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Rabbit Recombinant Monoclonal Transcription factor AP-2-alpha antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 27 publications.


Images

Immunoprecipitation - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (AB108311), expandable thumbnail
  • Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (AB108311), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (AB108311), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (AB108311), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (AB108311), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Expected
Rat
Tested
Expected
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.

Alternative names

AP2TF, TFAP2, TFAP2A, Transcription factor AP-2-alpha, AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2

Recommended products

Rabbit Recombinant Monoclonal Transcription factor AP-2-alpha antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 27 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2688(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Transcription factor AP-2-alpha also known as TFAP2A or AP-2 is a protein acting as an important regulator in cellular processes. It weighs approximately 52 kDa. TFAP2A is known for its role in binding specific DNA sequences to regulate target gene transcription. It expresses highly in neural crest-derived tissues as well as in the placenta breast and skin. This transcription factor plays an essential role in embryonic development cell proliferation and differentiation.

Biological function summary

TFAP2A contributes significantly to the regulation of gene expression involved in essential developmental and cellular processes. It acts as part of a complex with other transcription factors allowing it to coordinate the expression of a variety of genes linked to cell growth and apoptosis. AP-2 also regulates genes involved in neural development and skin morphogenesis. Its interaction with other factors such as AP-2γ and AP-2β enhances its functionality indicating a complex mechanism of action.

Pathways

TFAP2A engages in critical signalling circuits such as the MAPK and Wnt pathways which are essential for cell cycle regulation and embryonic development. Within these pathways related proteins like AP-2γ and AP-594 interact with TFAP2A further illustrating its role in signal transduction. These relationships highlight the importance of TFAP2A in orchestrating cellular responses to growth signals and environmental cues.

Associated diseases and disorders

TFAP2A has links to a range of pathological conditions including cancer and neural crest-related disorders. In melanoma for example TFAP2A's regulatory role affects genes associated with tumor growth and metastasis. The protein's interaction with other transcription factors like AP-647 may influence cancer progression and therapy response. Additionally the dysregulation of TFAP2A affects neural crest development potentially leading to congenital disorders. Understanding these interactions assists in unraveling the molecular basis of such diseases and informs therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311), expandable thumbnail

    Immunoprecipitation - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    Purified ab108311 at 1/20 dilution (0.5µg) immunoprecipitating Transcription factor AP-2-alpha in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab108311 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab108311 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/10,000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 48 kDa

    All lanes: Immunoprecipitation - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    Predicted band size: 48 kDa

  • Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311), expandable thumbnail

    Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    All lanes: Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

    Lane 3: Mouse skin lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa

  • Flow Cytometry (Intracellular) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    Intracellular Flow Cytometry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    Immunocytochemistry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/50 dilution (3.4 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat breast tissue sections labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/100 dilution (1.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse breast tissue sections labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/100 dilution (1.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/100 dilution (1.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311), expandable thumbnail

    Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    Lanes 1- 2: Merged signal (red and green). Green - ab108311 observed at 48 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab108311 was shown to react with Transcription factor AP-2-alpha in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line ab265122 (knockout cell lysate Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate ab257736) was used. Wild-type HeLa and TFAP2A knockout HeLa cell lysates were subjected to SDS-PAGE. ab108311 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: TFAP2A knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line ab265122)

    Performed under reducing conditions.

    Predicted band size: 122 kDa, 125 kDa, 32 kDa, 39 kDa, 40 kDa, 41 kDa, 42 kDa, 45 kDa, 48 kDa, 74 kDa, 77 kDa, 83 kDa, 97 kDa

    Observed band size: 100 kDa, 120 kDa, 125 kDa, 150 kDa, 31 kDa, 40 kDa, 41 kDa, 42 kDa, 45 kDa, 48 kDa, 50 kDa, 70 kDa, 74 kDa, 75 kDa, 77 kDa, 95 kDa

  • Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311), expandable thumbnail

    Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311)

    Lanes 1 - 2: Merged signal (red and green). Green - ab108311 observed at 48 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab108311 was shown to recognize Transcription factor AP-2-alpha in wild-type HAP1 cells as signal was lost at the expected MW in TFAP2A (Transcription factor AP-2-alpha) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TFAP2A (Transcription factor AP-2-alpha) knockout samples were subjected to SDS-PAGE. ab108311 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: TFAP2A (Transcription factor AP-2-alpha) knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 44 kDa, 48 kDa

    Observed band size: 44 kDa

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Product protocols

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