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Mouse Recombinant Monoclonal Transferrin Receptor antibody. Suitable for WB, ICC/IF and reacts with Human samples.

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Images

Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (AB300657), expandable thumbnail
  • Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (AB300657), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [2/Transferrin] (AB300657), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Not recommended
Not recommended
Rat
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738). Mediates uptake of NICOL1 into fibroblasts where it may regulate extracellular matrix production (By similarity). (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

Additional Targets

TFRC phospho S24, TFRC phospho S24

Alternative names

CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC

Recommended products

Mouse Recombinant Monoclonal Transferrin Receptor antibody. Suitable for WB, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
2/Transferrin H1L1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.

Biological function summary

TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.

Pathways

The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.

Associated diseases and disorders

Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657), expandable thumbnail

    Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657)

    All lanes: Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657) at 1/1000 dilution

    Lane 1: Human placenta tissue lysate at 20 µg

    Lane 2: Human thymus tissue lysate at 20 µg

    Lane 3: SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 5: LoVo (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 6: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

    Observed band size: 84 kDa

  • Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657), expandable thumbnail

    Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657)

    All lanes: Western blot - Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657) at 1/1000 dilution

    Lane 1: Human placenta tissue lysate at 20 µg

    Lane 2: HeLa (human epithelial cell line from cervical adenocarcinoma), whole cell lysate at 20 µg

    Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

    Lane 4: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 5: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 20 µg

    Lane 6: SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

    Observed band size: 84 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [2/Transferrin] (ab300657)

    Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling Transferrin receptor with ab300657 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in K-562 cell line is observed. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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