Rabbit Polyclonal TRAF6BP/TAX1BP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TAX1BP1 aa 700 to C-terminus.
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Publications
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- Breast cancer research : BCR 26:12024Protein disulfide isomerase family member 4 promotes triple-negative breast cancer tumorigenesis and radiotherapy resistance through JNK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinqiu Tao,Cailin Xue,Meng Cao,Jiahui Ye,Yulu Sun,Hao Chen,Yinan Guan,Wenjie Zhang,Weijie Zhang,Yongzhong YaoPubMed 38167446
- Nature communications 10:34592019Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShichen Hu et. al.PubMed 31371777
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TAX1BP1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q86VP1
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
Ubiquitin-binding adapter that participates in inflammatory, antiviral and innate immune processes as well as selective autophagy regulation (PubMed:29940186, PubMed:30459273, PubMed:30909570). Plays a key role in the negative regulation of NF-kappa-B and IRF3 signalings by acting as an adapter for the ubiquitin-editing enzyme A20/TNFAIP3 to bind and inactivate its substrates (PubMed:17703191). Disrupts the interactions between the E3 ubiquitin ligase TRAF3 and TBK1/IKBKE to attenuate 'Lys63'-linked polyubiquitination of TBK1 and thereby IFN-beta production (PubMed:21885437). Recruits also A20/TNFAIP3 to ubiquitinated signaling proteins TRAF6 and RIPK1, leading to their deubiquitination and disruption of IL-1 and TNF-induced NF-kappa-B signaling pathways (PubMed:17703191). Inhibits virus-induced apoptosis by inducing the 'Lys-48'-linked polyubiquitination and degradation of MAVS via recruitment of the E3 ligase ITCH, thereby attenuating MAVS-mediated apoptosis signaling (PubMed:27736772). As a macroautophagy/autophagy receptor, facilitates the xenophagic clearance of pathogenic bacteria such as Salmonella typhimurium and Mycobacterium tuberculosis (PubMed:26451915). Upon NBR1 recruitment to the SQSTM1-ubiquitin condensates, acts as the major recruiter of RB1CC1 to these ubiquitin condensates to promote their autophagic degradation (PubMed:33226137, PubMed:34471133). Mediates the autophagic degradation of other substrates including TICAM1 (PubMed:28898289).
Alternative names
T6BP, PRO0105, TAX1BP1, Tax1-binding protein 1, TRAF6-binding protein
Recommended products
Rabbit Polyclonal TRAF6BP/TAX1BP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TAX1BP1 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TAX1BP1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q86VP1
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab245636 was affinity purified using an epitope specific to TRAF6BP/TAX1BP1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAF6BP also known as TAX1BP1 is a cytoplasmic protein that participates in important signaling pathways. It has an approximately 75 kDa molecular weight and mainly expresses in immune-related cells including macrophages and lymphocytes. TRAF6BP works as an adaptor protein binding to TRAF6 and regulating its ubiquitin ligase activity which is key in transmitting signals inside the cell.
- Biological function summary
TRAF6BP/TAX1BP1 regulates the immune response through its interaction with TRAF6. It plays a part in the formation of a protein complex that influences the balance between pro-inflammatory and anti-inflammatory signals. TRAF6BP affects autophagy an important process for cellular homeostasis and pathogen elimination. TRAF6BP also manages cell survival and apoptosis through its role in signaling cascades.
- Pathways
TRAF6BP/TAX1BP1 is heavily involved in the NF-kB and JNK signaling pathways which are pivotal for immune and inflammatory responses. TRAF6BP interacts with proteins like TRAF6 and TAK1 within these pathways to mediate downstream effects that lead to cytokine production and stress responses. These pathways play large roles in maintaining the immune system's balance.
- Associated diseases and disorders
TRAF6BP/TAX1BP1 has connections with inflammatory conditions and certain cancers. It associates with chronic inflammatory diseases due to its role in regulating cytokine production. Changes in TRAF6BP expression or function can lead to unregulated inflammation which is characteristic of rheumatoid arthritis. Furthermore TRAF6BP's regulatory effect on the NF-kB pathway links it to cancer development particularly in cases where overactive NF-kB signaling promotes tumor growth. Its interaction with TRAF6 is critical in these disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-TRAF6BP/TAX1BP1 antibody (ab245636)
All lanes: Western blot - Anti-TRAF6BP/TAX1BP1 antibody (ab245636) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 30s
Immunoprecipitation - Anti-TRAF6BP/TAX1BP1 antibody (ab245636)
TRAF6BP/TAX1BP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245636 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245636 at 1 μg/ml.
Lane 1: ab245636 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-TRAF6BP/TAX1BP1 antibody (ab245636)
Predicted band size: 91 kDa
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