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AB315024

Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free

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Rabbit Recombinant Monoclonal Transaldolase 1 antibody. Carrier free. Suitable for WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

View Alternative Names

TAL, TALDO, TALDOR, TALDO1, Transaldolase

15 Images
Immunocytochemistry/ Immunofluorescence - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with ab315023 at 1/50 (10.14 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing nuclear staining in A431 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Flow Cytometry (Intracellular) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with ab315023 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Transaldolase 1 with ab315023 at 1/8000 (0.063 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab315023 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Transaldolase 1 with ab315023 at 1/8000 (0.063 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human hepatocellular carcinoma. The section was incubated with ab315023 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Immunoprecipitation - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • IP

Supplier Data

Immunoprecipitation - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Transaldolase 1 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate with ab315023 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315023 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

Lane 2 : ab315023 IP in A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315023 in A431 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Transaldolase 1 antibody [EPR28140-51] (<a href='/en-us/products/primary-antibodies/transaldolase-1-antibody-epr28140-51-ab315023'>ab315023</a>) at 1/30 dilution

All lanes:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

Immunocytochemistry/ Immunofluorescence - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) cells labelling Transaldolase 1 with ab315023 at 1/50 (10.14 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing nuclear staining in C6 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Transaldolase 1 with ab315023 at 1/8000 (0.063 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat liver. The section was incubated with ab315023 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with ab315023 at 1/50 (10.14 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing nuclear staining in RAW 264.7 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse hepatocellular carcinoma tissue labeling Transaldolase 1 with ab315023 at 1/8000 (0.063 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse hepatocellular carcinoma. The section was incubated with ab315023 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Flow Cytometry (Intracellular) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with ab315023 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • IP

Supplier Data

Immunoprecipitation - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Transaldolase 1 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab315023 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315023 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate

Lane 2 : ab315023 IP in C6 (rat glial tumor glial cell) whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315023 in C6 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Transaldolase 1 antibody [EPR28140-51] (<a href='/en-us/products/primary-antibodies/transaldolase-1-antibody-epr28140-51-ab315023'>ab315023</a>) at 1/30 dilution

All lanes:

C6 (rat glial tumor glial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

Immunoprecipitation - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • IP

Supplier Data

Immunoprecipitation - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Transaldolase 1 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab315023 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315023 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lane 2 : ab315023 IP in RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315023 in RAW 264.7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Transaldolase 1 antibody [EPR28140-51] (<a href='/en-us/products/primary-antibodies/transaldolase-1-antibody-epr28140-51-ab315023'>ab315023</a>) at 1/30 dilution

All lanes:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

Western blot - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • WB

Supplier Data

Western blot - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Transaldolase 1 antibody [EPR28140-51] (<a href='/en-us/products/primary-antibodies/transaldolase-1-antibody-epr28140-51-ab315023'>ab315023</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HEK-293 transfected with siRNA specifically targeting TALDO1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 3.25s

Western blot - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • WB

Supplier Data

Western blot - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Transaldolase 1 antibody [EPR28140-51] (<a href='/en-us/products/primary-antibodies/transaldolase-1-antibody-epr28140-51-ab315023'>ab315023</a>) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate whole cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate whole cell lysate at 20 µg

Lane 5:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate whole cell lysate at 20 µg

Lane 6:

C6 (rat glial tumor glial cell) whole cell lysatewhole cell lysate at 20 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate whole cell lysate at 20 µg

Lane 8:

C2C12 (mouse myoblast) whole cell lysate whole cell lysate at 20 µg

Lane 9:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysatewhole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa

false

Exposure time: 3.25s

Western blot - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)
  • WB

Supplier Data

Western blot - Anti-Transaldolase 1 antibody [EPR28140-51] - BSA and Azide free (AB315024)

This data was developed using ab315023, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Transaldolase 1 antibody [EPR28140-51] (<a href='/en-us/products/primary-antibodies/transaldolase-1-antibody-epr28140-51-ab315023'>ab315023</a>) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human liver cancer tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse colon cancer tissue lysate at 20 µg

Lane 5:

Mouse breast cancer tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Secondary

Lanes 1 - 2:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 3 - 6:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa

false

Exposure time: 3.25s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28140-51

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Primary Antibodies

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Anti-Transketolase antibody [7H1AA1]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody [7H1AA1] (AB112997)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab315024 is the carrier-free version of ab315023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transaldolase 1 also known as TALDO1 or TAL-H is an enzyme that plays an important role in the pentose phosphate pathway by catalyzing the transfer of a three-carbon dihydroxyacetone unit from sedoheptulose 7-phosphate to glyceraldehyde 3-phosphate producing erythrose 4-phosphate and fructose 6-phosphate. The molecular mass of Transaldolase 1 is approximately 37 kDa. It is expressed in various tissues within the body with higher expression levels observed in the liver and adipose tissues.
Biological function summary

Transaldolase 1 regulates the balance between the oxidative and non-oxidative branches of the pentose phosphate pathway essential for nucleotide biosynthesis and maintaining cellular redox status. It functions independently not as part of a larger enzyme complex. The activity of this enzyme affects cellular metabolic functions impacting the synthesis of nucleotides and pentose sugars which are necessary for cell growth and division.

Pathways

Transaldolase 1 is integral to the pentose phosphate pathway also known as the hexose monophosphate shunt. This pathway is important for providing ribose-5-phosphate for nucleotide synthesis and NADPH for reductive biosynthesis and cellular antioxidant systems. It links with glycolysis through shared intermediates such as fructose 6-phosphate connecting with enzymes like glucose-6-phosphate dehydrogenase. This relationship allows cells to adjust their metabolic flux according to physiological needs.

Transaldolase 1 mutations are associated with transaldolase deficiency a rare metabolic disorder that can lead to liver dysfunction and immunodeficiency. This deficiency affects the cellular ability to balance nucleotide and lipid biosynthesis contributing to the disease phenotype. There is also a connection with oxidative stress-related disorders as Transaldolase 1 indirectly influences reactive oxygen species via its role in NADPH production which ties it to proteins involved in oxidative stress responses like NADPH oxidase.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway. Catalyzes the reversible conversion of sedheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and beta-D-fructose 6-phosphate (PubMed : 18687684, PubMed : 8955144). Not only acts as a pentose phosphate pathway enzyme, but also affects other metabolite pathways by altering its subcellular localization between the nucleus and the cytoplasm (By similarity).
See full target information TALDO1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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