Anti-TRAK1 antibody [EPR30574-567] - BSA and Azide free

• WB

Lab

Western blot - Anti-TRAK1 antibody [EPR30574-567] - BSA and Azide free (AB326969)

This data was developed using ab327353, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human TRAK2.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa);Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(130KDa).

All lanes:

Western blot - Anti-TRAK1 antibody [EPR30574-567] (<a href='/en-us/products/primary-antibodies/trak1-antibody-epr30574-567-ab327353'>ab327353</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human TRAK1 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human TRAK2 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 8s

• WB

Lab

Western blot - Anti-TRAK1 antibody [EPR30574-567] - BSA and Azide free (AB326969)

This data was developed using ab327353, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands between 37 kDa and 75 kDa (in lanes 1-2) are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-TRAK1 antibody [EPR30574-567] (<a href='/en-us/products/primary-antibodies/trak1-antibody-epr30574-567-ab327353'>ab327353</a>) at 1/1000 dilution

Lane 1:

Untreated SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 80 µg

Lane 2:

SH-SY5Y neuron differentiation for 14 days whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-TRAK1 antibody [EPR30574-567] - BSA and Azide free (AB326969)

This data was developed using ab327353, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen.

The lanes 1-2 were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

The identity of the bands between 37 kDa and 75 kDa (in lanes 1-2) are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes 1-2 : 92 seconds, lanes 3-6 : 180 seconds

All lanes:

Western blot - Anti-TRAK1 antibody [EPR30574-567] (<a href='/en-us/products/primary-antibodies/trak1-antibody-epr30574-567-ab327353'>ab327353</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 80 µg

Lane 2:

Human spleen tissue lysate at 80 µg

Lane 3:

Mouse spinal cord tissue lysate at 80 µg

Lane 4:

Mouse hippocampus tissue lysate at 80 µg

Lane 5:

Mouse cerebellum tissue lysate at 80 µg

Lane 6:

Mouse spleen tissue lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 130 kDa,36 kDa

true

• WB

Lab

Western blot - Anti-TRAK1 antibody [EPR30574-567] - BSA and Azide free (AB326969)

This data was developed using ab327353, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Molt-4.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-TRAK1 antibody [EPR30574-567] (<a href='/en-us/products/primary-antibodies/trak1-antibody-epr30574-567-ab327353'>ab327353</a>) at 1/1000 dilution

Lane 1:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 80 µg

Lane 2:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 80 µg

Lane 3:

Mouse primary neuron whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-TRAK1 antibody [EPR30574-567] - BSA and Azide free (AB326969)

This data was developed using ab327353, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen.

To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-TRAK1 antibody [EPR30574-567] (<a href='/en-us/products/primary-antibodies/trak1-antibody-epr30574-567-ab327353'>ab327353</a>) at 1/1000 dilution

Lane 1:

Mouse brain fresh tissue lysate at 80 µg

Lane 2:

Mouse spleen fresh tissue lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 130 kDa,124 kDa

false

Exposure time: 180s