Anti-Transferrin receptor rabbit polyclonal antibody that is used to detect TFRC in Western Blot and ICC/IF. Suitable for Human and Mouse samples.
- Cited in over 200 publications
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Publications
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- Journal of cell science 136:2023Regulated dynamic subcellular GLUT4 localization revealed by proximal proteome mapping in human muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnuttoma Ray,Jennifer Wen,Lucie Yammine,Jeff Culver,Isabella Supardi Parida,Jeonifer Garren,Liang Xue,Katherine Hales,Qing Xiang,Morris J Birnbaum,Bei B Zhang,Mara Monetti,Timothy E McGrawPubMed 38126809
- Cells 12:2023Anticancer Effects of 6-Gingerol through Downregulating Iron Transport and PD-L1 Expression in Non-Small Cell Lung Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Young Kang,Sanghyeon Park,Kyoung Seob Song,Se Won Bae,Jeong-Sang Lee,Kyoung-Jin Jang,Yeong-Min ParkPubMed 37998363
- Molecular neurobiology 61:2313-23352023Sevoflurane Exposure Induces Neuronal Cell Ferroptosis Initiated by Increase of Intracellular Hydrogen Peroxide in the Developing Brain via ER Stress ATF3 Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiheng Kang,Meihua Piao,Nan Liu,Wanping Gu,Chunsheng FengPubMed 37874483
- iScience 26:1082702023Fucoxanthin ameliorates traumatic brain injury by suppressing the blood-brain barrier disruption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Zhang,Zhigang Hu,Wanshan Bai,Yaonan Peng,Yixing Lin,Zixiang CongPubMed 37965135
- Cellular and molecular neurobiology 43:4173-41872023A Perfused In Vitro Human iPSC-Derived Blood-Brain Barrier Faithfully Mimics Transferrin Receptor-Mediated Transcytosis of Therapeutic Antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFloriana Burgio,Carine Gaiser,Kevin Brady,Viviana Gatta,Reiner Class,Ramona Schrage,Laura Suter-DickPubMed 37698826
- iScience 26:1078192023Dynamic association of the intramembrane proteases SPPL2a/b and their substrates with tetraspanin-enriched microdomains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadja Leinung,Torben Mentrup,Mehul Patel,Tom Gallagher,Bernd SchröderPubMed 37736044
- Scientific reports 13:127942023Hepcidin deficiency in mice impairs white adipose tissue browning possibly due to a defect in de novo adipogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean-Christophe Deschemin,Céline Ransy,Frédéric Bouillaud,Soonkyu Chung,Bruno Galy,Carole Peyssonnaux,Sophie VaulontPubMed 37550331
- International journal of molecular sciences 24:2023Induction of Hepcidin Expression in the Renal Cortex of Sickle Cell Disease Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsrar Ahmad,Namita Kumari,Nowah Afangbedji,Sergei Nekhai,Marina JerebtsovaPubMed 37445980
- Antioxidants & redox signaling 40:492-5092023Dapagliflozin Alleviates Diabetic Kidney Disease Hypoxia Inducible Factor 1α/Heme Oxygenase 1-Mediated Ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Hui Wang,Dong-Yuan Chang,Ming-Hui Zhao,Min ChenPubMed 37276148
- International journal of molecular sciences 24:2023The E3 Ubiquitin Ligase TRIM21 Regulates Basal Levels of PDGFRβ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNiki Sarri,Natalia Papadopoulos,Johan Lennartsson,Carl-Henrik HeldinPubMed 37175489
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Tested |
| Dog | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738). Mediates uptake of NICOL1 into fibroblasts where it may regulate extracellular matrix production (By similarity). (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Alternative names
CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC
Recommended products
Anti-Transferrin receptor rabbit polyclonal antibody that is used to detect TFRC in Western Blot and ICC/IF. Suitable for Human and Mouse samples.
- Cited in over 200 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Transferrin Receptor antibody (ab84036) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in human, mouse samples.
Anti-Transferrin Receptor antibody (ab84036) specifically detects Transferrin Receptor (UniProt ID: P02786; Molecular weight: 85kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Transferrin Receptor antibody (ab84036) has high sensitivity and specificity.
Anti-Transferrin Receptor antibody (ab84036) has been cited over 200 times in peer reviewed journals and is trusted by the scientific community.
Anti-Transferrin Receptor antibody (ab84036) has 20 independent reviews from customers.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab84036)
ab84036 staining Transferrin receptor protein 1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab84036 at 5?g/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Transferrin Receptor antibody (ab84036)
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-Transferrin Receptor antibody (ab84036) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) whole cell lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) whole cell lysate at 10 µg
Lane 3: U-2 OS (Human osteosarcoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 37 kDa, 98 kDa
Exposure time: 4min
Western blot - Anti-Transferrin Receptor antibody (ab84036)
Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-Transferrin Receptor antibody (ab84036) at 1 µg/mL
All lanes: Mouse spleen tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 100 kDa, 50 kDa
Exposure time: 8min
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