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AB231265

Anti-TRAIL antibody

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(8 Publications)

Rabbit Polyclonal TRAIL antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Mouse, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tnfsf10 aa 1 to C-terminus.

View Alternative Names

CD253, APO2L, TRAIL, TNFSF10, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis-inducing ligand, Apo-2L, Protein TRAIL

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231265)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (AB231265)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for TRAIL with ab231265 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-TRAIL antibody (AB231265)
  • WB

Supplier Data

Western blot - Anti-TRAIL antibody (AB231265)

All lanes:

Western blot - Anti-TRAIL antibody (ab231265) at 2 µg/mL

All lanes:

A549 (Human lung carcinoma cell line) cell lysate

Predicted band size: 32 kDa

false

Western blot - Anti-TRAIL antibody (AB231265)
  • WB

Supplier Data

Western blot - Anti-TRAIL antibody (AB231265)

All lanes:

Western blot - Anti-TRAIL antibody (ab231265) at 2 µg/mL

All lanes:

Recombinant mouse TRAIL protein

Predicted band size: 32 kDa,55 kDa

false

Western blot - Anti-TRAIL antibody (AB231265)
  • WB

Supplier Data

Western blot - Anti-TRAIL antibody (AB231265)

All lanes:

Western blot - Anti-TRAIL antibody (ab231265) at 2 µg/mL

All lanes:

MCF7 (Human breast adenocarcinoma cell line) cell lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Tnfsf10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P50592

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant full length protein - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.
Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed : 10549288, PubMed : 26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
See full target information TNFSF10
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 20:15 PubMed39918606

2025

Rebalancing Immune Interactions within the Brain-Spleen Axis Mitigates Neuroinflammation in an Aging Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Flavia Cantone,Chiara Burgaletto,Giulia Di Benedetto,Gabriella Gaudio,Cesarina Giallongo,Rosario Caltabiano,Giuseppe Broggi,Carlo Maria Bellanca,Giuseppina Cantarella,Renato Bernardini

Cellular and molecular gastroenterology and hepatology 17:853-876 PubMed38219900

2024

Noncanonical TRAIL Signaling Promotes Myeloid-Derived Suppressor Cell Abundance and Tumor Growth in Cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Emilien J Loeuillard,Binbin Li,Hannah E Stumpf,Jingchun Yang,Jessica R Willhite,Jennifer L Tomlinson,Fred Rakhshan Rohakhtar,Vernadette A Simon,Rondell P Graham,Rory L Smoot,Haidong Dong,Sumera I Ilyas

Cancer medicine 12:20380-20395 PubMed37843231

2023

The tumor suppressive effect and apoptotic mechanism of TRAIL gene-containing recombinant NDV in TRAIL-resistant colorectal cancer HT-29 cells and TRAIL-nonresistant HCT116 cells, with each cell bearing a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Kyoung Jung,Yong Hee An,Sung Hoon Jang,Gyoungah Ryu,Saet-Byel Jung,Seonhee Kim,Cuk-Seong Kim,Hyun Jang

Frontiers in pharmacology 14:1171293 PubMed37274104

2023

Blocking TRAIL-DR5 signaling pathway with soluble death receptor 5 fusion protein mitigates radiation-induced injury.

Applications

Unspecified application

Species

Unspecified reactive species

Danyang Zhao,Lei Yang,Peng Han,Haihui Zhang,Fanjun Wang,Zhiyun Meng,Hui Gan,Zhuona Wu,Wenzhong Sun,Chuan Chen,Guifang Dou,Ruolan Gu

Biomolecules 13: PubMed37238701

2023

Lipoxin A4 (LXA4) Reduces Alkali-Induced Corneal Inflammation and Neovascularization and Upregulates a Repair Transcriptome.

Applications

Unspecified application

Species

Unspecified reactive species

Jiucheng He,Thang L Pham,Azucena H Kakazu,Abhilash Ponnath,Khanh V Do,Haydee E P Bazan

The Journal of clinical investigation 132: PubMed36136587

2022

SIRT1 inactivation switches reactive astrocytes to an antiinflammatory phenotype in CNS autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Zhang,Dan Xiao,Xing Li,Yuan Zhang,Javad Rasouli,Giacomo Casella,Alexandra Boehm,Daniel Hwang,Larissa Lw Ishikawa,Rodolfo Thome,Bogoljub Ciric,Mark T Curtis,Abdolmohamad Rostami,Guang-Xian Zhang

American journal of cancer research 12:2118-2131 PubMed35693092

2022

Oral administration of TRAIL-inducing small molecule ONC201/TIC10 prevents intestinal polyposis in the mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Venkateshwar Madka,Arielle De La Cruz,Gopal Pathuri,Janani Panneerselvam,Yuting Zhang,Nicole Stratton,Sean Hacking,Niklas K Finnberg,Howard P Safran,Shizuko Sei,Elizabeth R Glaze,Robert Shoemaker,Jennifer T Fox,Alexander G Raufi,Wafik S El-Deiry,Chinthalapally V Rao

Journal of cellular physiology 235:2676-2686 PubMed31508816

2019

MicroRNA-181a exerts anti-inflammatory effects via inhibition of the ERK pathway in mice with intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yanpeng Sun,Xiangqin Shi,Xiaodong Peng,Yanzhou Li,Husheng Ma,Dongfang Li,Xiangyang Cao
View all publications
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