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AB318998

Anti-Transferrin antibody [RM2060] - BSA and Azide free

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Rabbit Recombinant Multiclonal Transferrin antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF and reacts with Human, Mouse samples.

View Alternative Names

Trf, Tf, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

14 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human non-perfused fixed skeletal muscle tissue labeling Transferrin with ab318997 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in human non-perfused fixed skeletal muscle.
The section was incubated with ab318997 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human non-perfused fixed thyroid carcinoma tissue labeling Transferrin with ab318997 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in plasma of human non-perfused fixed thyroid carcinoma.
The section was incubated with ab318997 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Transferrin with ab318997 at 1/100 (5.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

Confocal image showing cytoplasmic staining in HepG2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression : K-562
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human non-perfused fixed colon tissue labeling Transferrin with ab318997 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in plasma of human non-perfused fixed colon.
The section was incubated with ab318997 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) (Right) / K-562 (human chronic myelogenous leukemia lymphoblast) (Left) cells labelling Transferrin with ab318997 at 1/500 dilution (0.1ug )/ Magenta compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : K-562.

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling Transferrin with ab318997 at 1/100 (5.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

Confocal image showing cytoplasmic staining in Hepa1-6 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression : C2C12
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Immunoprecipitation - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • IP

Supplier Data

Immunoprecipitation - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Transferrin was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab318997 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318997 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : ab318997 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318997 in HepG2 whole cell lysate

All lanes:

Immunoprecipitation - Anti-Transferrin antibody [RM2060] - Serum Loading Control (<a href='/en-us/products/primary-antibodies/transferrin-antibody-rm2060-serum-loading-control-ab318997'>ab318997</a>) at 1/30 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Mouse perfused fixed cardiac muscle. (B) Mouse non-perfused fixed cardiac muscle. tissue labeling Transferrin with ab318997 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nearly no staining in (A) mouse perfused fixed cardiac muscle; positive staining in plasma of (B) mouse non-perfused fixed cardiac muscle.
The section was incubated with ab318997 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Mouse perfused fixed liver. (B) Mouse non-perfused fixed liver. tissue labeling Transferrin with ab318997 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in both (A) perfused fixed and (B) non-perfused fixed mouse liver.
The section was incubated with ab318997 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse perfused fixed skeletal muscle tissue labeling Transferrin with ab318997 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : Nearly no staining in mouse perfused fixed skeletal muscle.
The section was incubated with ab318997 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunoprecipitation - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • IP

Supplier Data

Immunoprecipitation - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Transferrin was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with ab318997 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318997 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse liver tissue lysate
Lane 2 : ab318997 IP in Mouse liver tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318997 in mouse liver tissue lysate

All lanes:

Immunoprecipitation - Anti-Transferrin antibody [RM2060] - Serum Loading Control (<a href='/en-us/products/primary-antibodies/transferrin-antibody-rm2060-serum-loading-control-ab318997'>ab318997</a>) at 1/30 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

Western blot - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • WB

Supplier Data

Western blot - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Exposure time : Lane 1 : 114 seconds, lanes 2-3 : 15 seconds

All lanes:

Western blot - Anti-Transferrin antibody [RM2060] - Serum Loading Control (<a href='/en-us/products/primary-antibodies/transferrin-antibody-rm2060-serum-loading-control-ab318997'>ab318997</a>) at 1/1000 dilution

Lane 1:

Human plasma lysate at 20 µg with NFDM/TBST

Lane 2:

Mouse plasma lysate at 20 µg with NFDM/TBST

Lane 3:

Mouse serum lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 76 kDa

false

Western blot - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • WB

Supplier Data

Western blot - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Low expression : skeletal muscle (PMID : 3558394).

The identity of the lower MW band at approximately 73 kDa (in lane 5) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Transferrin antibody [RM2060] - Serum Loading Control (<a href='/en-us/products/primary-antibodies/transferrin-antibody-rm2060-serum-loading-control-ab318997'>ab318997</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg with NFDM/TBST

Lane 2:

Human breast tissue lysate at 20 µg with NFDM/TBST

Lane 3:

Human liver tissue lysate at 20 µg with NFDM/TBST

Lane 4:

Human testis tissue lysate at 20 µg with NFDM/TBST

Lane 5:

Human skeletal muscle tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 76 kDa,36 kDa

false

Exposure time: 15s

Western blot - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)
  • WB

Supplier Data

Western blot - Anti-Transferrin antibody [RM2060] - BSA and Azide free (AB318998)

This data was developed using ab318997, the same antibody clone in a different buffer formulation.

Low expression : skeletal muscle (PMID : 3558394).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Transferrin antibody [RM2060] - Serum Loading Control (<a href='/en-us/products/primary-antibodies/transferrin-antibody-rm2060-serum-loading-control-ab318997'>ab318997</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg with NFDM/TBST

Lane 2:

Mouse heart tissue lysate at 20 µg with NFDM/TBST

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 76 kDa,36 kDa

false

Exposure time: 114s

  • Unconjugated

    Anti-Transferrin antibody [RM2060] - Serum Loading Control

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM2060

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Unsuitable for mouse FC-intra. Not recommended for use in WB with cell lysates. Band at correct Mw only detected in cell lysates after enrichment of Transferrin by IP

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab318998 is the carrier-free version of ab318997.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.
Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
See full target information Tf

Additional targets

TF
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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