AB245636

Anti-TRAF6BP/TAX1BP1 antibody

Name: Anti-TRAF6BP/TAX1BP1 antibody (ab245636) | Abcam
Brand: Abcam Limited
SKU: AB245636
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal TRAF6BP/TAX1BP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TAX1BP1 aa 700 to C-terminus.

View Alternative Names

T6BP, PRO0105, TAX1BP1, Tax1-binding protein 1, TRAF6-binding protein

2 Images

Supplier Data

Immunoprecipitation - Anti-TRAF6BP/TAX1BP1 antibody (AB245636)

TRAF6BP/TAX1BP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245636 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245636 at 1 μg/ml.

Lane 1 : ab245636 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TRAF6BP/TAX1BP1 antibody (ab245636)

Predicted band size: 91 kDa

false

Supplier Data

Western blot - Anti-TRAF6BP/TAX1BP1 antibody (AB245636)

All lanes:

Western blot - Anti-TRAF6BP/TAX1BP1 antibody (ab245636) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 91 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TAX1BP1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86VP1

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245636 was affinity purified using an epitope specific to TRAF6BP/TAX1BP1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF6BP also known as TAX1BP1 is a cytoplasmic protein that participates in important signaling pathways. It has an approximately 75 kDa molecular weight and mainly expresses in immune-related cells including macrophages and lymphocytes. TRAF6BP works as an adaptor protein binding to TRAF6 and regulating its ubiquitin ligase activity which is key in transmitting signals inside the cell.

Biological function summary

TRAF6BP/TAX1BP1 regulates the immune response through its interaction with TRAF6. It plays a part in the formation of a protein complex that influences the balance between pro-inflammatory and anti-inflammatory signals. TRAF6BP affects autophagy an important process for cellular homeostasis and pathogen elimination. TRAF6BP also manages cell survival and apoptosis through its role in signaling cascades.

Pathways

TRAF6BP/TAX1BP1 is heavily involved in the NF-kB and JNK signaling pathways which are pivotal for immune and inflammatory responses. TRAF6BP interacts with proteins like TRAF6 and TAK1 within these pathways to mediate downstream effects that lead to cytokine production and stress responses. These pathways play large roles in maintaining the immune system's balance.

TRAF6BP/TAX1BP1 has connections with inflammatory conditions and certain cancers. It associates with chronic inflammatory diseases due to its role in regulating cytokine production. Changes in TRAF6BP expression or function can lead to unregulated inflammation which is characteristic of rheumatoid arthritis. Furthermore TRAF6BP's regulatory effect on the NF-kB pathway links it to cancer development particularly in cases where overactive NF-kB signaling promotes tumor growth. Its interaction with TRAF6 is critical in these disease contexts.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ubiquitin-binding adapter that participates in inflammatory, antiviral and innate immune processes as well as selective autophagy regulation (PubMed : 29940186, PubMed : 30459273, PubMed : 30909570). Plays a key role in the negative regulation of NF-kappa-B and IRF3 signalings by acting as an adapter for the ubiquitin-editing enzyme A20/TNFAIP3 to bind and inactivate its substrates (PubMed : 17703191). Disrupts the interactions between the E3 ubiquitin ligase TRAF3 and TBK1/IKBKE to attenuate 'Lys63'-linked polyubiquitination of TBK1 and thereby IFN-beta production (PubMed : 21885437). Recruits also A20/TNFAIP3 to ubiquitinated signaling proteins TRAF6 and RIPK1, leading to their deubiquitination and disruption of IL-1 and TNF-induced NF-kappa-B signaling pathways (PubMed : 17703191). Inhibits virus-induced apoptosis by inducing the 'Lys-48'-linked polyubiquitination and degradation of MAVS via recruitment of the E3 ligase ITCH, thereby attenuating MAVS-mediated apoptosis signaling (PubMed : 27736772). As a macroautophagy/autophagy receptor, facilitates the xenophagic clearance of pathogenic bacteria such as Salmonella typhimurium and Mycobacterium tuberculosis (PubMed : 26451915). Upon NBR1 recruitment to the SQSTM1-ubiquitin condensates, acts as the major recruiter of RB1CC1 to these ubiquitin condensates to promote their autophagic degradation (PubMed : 33226137, PubMed : 34471133). Mediates the autophagic degradation of other substrates including TICAM1 (PubMed : 28898289).

See full target information TAX1BP1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 26:1 PubMed38167446

2024

Protein disulfide isomerase family member 4 promotes triple-negative breast cancer tumorigenesis and radiotherapy resistance through JNK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinqiu Tao,Cailin Xue,Meng Cao,Jiahui Ye,Yulu Sun,Hao Chen,Yinan Guan,Wenjie Zhang,Weijie Zhang,Yongzhong Yao

Nature communications 10:3459 PubMed31371777

2019

Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering.

Applications

Unspecified application

Species

Unspecified reactive species

Shichen Hu,Yujiao Guo,Yingli Wang,Ying Li,Tao Fu,Zixuan Zhou,Yaru Wang,Jianping Liu,Lifeng Pan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAF6BP/TAX1BP1 antibody

Immunoprecipitation - Anti-TRAF6BP/TAX1BP1 antibody (AB245636)

Western blot - Anti-TRAF6BP/TAX1BP1 antibody (AB245636)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise