AB9959

Anti-TRAIL antibody

Name: Anti-TRAIL antibody (ab9959) | Abcam
Brand: Abcam Limited
SKU: AB9959
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(14 Publications)

Rabbit Polyclonal TRAIL antibody. Suitable for sELISA and reacts with Recombinant fragment - Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNFSF10 aa 100 to C-terminus.

View Alternative Names

CD253, APO2L, TRAIL, TNFSF10, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis-inducing ligand, Apo-2L, Protein TRAIL

1 Images

Sandwich ELISA - Anti-TRAIL antibody (AB9959)

sELISA

Unknown

Sandwich ELISA - Anti-TRAIL antibody (AB9959)

Standard curve for TRAIL (Analyte : ab78818); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [55B709.3] to TRAIL (ab12124) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to TRAIL (ab9959) at 0.1μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

sELISA

applications

Immunogen

Recombinant Fragment Protein within Human TNFSF10 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P50591

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant fragment - Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>To detect TRAIL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with <a href='/en-us/products/unavailable/trail-antibody-55b7093-ab12124'>ab12124</a> as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant TRAIL.</p>" } } }

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Product details

Although some customers have been successful in IHC we no longer batch test in this application.

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute with 200µl of sterile water.

Purification technique

Affinity purification Immunogen

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.

Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed : 10549288, PubMed : 26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

See full target information TNFSF10

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Bioorganic chemistry 127:105926 PubMed35717804

2022

Structure-activity relationships reveal a 2-furoyloxychalcone as a potent cytotoxic and apoptosis inducer for human U-937 and HL-60 leukaemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Henoc Del Rosario,Ester Saavedra,Ignacio Brouard,Daniel González-Santana,Celina García,Elena Spínola-Lasso,Carlos Tabraue,José Quintana,Francisco Estévez

International journal of molecular sciences 22: PubMed34948260

2021

Apoptosis Pathways Triggered by a Potent Antiproliferative Hybrid Chalcone on Human Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Rodríguez,Ester Saavedra,Henoc Del Rosario,Juan Perdomo,José Quintana,Filippo Prencipe,Paola Oliva,Romeo Romagnoli,Francisco Estévez

Journal of clinical medicine 10: PubMed34203833

2021

Engineering of CD19 Antibodies: A CD19-TRAIL Fusion Construct Specifically Induces Apoptosis in B-Cell Precursor Acute Lymphoblastic Leukemia (BCP-ALL) Cells In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Dorothee Winterberg,Lennart Lenk,Maren Oßwald,Fotini Vogiatzi,Carina Lynn Gehlert,Fabian-Simon Frielitz,Katja Klausz,Thies Rösner,Thomas Valerius,Anna Trauzold,Matthias Peipp,Christian Kellner,Denis Martin Schewe

Cancer chemotherapy and pharmacology 88:289-306 PubMed33942150

2021

NCTR fusion facilitates the formation of TRAIL polymers that selectively activate TRAIL receptors with higher potency and efficacy than TRAIL.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Qiong Lei,Cangjie Shen,Nan Wang

Science advances 7: PubMed33658202

2021

Anti-EGFR VHH-armed death receptor ligand-engineered allogeneic stem cells have therapeutic efficacy in diverse brain metastatic breast cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Yohei Kitamura,Nobuhiko Kanaya,Susana Moleirinho,Wanlu Du,Clemens Reinshagen,Nada Attia,Agnieszka Bronisz,Esther Revai Lechtich,Hikaru Sasaki,Joana Liliana Mora,Priscilla Kaliopi Brastianos,Jefferey L Falcone,Aldebaran M Hofer,Arnaldo Franco,Khalid Shah

Signal transduction and targeted therapy 6:4 PubMed33414378

2021

Reprogramming immunosuppressive myeloid cells by activated T cells promotes the response to anti-PD-1 therapy in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Hong-Wei Sun,Yan-Yan Yang,Hai-Tian Chen,Xing-Juan Yu,Wen-Chao Wu,Yi-Tuo Xu,Li-Lian Jin,Xiao-Jun Wu,Jing Xu,Limin Zheng

Scientific reports 10:19997 PubMed33203916

2020

Designed ferritin nanocages displaying trimeric TRAIL and tumor-targeting peptides confer superior anti-tumor efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Do Yoo,Sang Mun Bae,Junyoung Seo,In Seon Jeon,Sri Murugan Poongkavithai Vadevoo,Sang-Yeob Kim,In-San Kim,Byungheon Lee,Soyoun Kim

Immunity, inflammation and disease 9:144-156 PubMed33156578

2020

Secreted TRAIL gene-modified adipose-derived stem cells exhibited potent tumor-suppressive effect in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Liu,Shaojie Li,Tiexiang Ma,Jian Zeng,Xin Zhou,Huanyu Li,Min Tang,Xiang Liu,Feng Li,Bin Jiang,Ming Zhao,Ying Chen

Biochemistry 59:3856-3868 PubMed32941010

2020

Noncompetitive Allosteric Antagonism of Death Receptor 5 by a Synthetic Affibody Ligand.

Applications

Unspecified application

Species

Unspecified reactive species

Nagamani Vunnam,Sophia Szymonski,Petra Hirsova,Gregory J Gores,Jonathan N Sachs,Benjamin J Hackel

Biomaterials science 8:5317-5328 PubMed32779647

2020

Biosilicified oncolytic adenovirus for cancer viral gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Kong,Ruibo Zhao,Quan Zhang,Muhammed Zubair Iqbal,Jiaju Lu,Qingwei Zhao,Dandan Luo,Cui Feng,Kangjian Zhang,Xinyuan Liu,Xiangdong Kong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAIL antibody

Sandwich ELISA - Anti-TRAIL antibody (AB9959)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Reconstitution

Purification technique

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise