Anti-Transferrin Receptor antibody

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (AB84036)

ab84036 staining Transferrin receptor protein 1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab84036 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• WB

Unknown

Western blot - Anti-Transferrin Receptor antibody (AB84036)

Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Transferrin Receptor antibody (ab84036) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate at 10 µg

Lane 3:

U-2 OS (Human osteosarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 84 kDa

Observed band size: 37 kDa,98 kDa

true

Exposure time: 4min

• WB

Unknown

Western blot - Anti-Transferrin Receptor antibody (AB84036)

Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Transferrin Receptor antibody (ab84036) at 1 µg/mL

All lanes:

Mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 84 kDa

Observed band size: 100 kDa,50 kDa

true

Exposure time: 8min