JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB323703

Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TRAIL antibody. Carrier free. Suitable for Flow Cyt (Intra), Flow Cyt, WB and reacts with Transfected cell line - Mouse, Mouse samples.

View Alternative Names

CD253, Trail, Tnfsf10, Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

6 Images
Flow Cytometry (Intracellular) - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T (human embryonic kidney epithelial cell) cells transfected with a mouse TRAIL expression vector containing a Myc tag (Middle) /293T cells transfected with an empty expression vector containing a Myc tag (Right) cells labelling TRAIL with ab323702 at 1/5000 dilution (0.01 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control (ab172730) or ab323702. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor®647.

Flow Cytometry - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell cells labelling TRAIL with ab323702 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse spleen cells were co-stained with anti mouse CD19 conjugated to PE/Cy7.
Gated on viable cell.

Flow Cytometry - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell cells labelling TRAIL with ab323702 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse spleen cells were co-stained with anti mouse CD11b conjugated to BV421.
Gated on viable cell.

Flow Cytometry - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell cells labelling TRAIL with ab323702 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse spleen cells were co-stained with anti mouse CD3 conjugated to Alexa Fluor®647.
Gated on viable cell.

Western blot - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)
  • WB

Supplier Data

Western blot - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of TRAIL is induced by LPS treatment (PMID : 9603453).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TRAIL antibody [EPR28587-33] (<a href='/en-us/products/primary-antibodies/trail-antibody-epr28587-33-ab323702'>ab323702</a>) at 1/1000 dilution

Lane 1:

Untreated mouse lung tissue lysate at 40 µg

Lane 2:

Mouse lung treated with 1ug/ml LPS and 1ug/ml BFA for 16h whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28-30 kDa,36 kDa

false

Exposure time: 48s

Western blot - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)
  • WB

Supplier Data

Western blot - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of TRAIL is induced by LPS treatment (PMID : 9603453).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TRAIL antibody [EPR28587-33] (<a href='/en-us/products/primary-antibodies/trail-antibody-epr28587-33-ab323702'>ab323702</a>) at 1/1000 dilution

Lane 1:

Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 40 µg

Lane 2:

RAW 264.7 treated with 100 ng/ml LPS for 6h and 300ng/ml BFA for 3h whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 28-30 kDa,36 kDa

false

Exposure time: 26s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28587-33

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt (Intra), WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB46074

Human TRAIL ELISA Kit (CD253)

ELISA - Human TRAIL ELISA Kit (CD253) (AB46074)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Transfected cell line - Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab323703 is the carrier-free version of ab323702

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
See full target information Tnfsf10
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com