Rabbit Recombinant Monoclonal Trans-Golgi network integral membrane protein 1 antibody. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse samples.
Images
![Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--western-blot-img165698.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678)")
![Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--western-blot-img170286.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165366.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img173167.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164476.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (AB283678)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Target data
Function
May be involved in regulating membrane traffic to and from trans-Golgi network.
Alternative names
Ttgn1, Tgoln1, Trans-Golgi network integral membrane protein 1, TGN38A
Recommended products
Rabbit Recombinant Monoclonal Trans-Golgi network integral membrane protein 1 antibody. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24831-36
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Trans-Golgi network integral membrane protein 1 also known by the acronym TGOLN2 or TGN46 is an integral membrane protein that plays a critical role in the sorting and trafficking of proteins. The protein has a molecular mass of about 46 kDa and resides primarily in the trans-Golgi network of eukaryotic cells. It is expressed in various tissues with prominent expression in cells featuring extensive secretory pathways such as liver and pancreatic cells. This positioning enables it to fulfill its role in mediating the transport of proteins from the Golgi apparatus to their specific cellular destinations.
- Biological function summary
The protein is involved in maintaining the structure and function of the trans-Golgi network. TGOLN2 works as part of a complex network that includes multiple integral membrane proteins which regulate vesicular trafficking. Its ability to bind with other proteins facilitates the sorting and packaging of secretory proteins which are then dispatched through vesicles. By efficiently working with the cellular machinery TGOLN2 contributes to the proper processing and delivery of proteins essential for cellular function.
- Pathways
TGOLN2 significantly integrates in the protein trafficking and glycosylation pathways within cells. It cooperates closely with Kinesin and Dynein motor proteins to move vesicles along cytoskeletal tracks to their correct location. TGOLN2 also interacts with adaptin protein complexes which are important for the selection of cargo proteins destined for delivery to specific compartments. This positioning in pathways makes TGOLN2 an essential factor in ensuring the precise cellular localization of proteins.
- Associated diseases and disorders
Dysregulation of TGOLN2 expression has been linked to certain neurodegenerative disorders and cancers. Altered functioning in TGOLN2 may contribute to diseases like Alzheimer's by influencing the abnormal accumulation of amyloid precursor protein which TGOLN2 is known to interact with. Additionally cancer cells often show altered protein trafficking mechanisms where the disruption of TGOLN2 can impact tumor progression and metastasis. Understanding TGOLN2's interactions may provide insights into the pathogenesis of these conditions offering potential therapeutic avenues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--western-blot-img165698.jpg "Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lane 1 of this blot was developed using a higher sensitivity ECL substrate.
The expression profiles/observed MW are consistent with what has been described in the literature (PMID: 12494998).
Exposure time: 3 minutes
All lanes: Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 2: C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 85 kDa
![Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--western-blot-img170286.jpg "Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody has cross-reaction with mouse TGN38B.
The recombinant proteins TGN38B and TGN38 were made in-house.
The sample loaded onto lane 1 was purified TGN38B recombinant protein expressed from an E.coli expression system.
The sample loaded onto lanes 2 was purified TGN38 recombinant protein expressed from a mammalian - HEK-293 expression system.
Exposure time: 81 seconds
All lanes: Western blot - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678) at 1/1000 dilution
Lane 1: His-tagged mouse TGN38B recombinant protein at 0.01 µg
Lane 2: LIF-tagged mouse TGN38 recombinant protein at 0.01 µg
SecondaryLane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lane 2: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 50 kDa, 65-100 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165366.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling TGN38 with ab283678 at 1/2000 (0.285 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining in mouse cerebrum. The section was incubated with ab283678 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img173167.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling TGN38 with ab283678 at 1/2000 (0.285 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining in mouse kidney. The section was incubated with ab283678 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164476.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling TGN38 with ab283678 at 1/2000 (0.285 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Granular cytoplasmic staining in mouse liver. The section was incubated with ab283678 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunocytochemistry-immunofluorescence-img168445.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling TGN38 with ab283678 at 1/50 (11.38 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing co-locolization positive staining with Golgi marker in NIH/3T3 cells is observed. Anti-GM130 antibody - BSA and Azide free ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain tubulin at 1/200 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunocytochemistry-immunofluorescence-img171307.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized C2C12 cells labelling TGN38 with ab283678 at 1/50 (11.38 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing co-locolization positive staining with Golgi marker in C2C12 cells is observed. Anti-GM130 antibody - BSA and Azide free ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain tubulin at 1/200 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunoprecipitation - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunoprecipitation-img168484.jpg "Immunoprecipitation - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Immunoprecipitation - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
TGN38 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast), whole cell lysate 10 ug with ab283678 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283678 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: C2C12 (mouse myoblasts myoblast), whole cell lysate 10 ug
Lane 2: ab283678 IP in C2C12 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab283678 in C2C12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 49 seconds
This blot was developed using a higher sensitivity ECL substrate.
All lanes: Immunoprecipitation - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Predicted band size: 38 kDa
Observed band size: 75 kDa
![Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--flow-cytometry-intracellular-img180319.jpg "Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling TGN38 with ab283678 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, Goat F(ab')2 Anti-Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab98507) at 1/500 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--flow-cytometry-intracellular-img168431.jpg "Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labelling TGN38 with ab283678 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, Goat F(ab')2 Anti-Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab98507) at 1/500 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunocytochemistry-immunofluorescence-img168445.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
![Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunocytochemistry-immunofluorescence-img171307.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
![Immunoprecipitation - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--immunoprecipitation-img168484.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
![Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--flow-cytometry-intracellular-img180319.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
![Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678), expandable thumbnail](https://content.abcam.com/products/images/trans-golgi-network-integral-membrane-protein-1-antibody-epr24831-36-ab283678--flow-cytometry-intracellular-img168431.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Trans-Golgi network integral membrane protein 1 antibody [EPR24831-36] (ab283678)")
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