Rabbit Polyclonal TRAM1/TRAM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRAM1 aa 300 to C-terminus.
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Publications
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- Nature communications 10:41152019Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Wu et. al.PubMed 31511519
- PLoS pathogens 13:e10063972017Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMathieu Iampietro et. al.PubMed 28542576
- PloS one 7:e445002012Human cytomegalovirus induces TLR4 signaling components in monocytes altering TIRAP, TRAM and downstream interferon-beta and TNF-alpha expression.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TRAM1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15629
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Involved in the translocation of nascent protein chains into or through the endoplasmic reticulum (ER) membrane by facilitating the proper chain positioning at the SEC61 channel (PubMed:12475939, PubMed:1315422, PubMed:32013668, PubMed:8616892, PubMed:9506517). Regulates the exposure of nascent secretory protein chain to the cytosol during translocation into the ER (PubMed:9506517). May affect the phospholipid bilayer in the vicinity of the lateral gate of the SEC61 channel, thereby facilitating ER protein transport (PubMed:32013668). Intimately associates with transmembrane (TM) domain of nascent membrane proteins during the entire integration process into the ER membrane (PubMed:8616892). Associates with the second TM domain of G-protein-coupled receptor opsin/OPSD nascent chain in the ER membrane, which may facilitate its integration into the membrane (PubMed:12475939). Under conditions of ER stress, participates in the disposal of misfolded ER membrane proteins during the unfolded protein response (UPR), an integrated stress response (ISR) pathway, by selectively retrotranslocating misfolded ER-membrane proteins from the ER into the cytosol where they are ubiquitinated and degraded by the proteasome (PubMed:20430023). (Microbial infection) In case of cytomegalovirus infection, participates in US2- and US11-mediated ER-to-cytosol retrotranslocation and subsequent degradation of major histocompatibility complex (MHC) class I heavy chains, thereby decreasing the immune detection by cytotoxic T-cells.
Alternative names
TRAM, TRAM1, Translocating chain-associated membrane protein 1, Protein TRAM1
Recommended products
Rabbit Polyclonal TRAM1/TRAM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRAM1 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TRAM1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15629
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAM1 also known as TRAM is a translocation-associated membrane protein involved in the translation and insertion of nascent polypeptides into the endoplasmic reticulum (ER). This protein plays an important role in directing proteins into the ER membrane or lumen via the translocon complex. TRAM1 has a molecular mass of approximately 37 kDa and is widely expressed especially in tissues with high secretory activity such as the liver pancreas and intestine.
- Biological function summary
TRAM1 interacts with ribosomes and assists in the co-translational translocation of polypeptides across the endoplasmic reticulum membrane. It forms part of the larger translocon complex which includes Sec61 and other associated proteins. These interactions are necessary for accurate protein targeting and membrane integration ensuring newly synthesized proteins reach their intended cellular compartments.
- Pathways
TRAM1 is important for protein secretion and membrane protein biosynthesis pathways. It collaborates closely with Sec61 facilitating protein entry into the ER. It also interfaces with the signal recognition particle (SRP) pathway guiding ribosome-bound nascent chains to the ER membrane. These pathways are essential for proper protein folding processing and eventual function impacting cell signaling and homeostasis.
- Associated diseases and disorders
Mutations or dysfunctions in TRAM1 have potential links to disorders such as congenital disorders of glycosylation and certain liver diseases. Altered interactions with proteins like Sec61 can disrupt normal protein processing pathways contributing to cellular stress and pathology. TRAM1's role in the protein translocation process makes it an important subject of study for understanding the molecular basis of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-TRAM1/TRAM antibody (ab96106)
10% SDS-PAGE
All lanes: Western blot - Anti-TRAM1/TRAM antibody (ab96106) at 1/1000 dilution
All lanes: H1299 cell lysate at 30 µg
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAM1/TRAM antibody (ab96106)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse intestine staining TRAM1 with ab96106 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TRAM1/TRAM antibody (ab96106)
Immunocytochemistry/ Immunofluorescence of methanol-fixed HeLa cells staining TRAM1 with ab96106 at 1/500 dilution (green). Costained with Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAM1/TRAM antibody (ab96106)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of AGS xenograft staining TRAM1 with ab96106 at 1/500 dilution.
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