AB96106

Anti-TRAM1/TRAM antibody

Name: Anti-TRAM1/TRAM antibody (ab96106) | Abcam
Brand: Abcam Limited
SKU: AB96106

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(3 Publications)

Rabbit Polyclonal TRAM1/TRAM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRAM1 aa 300 to C-terminus.

View Alternative Names

TRAM, TRAM1, Translocating chain-associated membrane protein 1, Protein TRAM1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAM1/TRAM antibody (AB96106)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAM1/TRAM antibody (AB96106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of AGS xenograft staining TRAM1 with ab96106 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TRAM1/TRAM antibody (AB96106)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRAM1/TRAM antibody (AB96106)

Immunocytochemistry/ Immunofluorescence of methanol-fixed HeLa cells staining TRAM1 with ab96106 at 1/500 dilution (green). Costained with Hoechst 33342 (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAM1/TRAM antibody (AB96106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse intestine staining TRAM1 with ab96106 at 1/500 dilution.

Unknown

Western blot - Anti-TRAM1/TRAM antibody (AB96106)

10% SDS-PAGE

All lanes:

Western blot - Anti-TRAM1/TRAM antibody (ab96106) at 1/1000 dilution

All lanes:

H1299 cell lysate at 30 µg

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TRAM1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15629

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAM1 also known as TRAM is a translocation-associated membrane protein involved in the translation and insertion of nascent polypeptides into the endoplasmic reticulum (ER). This protein plays an important role in directing proteins into the ER membrane or lumen via the translocon complex. TRAM1 has a molecular mass of approximately 37 kDa and is widely expressed especially in tissues with high secretory activity such as the liver pancreas and intestine.

Biological function summary

TRAM1 interacts with ribosomes and assists in the co-translational translocation of polypeptides across the endoplasmic reticulum membrane. It forms part of the larger translocon complex which includes Sec61 and other associated proteins. These interactions are necessary for accurate protein targeting and membrane integration ensuring newly synthesized proteins reach their intended cellular compartments.

Pathways

TRAM1 is important for protein secretion and membrane protein biosynthesis pathways. It collaborates closely with Sec61 facilitating protein entry into the ER. It also interfaces with the signal recognition particle (SRP) pathway guiding ribosome-bound nascent chains to the ER membrane. These pathways are essential for proper protein folding processing and eventual function impacting cell signaling and homeostasis.

Mutations or dysfunctions in TRAM1 have potential links to disorders such as congenital disorders of glycosylation and certain liver diseases. Altered interactions with proteins like Sec61 can disrupt normal protein processing pathways contributing to cellular stress and pathology. TRAM1's role in the protein translocation process makes it an important subject of study for understanding the molecular basis of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in the translocation of nascent protein chains into or through the endoplasmic reticulum (ER) membrane by facilitating the proper chain positioning at the SEC61 channel (PubMed : 12475939, PubMed : 1315422, PubMed : 32013668, PubMed : 8616892, PubMed : 9506517). Regulates the exposure of nascent secretory protein chain to the cytosol during translocation into the ER (PubMed : 9506517). May affect the phospholipid bilayer in the vicinity of the lateral gate of the SEC61 channel, thereby facilitating ER protein transport (PubMed : 32013668). Intimately associates with transmembrane (TM) domain of nascent membrane proteins during the entire integration process into the ER membrane (PubMed : 8616892). Associates with the second TM domain of G-protein-coupled receptor opsin/OPSD nascent chain in the ER membrane, which may facilitate its integration into the membrane (PubMed : 12475939). Under conditions of ER stress, participates in the disposal of misfolded ER membrane proteins during the unfolded protein response (UPR), an integrated stress response (ISR) pathway, by selectively retrotranslocating misfolded ER-membrane proteins from the ER into the cytosol where they are ubiquitinated and degraded by the proteasome (PubMed : 20430023).. (Microbial infection) In case of cytomegalovirus infection, participates in US2- and US11-mediated ER-to-cytosol retrotranslocation and subsequent degradation of major histocompatibility complex (MHC) class I heavy chains, thereby decreasing the immune detection by cytotoxic T-cells.

See full target information TRAM1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:4115 PubMed31511519

2019

Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wu,Caoqi Lei,Tian Xia,Xuan Zhong,Qing Yang,Hong-Bing Shu

PLoS pathogens 13:e1006397 PubMed28542576

2017

Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection.

Applications

Unspecified application

Species

Unspecified reactive species

Mathieu Iampietro,Patrick Younan,Andrew Nishida,Mukta Dutta,Ndongala Michel Lubaki,Rodrigo I Santos,Richard A Koup,Michael G Katze,Alexander Bukreyev

PloS one 7:e44500 PubMed22970235

2012

Human cytomegalovirus induces TLR4 signaling components in monocytes altering TIRAP, TRAM and downstream interferon-beta and TNF-alpha expression.

Applications

Species

Unspecified reactive species

Kok-Hooi Yew,Cory Carpenter,R Scott Duncan,Christopher J Harrison

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TRAM1/TRAM antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAM1/TRAM antibody (AB96106)

Immunocytochemistry/ Immunofluorescence - Anti-TRAM1/TRAM antibody (AB96106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAM1/TRAM antibody (AB96106)

Western blot - Anti-TRAM1/TRAM antibody (AB96106)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise