AB216665

Anti-Transferrin Receptor antibody

Name: Anti-Transferrin Receptor antibody (ab216665) | Abcam
Brand: Abcam Limited
SKU: AB216665
Price: 515 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal Transferrin Receptor antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TFRC aa 600-700 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC

3 Images

Flow Cytometry - Anti-Transferrin Receptor antibody (AB216665)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Transferrin Receptor antibody (AB216665)

Flow cytometry analysis of Raji (Human Burkitt's lymphoma cell line) cells labeling Transferrin Receptor (green, right histogram), using ab216665 at a 1/100 dilution, and negative control cells (blue). As secondary antibody PE-conjugated was used. Secondary only (light blue) and isotope control (orange).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Transferrin Receptor antibody (AB216665)

Flow cytometric overlay histogram showing K562 labelling Transferring Receptor with Rabbit Anti-CD71 antibody (ab216665) at 1μg /10^6 cells dilution (Green line). Isotype control antibody (Orange line) was Rabbit IgG. Goat anti-rabbit IgG-FITC, at 0.5 ug/test dilution was used as the secondary antibody.

The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Supplier Data

Western blot - Anti-Transferrin Receptor antibody (AB216665)

All lanes:

Western blot - Anti-Transferrin Receptor antibody (ab216665) at 1/1000 dilution

Lane 1:

HeLa cell (Human) Lysate at 40 µg

Lane 2:

Ht1080 cell (Human) Lysate at 40 µg

Lane 3:

U937 cell (Human) Lysate at 40 µg

Secondary

All lanes:

IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

Predicted band size: 84 kDa

Observed band size: 95-100 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human TFRC aa 600-700 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P02786

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20 - 1/100", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.

Biological function summary

TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.

Pathways

The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.

Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene TFRC is involved in the cellular uptake of iron via receptor-mediated endocytosis, where the transferrin receptor, bound to its ligand, is internalized into specialized endosomes. Following endosomal acidification, iron is released, and the apotransferrin-receptor complex is recycled to the cell surface, where a return to neutral pH results in the loss of apotransferrin's affinity for its receptor. The hereditary hemochromatosis protein HFE competes with transferrin for binding at an overlapping C-terminal site. TFRC positively regulates T and B cell proliferation through iron uptake. It functions as a lipid sensor influencing mitochondrial fusion by modulating the JNK pathway activity. Low dietary levels of stearate promote JNK pathway activation, leading to HUWE1-mediated ubiquitination and degradation of the mitofusin MFN2, inhibiting mitochondrial fusion. High dietary stearate levels result in TFRC stearoylation, which inhibits JNK pathway activation and MFN2 degradation. Additionally, TFRC acts as a receptor for new-world arenaviruses, including Guanarito, Junin, and Machupo viruses. This supplementary information is collated from multiple sources and compiled automatically.

See full target information TFRC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 24:726 PubMed36340606

2022

ASPP2 promotes cell apoptosis in cervical cancer through inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Feiyun Jiang,Ganxia Bian,Jiehua Li

iScience 25:104813 PubMed35982785

2022

A microengineered Brain-Chip to model neuroinflammation in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Iosif Pediaditakis,Konstantia R Kodella,Dimitris V Manatakis,Christopher Y Le,Sonalee Barthakur,Alexander Sorets,Achille Gravanis,Lorna Ewart,Lee L Rubin,Elias S Manolakos,Christopher D Hinojosa,Katia Karalis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Transferrin Receptor antibody

Flow Cytometry - Anti-Transferrin Receptor antibody (AB216665)

Flow Cytometry - Anti-Transferrin Receptor antibody (AB216665)

Western blot - Anti-Transferrin Receptor antibody (AB216665)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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