Rabbit Recombinant Multiclonal Transferrin antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--immunocytochemistry-immunofluorescence-img427516.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296)")
![Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--western-blot-img427437.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296)")
![Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--western-blot-img427438.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296)")
![Flow Cytometry (Intracellular) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--flow-cytometry-intracellular-img427525.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427504.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (AB316296)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | Flow Cyt (Intra) | IP | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6:ESAG7 and extract its iron for its own use.
Alternative names
PRO1400, TF, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin
Recommended products
Rabbit Recombinant Multiclonal Transferrin antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1092
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316296 is the carrirer-free version of Anti-Transferrin antibody [RM1092] ab316295.
This product is a recombinant multiclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.
- Biological function summary
Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.
- Pathways
Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.
- Associated diseases and disorders
Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--immunocytochemistry-immunofluorescence-img427516.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)")
Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)
This data was developed using Anti-Transferrin antibody [RM1092] ab316295, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Transferrin with Anti-Transferrin antibody [RM1092] ab316295 at 1/500 (0.98 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in HepG2 cell line.
Negative control: K-562
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--western-blot-img427437.jpg "Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)")
Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)
This data was developed using Anti-Transferrin antibody [RM1092] ab316295, the same antibody clone in a different buffer formulation.
Negative control: K-562.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Transferrin antibody [RM1092] (Anti-Transferrin antibody [RM1092] ab316295) at 1/1000 dilution
Lane 1: HepG2(human hepatocellar carcinoma epithelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 77 kDa, 36 kDa
Exposure time: 15s
![Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--western-blot-img427438.jpg "Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)")
Western blot - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)
This data was developed using Anti-Transferrin antibody [RM1092] ab316295, the same antibody clone in a different buffer formulation.
Transferrin might be easily degraded. The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15271890).
All lanes: Western blot - Anti-Transferrin antibody [RM1092] (Anti-Transferrin antibody [RM1092] ab316295) at 1/1000 dilution
Lane 1: Human plasma tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 77 kDa
Exposure time: 26s
![Flow Cytometry (Intracellular) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--flow-cytometry-intracellular-img427525.jpg "Flow Cytometry (Intracellular) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)")
Flow Cytometry (Intracellular) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)
This data was developed using Anti-Transferrin antibody [RM1092] ab316295, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling Transferrin with Anti-Transferrin antibody [RM1092] ab316295 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: K-562. For Anti-Transferrin antibody [RM1092] ab316295, positive staining occurred in HepG2 cells and no staining in K-562 cells. The control batch (Anti-Transferrin antibody ab82411) showed similar staining pattern. In addition, Anti-Transferrin antibody [RM1092] ab316295 showed stronger staining than Anti-Transferrin antibody ab82411 in HepG2 cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427504.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)
This data was developed using Anti-Transferrin antibody [RM1092] ab316295, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Transferrin with Anti-Transferrin antibody [RM1092] ab316295 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human placenta.
The section was incubated with Anti-Transferrin antibody [RM1092] ab316295 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427503.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)
This data was developed using Anti-Transferrin antibody [RM1092] ab316295, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Transferrin with Anti-Transferrin antibody [RM1092] ab316295 at 1/100 (4.93 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human liver.
The section was incubated with Anti-Transferrin antibody [RM1092] ab316295 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunoprecipitation - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--immunoprecipitation-img427447.jpg "Immunoprecipitation - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)")
Immunoprecipitation - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)
This data was developed using Anti-Transferrin antibody [RM1092] ab316295, the same antibody clone in a different buffer formulation.
Transferrin was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with Anti-Transferrin antibody [RM1092] ab316295 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Transferrin antibody [RM1092] ab316295 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2: Anti-Transferrin antibody [RM1092] ab316295 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Transferrin antibody [RM1092] ab316295 in HepG2 whole cell lysateAll lanes: Immunoprecipitation - Anti-Transferrin antibody [RM1092] (Anti-Transferrin antibody [RM1092] ab316295) at 1/30 dilution
All lanes: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427503.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)")
![Immunoprecipitation - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296), expandable thumbnail](https://content.abcam.com/products/images/transferrin-antibody-rm1092-bsa-and-azide-free-ab316296--immunoprecipitation-img427447.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Transferrin antibody [RM1092] - BSA and Azide free (ab316296)")
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