AB108985

Anti-Transferrin Receptor antibody [EPR4012]

Name: Anti-Transferrin Receptor antibody [EPR4012] (ab108985) | Abcam
Brand: Abcam Limited
SKU: AB108985
Price: 385 USD
Availability: InStock
Rating: 4 (4 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(4 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal Transferrin Receptor antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC

2 Images

Flow Cytometry (Intracellular) - Anti-Transferrin Receptor antibody [EPR4012] (AB108985)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Transferrin Receptor antibody [EPR4012] (AB108985)

Overlay histogram showing Jurkat cells stained with ab108985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108985, 1/100) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Western blot - Anti-Transferrin Receptor antibody [EPR4012] (AB108985)

All lanes:

Western blot - Anti-Transferrin Receptor antibody [EPR4012] (ab108985) at 1/1000 dilution

Lane 1:

Human placenta cell lysate at 10 µg

Lane 2:

TF-1 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 84 kDa

Observed band size: 90 kDa

false

Carrier free

Anti-Transferrin Receptor antibody [EPR4012] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Transferrin Receptor antibody [EPR4012]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Transferrin Receptor antibody [EPR4012]
660 APC

APC Anti-Transferrin Receptor antibody [EPR4012]
578 PE

PE Anti-Transferrin Receptor antibody [EPR4012]
Carrier free

Anti-Transferrin Receptor antibody [EPR4012] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4012

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.

Biological function summary

TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.

Pathways

The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.

Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene TFRC is involved in the cellular uptake of iron via receptor-mediated endocytosis, where the transferrin receptor, bound to its ligand, is internalized into specialized endosomes. Following endosomal acidification, iron is released, and the apotransferrin-receptor complex is recycled to the cell surface, where a return to neutral pH results in the loss of apotransferrin's affinity for its receptor. The hereditary hemochromatosis protein HFE competes with transferrin for binding at an overlapping C-terminal site. TFRC positively regulates T and B cell proliferation through iron uptake. It functions as a lipid sensor influencing mitochondrial fusion by modulating the JNK pathway activity. Low dietary levels of stearate promote JNK pathway activation, leading to HUWE1-mediated ubiquitination and degradation of the mitofusin MFN2, inhibiting mitochondrial fusion. High dietary stearate levels result in TFRC stearoylation, which inhibits JNK pathway activation and MFN2 degradation. Additionally, TFRC acts as a receptor for new-world arenaviruses, including Guanarito, Junin, and Machupo viruses. This supplementary information is collated from multiple sources and compiled automatically.

See full target information TFRC

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of veterinary medical science 87:634-639 PubMed40254461

2025

A rare case of acute erythroid leukemia (AML M6-Er) with pleural metastasis and malignant pleural effusion in a cat.

Applications

Unspecified application

Species

Unspecified reactive species

Ga-Hyun Lim,Se-Hoon Kim,Min-Ok Ryu,Ju-Hyun An,Du-Min Go,Yeseul Yang,Dae-Yong Kim,Yongbaek Kim,Hwa-Young Youn,Kyoung-Won Seo

Pigment cell & melanoma research 35:328-341 PubMed35218147

2022

Altered expression of ferroptosis markers and iron metabolism reveals a potential role of ferroptosis in vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuyi Wu,Shanglin Jin,Yiwen Yang,Xiaoli Lu,Xiaoxi Dai,Zhongyi Xu,Chengfeng Zhang,Leihong Flora Xiang

Frontiers in immunology 12:796065 PubMed35003119

2021

Rubella Virus Infected Macrophages and Neutrophils Define Patterns of Granulomatous Inflammation in Inborn and Acquired Errors of Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Ludmila Perelygina,Raeesa Faisthalab,Emily Abernathy,Min-Hsin Chen,LiJuan Hao,Lionel Bercovitch,Diana K Bayer,Lenora M Noroski,Michael T Lam,Maria Pia Cicalese,Waleed Al-Herz,Arti Nanda,Joud Hajjar,Koen Vanden Driessche,Shari Schroven,Julie Leysen,Misha Rosenbach,Philipp Peters,Johannes Raedler,Michael H Albert,Roshini S Abraham,Hemalatha G Rangarjan,David Buchbinder,Lisa Kobrynski,Anne Pham-Huy,Julie Dhossche,Charlotte Cunningham Rundles,Anna K Meyer,Amy Theos,T Prescott Atkinson,Amy Musiek,Mehdi Adeli,Ute Derichs,Christoph Walz,Renate Krüger,Horst von Bernuth,Christoph Klein,Joseph Icenogle,Fabian Hauck,Kathleen E Sullivan

International journal of molecular sciences 22: PubMed34445629

2021

A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Zhang,Tao Bing,Luyao Shen,Le Feng,Xiangjun Liu,Dihua Shangguan

Blood 137:2090-2102 PubMed33529321

2021

Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Floriane Petit,Anthony Drecourt,Michaël Dussiot,Coralie Zangarelli,Olivier Hermine,Arnold Munnich,Agnès Rötig

Placenta 55:29-36 PubMed28623970

2017

Localization of the placental BCRP/ABCG2 transporter to lipid rafts: Role for cholesterol in mediating efflux activity.

Applications

Unspecified application

Species

Unspecified reactive species

John T Szilagyi,Anna M Vetrano,Jeffrey D Laskin,Lauren M Aleksunes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Transferrin Receptor antibody [EPR4012]

Flow Cytometry (Intracellular) - Anti-Transferrin Receptor antibody [EPR4012] (AB108985)

Western blot - Anti-Transferrin Receptor antibody [EPR4012] (AB108985)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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