AB231063

Anti-TRAIL antibody

Name: Anti-TRAIL antibody (ab231063) | Abcam
Brand: Abcam Limited
SKU: AB231063
Price: 525 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal TRAIL antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 7 publications.

View Alternative Names

CD253, APO2L, TRAIL, TNFSF10, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis-inducing ligand, Apo-2L, Protein TRAIL

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Formalin-fixed, paraffin-embedded rat ovary tissue stained for TRAIL with ab231063 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Formalin-fixed, paraffin-embedded rat stomach tissue stained for TRAIL with ab231063 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Formalin-fixed, paraffin-embedded rat intestine tissue stained for TRAIL with ab231063 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Formalin-fixed, paraffin-embedded rat kidney tissue stained for TRAIL with ab231063 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Formalin-fixed, paraffin-embedded rat testis tissue stained for TRAIL with ab231063 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-TRAIL antibody (AB231063)

All lanes:

Western blot - Anti-TRAIL antibody (ab231063) at 3 µg/mL

All lanes:

MCF7 (Human breast adenocarcinoma cell line) cell lysate

Secondary

All lanes:

HRP-linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 32 kDa

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Supplier Data

Western blot - Anti-TRAIL antibody (AB231063)

All lanes:

Western blot - Anti-TRAIL antibody (ab231063) at 2 µg/mL

All lanes:

recombinant rat TRAIL protein

Predicted band size: 32 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.

Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed : 10549288, PubMed : 26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

See full target information TNFSF10

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 30:1-8 PubMed40476159

2025

Transplantation of chemically induced liver progenitors in Nagase analbuminemic rats under liver regenerative stimulus.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Fukumoto,Akihiko Soyama,Daisuke Miyamoto,Takanobu Hara,Hajime Matsushima,Hajime Imamura,Mampei Yamashita,Tomohiko Adachi,Kengo Kanetaka,Susumu Eguchi

Cell death & disease 15:595 PubMed39152108

2024

Autophagy modulation attenuates sorafenib resistance in HCC induced in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yomna Elleithi,Amal El-Gayar,Mohamed N Amin

Orphanet journal of rare diseases 18:66 PubMed36959587

2023

Comprehensive analysis of autophagy-related gene expression profiles identified five gene biomarkers associated with immune infiltration and advanced plaques in carotid atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Ma,Taoyuan Lu,Yanyan He,Dehua Guo,Lin Duan,Rufeng Jia,Dongyang Cai,Tao Gao,Zhongcan Chen,Binghua Xue,Tianxiao Li,Yingkun He

Journal of immunology research 2023:5293677 PubMed36969496

2023

Network-Based Method to Investigate the Promoted Cell Apoptosis Mechanisms of Oridonin in OSCC through the RNA-Transcriptome.

Applications

Unspecified application

Species

Unspecified reactive species

Guohui Wu,Yusheng Guo,Yang Liu,Xiangsheng Cai,Tanggang Deng,Tianchu Pei,Long Huang,Kai Chen,Xuan Pan

Oxidative medicine and cellular longevity 2020:6196398 PubMed32566090

2020

Moderate Mechanical Stimulation Protects Rats against Osteoarthritis through the Regulation of TRAIL via the NF-B/NLRP3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Yang,Yang Wang,Yawei Kong,Xiaoning Zhang,He Zhang,Xinyuan Feng,Ziyuan Wang,Peng Gao,Mingyue Yan,Lunhao Bai,Feng Li

Nature communications 10:5183 PubMed31729368

2019

A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine D Arnold,Josephine A Pickworth,Laura E West,Sarah Dawson,Joana A Carvalho,Helen Casbolt,Adam T Braithwaite,James Iremonger,Lewis Renshall,Volker Germaschewski,Matthew McCourt,Philip Bland-Ward,Hager Kowash,Abdul G Hameed,Alexander M K Rothman,Maria G Frid,A A Roger Thompson,Holly R Evans,Mark Southwood,Nicholas W Morrell,David C Crossman,Moira K B Whyte,Kurt R Stenmark,Christopher M Newman,David G Kiely,Sheila E Francis,Allan Lawrie

Experimental and therapeutic medicine 17:41-50 PubMed30651763

2019

Tumor necrosis factor-related apoptosis-inducing ligand inhibits the growth and aggressiveness of colon carcinoma via the exogenous apoptosis signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Gong,Weicai Cheng,Yong Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAIL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231063)

Western blot - Anti-TRAIL antibody (AB231063)

Western blot - Anti-TRAIL antibody (AB231063)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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