarity). Participates also in the TCR signaling by ubiquitinating LAT (PubMed:23514740, PubMed:25907557).
Alternative names
RNF85, TRAF6, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6
Recommended products
Rabbit Polyclonal TRAF6 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRAF6 aa 100-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TRAF6 aa 100-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4K3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAF6 also known as TNF receptor-associated factor 6 is an E3 ubiquitin ligase that is part of the TRAF family. It has a molecular mass of approximately 60 kDa. TRAF6 is ubiquitously expressed in many types of tissues and cells including immune cells like macrophages and dendritic cells. Its primary role is to mediate signal transduction from members of the TNF receptor and Toll-like receptor (TLR) families which it achieves through polyubiquitination of target proteins. This process facilitates the recruitment of downstream signaling components necessary for cellular responses.
- Biological function summary
TRAF6 plays a critical role in regulating immune and inflammatory responses. It acts within a complex often associating with other signaling proteins such as IRAK1 and TAK1 to activate downstream kinases like IKK and JNK. These signaling pathways lead to the activation of transcription factors such as NF-kB and AP-1 which regulate the expression of genes involved in inflammation and immune responses. TRAF6 is an important modulator of adaptive and innate immunity influencing both the maturation of immune cells and the body's response to infection.
- Pathways
TRAF6 significantly contributes to the NF-kB and MAPK signaling pathways. In the NF-kB pathway TRAF6 mediates signal transduction following TLR engagement interacting with proteins such as MyD88 and RIP1 to drive inflammatory cytokine production. In the MAPK pathway it associates predominantly with TAK1 to facilitate the phosphorylation and activation of downstream kinases. These pathways are essential for transmitting signals from cell surface receptors to the nucleus controlling the magnitude of immune signaling and cellular stress responses.
- Associated diseases and disorders
TRAF6 has a significant association with autoimmune diseases and certain cancers. In autoimmune disorders aberrant activation of TRAF6 can lead to prolonged inflammation and detrimental immune responses. For cancers TRAF6 is linked to tumor progression and metastasis acting alongside proteins like IRAK1 and RIP1 which can further promote malignancy through chronic inflammation and immune evasion. Understanding TRAF6's role in these diseases provides insights that could lead to targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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9 product images
Western blot - Anti-TRAF6 antibody (ab137452)
10% SDS-PAGE
All lanes: Western blot - Anti-TRAF6 antibody (ab137452) at 1/2000 dilution
All lanes: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 55 kDa, 60 kDa
Observed band size: 56 kDa
Immunoprecipitation - Anti-TRAF6 antibody (ab137452)
IP samples: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate.
1- Control with 4 μg of preimmune Rabbit IgG;
2- Immunoprecipitation of TRAF6 protein by 4 μg ab137452.7.5 % SDS-PAGE
The immunoprecipitated TRAF6 protein was detected by ab137452 at 1/500 dilution, followed by anti-rabbit IgG antibody.
All lanes: Immunoprecipitation - Anti-TRAF6 antibody (ab137452)
Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (ab137452)
Paraffin-embedded 59T xenograft tissue stained TRAF6 using ab137452 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Immunocytochemistry/ Immunofluorescence - Anti-TRAF6 antibody (ab137452)
Immunofluorescence analysis of 4% paraformaldehyde-fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling TRAF6 at cytoplasm and nucleus (green) with ab137452 at 1/100 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution. Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (ab137452)
Paraffin-embedded mouse lung tissue stained TRAF6 at cytoplasm using ab137452 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western blot - Anti-TRAF6 antibody (ab137452)
7.5% SDS PAGE
All lanes: Western blot - Anti-TRAF6 antibody (ab137452) at 1/1000 dilution
Lane 1: A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (ab137452)
Paraffin-embedded rat brain tissue stained TRAF6 at cytoplasm using ab137452 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western blot - Anti-TRAF6 antibody (ab137452)
7.5% SDS PAGE
All lanes: Western blot - Anti-TRAF6 antibody (ab137452) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Lane 2: BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa
Western blot - Anti-TRAF6 antibody (ab137452)
7.5% SDS-PAGE
All lanes: Western blot - Anti-TRAF6 antibody (ab137452) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa
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