Anti-Transferrin antibody [EPR18240-29] - Serum Loading Control

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [EPR18240-29] - Serum Loading Control (AB278498)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 cells labelling Transferrin with ab278498 at 1/100 (5.52 μg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Hepa1-6 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR18240-29] - Serum Loading Control (AB278498)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (Mouse hepatoma epithelial cell) cells labelling Transferrin with ab278498 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• WB

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Western blot - Anti-Transferrin antibody [EPR18240-29] - Serum Loading Control (AB278498)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-Transferrin antibody [EPR18240-29] - Serum Loading Control (ab278498) at 1/1000 dilution

All lanes:

Mouse plasma at 20 µL

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 76 kDa

false

• WB

Supplier Data

Western blot - Anti-Transferrin antibody [EPR18240-29] - Serum Loading Control (AB278498)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 1-3 : 3.25 seconds; Lane 4 : 70 seconds

All lanes:

Western blot - Anti-Transferrin antibody [EPR18240-29] - Serum Loading Control (ab278498) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 77 kDa

Observed band size: 76 kDa

false