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AB137452

Anti-TRAF6 antibody

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(31 Publications)

Rabbit Polyclonal TRAF6 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 31 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRAF6 aa 100-450.

View Alternative Names

RNF85, TRAF6, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-TRAF6 antibody (AB137452)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRAF6 antibody (AB137452)

Immunofluorescence analysis of 4% paraformaldehyde-fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling TRAF6 at cytoplasm and nucleus (green) with ab137452 at 1/100 dilution. Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution. Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (AB137452)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (AB137452)

Paraffin-embedded 59T xenograft tissue stained TRAF6 using ab137452 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Immunoprecipitation - Anti-TRAF6 antibody (AB137452)
  • IP

Supplier Data

Immunoprecipitation - Anti-TRAF6 antibody (AB137452)

IP samples : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate.

1- Control with 4 μg of preimmune Rabbit IgG;
2- Immunoprecipitation of TRAF6 protein by 4 μg ab137452.

7.5 % SDS-PAGE

The immunoprecipitated TRAF6 protein was detected by ab137452 at 1/500 dilution, followed by anti-rabbit IgG antibody.

All lanes:

Immunoprecipitation - Anti-TRAF6 antibody (ab137452)

Predicted band size: 60 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (AB137452)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (AB137452)

Paraffin-embedded rat brain tissue stained TRAF6 at cytoplasm using ab137452 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (AB137452)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody (AB137452)

Paraffin-embedded mouse lung tissue stained TRAF6 at cytoplasm using ab137452 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-TRAF6 antibody (AB137452)
  • WB

Unknown

Western blot - Anti-TRAF6 antibody (AB137452)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TRAF6 antibody (ab137452) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 60 kDa

false

Western blot - Anti-TRAF6 antibody (AB137452)
  • WB

Supplier Data

Western blot - Anti-TRAF6 antibody (AB137452)

10% SDS-PAGE

All lanes:

Western blot - Anti-TRAF6 antibody (ab137452) at 1/2000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 55 kDa,60 kDa

Observed band size: 56 kDa

false

Western blot - Anti-TRAF6 antibody (AB137452)
  • WB

Supplier Data

Western blot - Anti-TRAF6 antibody (AB137452)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-TRAF6 antibody (ab137452) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 60 kDa

false

Western blot - Anti-TRAF6 antibody (AB137452)
  • WB

Unknown

Western blot - Anti-TRAF6 antibody (AB137452)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TRAF6 antibody (ab137452) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 2:

BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TRAF6 aa 100-450. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4K3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF6 also known as TNF receptor-associated factor 6 is an E3 ubiquitin ligase that is part of the TRAF family. It has a molecular mass of approximately 60 kDa. TRAF6 is ubiquitously expressed in many types of tissues and cells including immune cells like macrophages and dendritic cells. Its primary role is to mediate signal transduction from members of the TNF receptor and Toll-like receptor (TLR) families which it achieves through polyubiquitination of target proteins. This process facilitates the recruitment of downstream signaling components necessary for cellular responses.
Biological function summary

TRAF6 plays a critical role in regulating immune and inflammatory responses. It acts within a complex often associating with other signaling proteins such as IRAK1 and TAK1 to activate downstream kinases like IKK and JNK. These signaling pathways lead to the activation of transcription factors such as NF-kB and AP-1 which regulate the expression of genes involved in inflammation and immune responses. TRAF6 is an important modulator of adaptive and innate immunity influencing both the maturation of immune cells and the body's response to infection.

Pathways

TRAF6 significantly contributes to the NF-kB and MAPK signaling pathways. In the NF-kB pathway TRAF6 mediates signal transduction following TLR engagement interacting with proteins such as MyD88 and RIP1 to drive inflammatory cytokine production. In the MAPK pathway it associates predominantly with TAK1 to facilitate the phosphorylation and activation of downstream kinases. These pathways are essential for transmitting signals from cell surface receptors to the nucleus controlling the magnitude of immune signaling and cellular stress responses.

TRAF6 has a significant association with autoimmune diseases and certain cancers. In autoimmune disorders aberrant activation of TRAF6 can lead to prolonged inflammation and detrimental immune responses. For cancers TRAF6 is linked to tumor progression and metastasis acting alongside proteins like IRAK1 and RIP1 which can further promote malignancy through chronic inflammation and immune evasion. Understanding TRAF6's role in these diseases provides insights that could lead to targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as ECSIT, IKBKG, IRAK1, AKT1 and AKT2 (PubMed : 11057907, PubMed : 18347055, PubMed : 19465916, PubMed : 19713527, PubMed : 27746020, PubMed : 31620128). Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation (PubMed : 19675569). Leads to the activation of NF-kappa-B and JUN (PubMed : 16378096, PubMed : 17135271, PubMed : 17703191). Seems to also play a role in dendritic cells (DCs) maturation and/or activation (By similarity). Represses c-Myb-mediated transactivation, in B-lymphocytes (PubMed : 18093978, PubMed : 18758450). Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor (PubMed : 12140561, PubMed : 19825828, PubMed : 8837778). Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation (By similarity). Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production (By similarity). Acts as a regulator of the JNK and NF-kappa-B signaling pathways by initiating assembly of heterotypic 'Lys-63'-/'Lys-48'-linked branched ubiquitin chains that are then recognized by TAB2 : TRAF6 catalyzes initial 'Lys-63'-linked-polyubiquitin chains that are then branched via 'Lys-48'-linked polyubiquitin by HUWE1 (PubMed : 27746020). 'Lys-63'-/'Lys-48'-linked branched ubiquitin chains protect 'Lys-63'-linkages from CYLD deubiquitination (PubMed : 27746020). Participates also in the TCR signaling by ubiquitinating LAT (PubMed : 23514740, PubMed : 25907557).
See full target information TRAF6
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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Cancer gene therapy 32:1206-1217 PubMed40855365

2025

Characterization of tertiary lymphoid structure identifies competitive binding of CD40 and STING with TRAF2 driving IRF4-mediated B cell activation in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yujia Zheng,Donglai Chen,Yi Xu,Xuejun Xu,Peidong Song,Xuejie Wu,Lijie Tan,Yiming Mao,Yongbing Chen

Inflammation : PubMed40447963

2025

XBP1 Knockdown Alleviates Pyroptosis and Promotes Th17/Treg Imbalance in Periodontitis by Inhibiting the IL-17 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lixun Kang,Binglu Shi,Siyu Shen,Kai Ma,Yuanxu Jing,Qi An,Yan Dai

British journal of haematology 206:1627-1641 PubMed40325612

2025

Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Yingbin Yue,Yunfei Zhang,Yongfeng Cheng,Hongjie Jiao,Mei Yan

Inflammation 48:3050-3065 PubMed39883393

2025

Exploring the Role of TRAF6-TAK1 Pathway in Podocyte Pyroptosis and Its Implications for Primary Membranous Nephropathy Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaling Guo,Jingliang Min,Baochao Chang,Zheng Chen,Weidong Chen

Molecular medicine reports 31: PubMed39635829

2024

SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Yang,Huiping Qin,Chaoqun Qin,Bing Huang,Feng Gao,Yi Liao,Yanping Tang,Yanju Mo,Qianjie Yang,Changming Wang

Renal failure 46:2371992 PubMed39082739

2024

Astragaloside IV relieves passive heymann nephritis and podocyte injury by suppressing the TRAF6/NF-κb axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Ma,Yuwen Hu,Yilin Ruan,Xiaocheng Jiang,Min Zhao,Yuxin Wang,Yanrong Ke,Manman Shi,Guoyuan Lu

Journal of nanobiotechnology 21:419 PubMed37957714

2023

Exosomal miR-146a-5p derived from human umbilical cord mesenchymal stem cells can alleviate antiphospholipid antibody-induced trophoblast injury and placental dysfunction by regulating the TRAF6/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingfeng Lv,Yuan Wang,Wei Tian,Yuqiu Liu,Mengqi Gu,Xiaotong Jiang,Yanjun Cai,Ruiheng Huo,Yuchen Li,Lei Li,Xietong Wang

Chinese medicine 18:143 PubMed37919806

2023

Xinyang tablet ameliorates sepsis-induced myocardial dysfunction by regulating Beclin-1 to mediate macrophage autophagy and M2 polarization through LncSICRNT1 targeting E3 ubiquitin ligase TRAF6.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Luo,Yuanmei Li,Liwei He,Haitao Tu,Xinfeng Lin,Fengli Zhao,Yusheng Huang,Minyong Wen,Lingjun Wang,Zhongqi Yang

Nanomaterials (Basel, Switzerland) 13: PubMed36985977

2023

PF127 Hydrogel-Based Delivery of Exosomal CTNNB1 from Mesenchymal Stem Cells Induces Osteogenic Differentiation during the Repair of Alveolar Bone Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Longlong He,Qin Zhou,Hengwei Zhang,Ningbo Zhao,Lifan Liao

Journal of musculoskeletal & neuronal interactions 22:536-545 PubMed36458391

2022

USP1 Inhibits NF-κB/NLRP3 Induced Pyroptosis through TRAF6 in Osteoblastic MC3T3-E1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dengshuo Sun,Yi Peng,Shengyang Ge,Qiang Fu
View all publications
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