AB769

Anti-Transferrin antibody [HTF-14] - Serum Loading Control

Name: Anti-Transferrin antibody [HTF-14] - Serum Loading Control (ab769) | Abcam
Brand: Abcam Limited
SKU: AB769
Price: 400 USD
Availability: InStock
Rating: 1 (1 reviews)

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What is this?

(1 Review)

(13 Publications)

Mouse Monoclonal Transferrin antibody. Suitable for ICC, ELISA, WB, sELISA, IHC-P, RIA, IP, FuncS and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Native Full Length Protein corresponding to Pig TF.

View Alternative Names

PRO1400, TF, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

3 Images

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [HTF-14] - Serum Loading Control (AB769)

Immunohistochemistry staining of human placenta (paraffin sections) using ab769 10 μg/ml

Supplier Data

Western blot - Anti-Transferrin antibody [HTF-14] - Serum Loading Control (AB769)

The sample (commercial human transferrin) (1μg/ml in 1x PBS) was mixed with 4x loading buffer (non reducing) or with Laemmli buffer (reducing) and warmed for 3 minutes.

Volume of sample : 5; 3; 1 μl of sample + 3; 2; 2 μl of buffer

SDS Page : 12% AA-gel, U=180V, 90 minutes, volume of loading sample : 8; 5;3 μl

Marker – 3 μl

WB : Nitrocelulose membrane (8x5.5 cm), I=35mA, 90minutes

Detection :

Blocking solution : 1xPBS/5% BSA, 4°C, overnight

Primary Ab : membrane was incubated in 5 ml DMEM/10%FTS + 10 μl HTF-14 (primary Ab) (60 minutes, room temperature)

Washing solution : 1x PBS/0.1% Tween

Secondary Ab : IRDye^® 800CW Goat anti-mouse IgG1-specific, dilution 1/5000, diluting solution – 5% BSA/1x PBS, incubation for 60 minutes, low temperature.

Detection : Li-COR odyssey

No reducing agent should be added to the sample buffer. The sample can be denatured and processed by common Western blotting protocols, but the disulfide bonds must not be reduced.

All lanes:

Western blot - Anti-Transferrin antibody [HTF-14] - Serum Loading Control (ab769)

Lane 1:

Human Transferrin – 8 µl (reducing conditions) at 5 µg

Lane 2:

HumanTransferrin – 5 µl (reducing conditions) at 3 µg

Lane 3:

Human Transferrin – 3 µl (reducing conditions) at 1 µg

Lane 4:

Human Transferrin – 3 µl (non reducing conditions) at 1 µg

Lane 5:

Human Transferrin – 5 µl (non reducing conditions) at 3 µg

Lane 6:

Human Transferrin - 8 µl (non reducing conditions) at 5 µg

Predicted band size: 77 kDa

false

sELISA

Unknown

Sandwich ELISA - Anti-Transferrin antibody [HTF-14] - Serum Loading Control (AB769)

Standard curve for Transferrin (Analyte : ab83560); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1μg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HTF-14

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

WB, sELISA, ELISA, IHC-P, FuncS, IP, RIA, ICC

applications

Immunogen

Native Full Length Protein corresponding to Pig TF.

P09571

Specificity

This antibody recognizes an epitope located in the N-terminal domain of human transferrin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Works best under non reducing conditions.", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Capture at 1μg/ml with <a href='/en-us/products/unavailable/transferrin-antibody-ab9538'>ab9538</a> as Detection.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "Effectively blocks the transferrin-transferrin receptor interactions." }, "Carp": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "Works best under non reducing conditions.", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Capture at 1μg/ml with <a href='/en-us/products/unavailable/transferrin-antibody-ab9538'>ab9538</a> as Detection.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "RIA-species-checked": "notRecommended", "RIA-species-dilution-info": "", "RIA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "Effectively blocks the transferrin-transferrin receptor interactions." }, "Cow": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "Works best under non reducing conditions.", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Capture at 1μg/ml with <a href='/en-us/products/unavailable/transferrin-antibody-ab9538'>ab9538</a> as Detection.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "RIA-species-checked": "notRecommended", "RIA-species-dilution-info": "", "RIA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "Effectively blocks the transferrin-transferrin receptor interactions." }, "Dog": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "Works best under non reducing conditions.", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Capture at 1μg/ml with <a href='/en-us/products/unavailable/transferrin-antibody-ab9538'>ab9538</a> as Detection.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "RIA-species-checked": "notRecommended", "RIA-species-dilution-info": "", "RIA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "Effectively blocks the transferrin-transferrin receptor interactions." }, "Horse": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "Works best under non reducing conditions.", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Capture at 1μg/ml with <a href='/en-us/products/unavailable/transferrin-antibody-ab9538'>ab9538</a> as Detection.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "RIA-species-checked": "notRecommended", "RIA-species-dilution-info": "", "RIA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "Effectively blocks the transferrin-transferrin receptor interactions." }, "Pig": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "RIA-species-checked": "notRecommended", "RIA-species-dilution-info": "", "RIA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "" }, "Rabbit": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "RIA-species-checked": "notRecommended", "RIA-species-dilution-info": "", "RIA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "" }, "Sheep": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "Works best under non reducing conditions.", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Capture at 1μg/ml with <a href='/en-us/products/unavailable/transferrin-antibody-ab9538'>ab9538</a> as Detection.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "RIA-species-checked": "notRecommended", "RIA-species-dilution-info": "", "RIA-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "Effectively blocks the transferrin-transferrin receptor interactions." } } }

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purified from ascites by sequential precipitation methods. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.

Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.

See full target information TF

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112647 PubMed37330908

2023

Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lixiang Sun,Xiaowei Zhang,Shuai Wu,Youxi Liu,Christian F Guerrero-Juarez,Wenjie Liu,Jinwen Huang,Qian Yao,Meimei Yin,Jiacheng Li,Raul Ramos,Yanhang Liao,Rundong Wu,Tian Xia,Xinyuan Zhang,Yichun Yang,Fengwu Li,Shujun Heng,Wenlu Zhang,Minggang Yang,Chi-Meng Tzeng,Chao Ji,Maksim V Plikus,Richard L Gallo,Ling-Juan Zhang

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 41:219-235 PubMed32151223

2020

Cerebral angiogenesis ameliorates pathological disorders in -deficient mice with small-vessel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Jiang,Kristin Müller,Mahtab A Khan,Julian C Assmann,Josephine Lampe,Knut Kilau,Marius Richter,Maximilian Kleint,Dirk A Ridder,Norbert Hübner,Marc Schmidt-Supprian,Jan Wenzel,Markus Schwaninger

Immunity 50:121-136.e5 PubMed30594464

2018

Age-Related Loss of Innate Immune Antimicrobial Function of Dermal Fat Is Mediated by Transforming Growth Factor Beta.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Juan Zhang,Stella Xiang Chen,Christian F Guerrero-Juarez,Fengwu Li,Yun Tong,Yuqiong Liang,Marc Liggins,Xu Chen,Hao Chen,Min Li,Tissa Hata,Ye Zheng,Maksim V Plikus,Richard L Gallo

EBioMedicine 36:29-38 PubMed30243490

2018

Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Lu,Tingting Yuan,Yingge Wang,Ting Zhang,Yuguo Yuan,Daijin Wu,Minya Zhou,Zhengyi He,Yaoyao Lu,Yajie Chen,Jianglin Fan,Jingyan Liang,Yong Cheng

Cancer cell 33:874-889.e7 PubMed29681511

2018

A Glial Signature and Wnt7 Signaling Regulate Glioma-Vascular Interactions and Tumor Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Amelie Griveau,Giorgio Seano,Samuel J Shelton,Robert Kupp,Arman Jahangiri,Kirsten Obernier,Shanmugarajan Krishnan,Olle R Lindberg,Tracy J Yuen,An-Chi Tien,Jennifer K Sabo,Nancy Wang,Ivy Chen,Jonas Kloepper,Louis Larrouquere,Mitrajit Ghosh,Itay Tirosh,Emmanuelle Huillard,Arturo Alvarez-Buylla,Michael C Oldham,Anders I Persson,William A Weiss,Tracy T Batchelor,Anat Stemmer-Rachamimov,Mario L Suvà,Joanna J Phillips,Manish K Aghi,Shwetal Mehta,Rakesh K Jain,David H Rowitch

Nanoscale 10:5474-5481 PubMed29511756

2018

Label-free in-flow detection of receptor recognition motifs on the biomolecular corona of nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

M Gianneli,E Polo,H Lopez,V Castagnola,T Aastrup,K A Dawson

Nature communications 7:13475 PubMed27845346

2016

In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry.

Applications

Flow Cyt

Species

Human

Maria Cristina Lo Giudice,Luciana M Herda,Ester Polo,Kenneth A Dawson

Oncology letters 10:1848-1852 PubMed26622763

2015

Identification and functional study of osteosarcoma metastasis marker genes.

Applications

Species

Human

Rui Shi,Juan Li,Fan Tang,Y I Luo,Chong-Qi Tu

Nature nanotechnology 8:137-43 PubMed23334168

2013

Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Salvati,Andrzej S Pitek,Marco P Monopoli,Kanlaya Prapainop,Francesca Baldelli Bombelli,Delyan R Hristov,Philip M Kelly,Christoffer Åberg,Eugene Mahon,Kenneth A Dawson

PloS one 7:e40685 PubMed22829881

2012

Transferrin coated nanoparticles: study of the bionano interface in human plasma.

Applications

Dot

Species

Unspecified reactive species

Andrzej S Pitek,David O'Connell,Eugene Mahon,Marco P Monopoli,Francesca Baldelli Bombelli,Kenneth A Dawson

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Transferrin antibody [HTF-14] - Serum Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [HTF-14] - Serum Loading Control (AB769)

Western blot - Anti-Transferrin antibody [HTF-14] - Serum Loading Control (AB769)

Sandwich ELISA - Anti-Transferrin antibody [HTF-14] - Serum Loading Control (AB769)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise