Mouse Recombinant Monoclonal Transferrin Receptor antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 9 publications.
Images
![Western blot - Anti-Transferrin Receptor antibody [H68.4] (AB269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--western-blot-img82785.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Transferrin Receptor antibody [H68.4] (AB269513)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (AB269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113519.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (AB269513)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (AB269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17671.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (AB269513)")
![Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (AB269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--immunocytochemistry-immunofluorescence-img19530.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (AB269513)")
![Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (AB269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--immunocytochemistry-immunofluorescence-img20904.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (AB269513)")
Publications
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- Acta pharmaceutica Sinica. B 13:4875-48922023Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy regulation of TFRC-dependent ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Yu,Yuehuai Hu,Zhiping Liu,Kaiteng Guo,Dinghu Ma,Mingxia Peng,Yuemei Wang,Jing Zhang,Xiaolei Zhang,Panxia Wang,Jiguo Zhang,Peiqing Liu,Jing LuPubMed 38045054
- International journal of molecular medicine 52:2023Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayin Sun,Yihua Wang,Yilun Du,Wanxin Zhang,Zuodong Liu,Jin Bai,Guanqun Cui,Zhongjun DuPubMed 37264973
- Free radical biology & medicine 199:97-1122023Melatonin ameliorates neurological deficits through MT2/IL-33/ferritin H signaling-mediated inhibition of neuroinflammation and ferroptosis after traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Gao,Tao Wang,Ying Cheng,Yumin Wu,Luwen Zhu,Zhiya Gu,Youzhuang Wu,Luwei Cai,Yimin Wu,Yidan Zhang,Cheng Gao,Lili Li,Jing Li,Qianqian Li,Zufeng Wang,Ying Wang,Fudi Wang,Chengliang Luo,Luyang TaoPubMed 36805045
- Cell death & disease 13:10002022Identification of a group of bisbenzylisoquinoline (BBIQ) compounds as ferroptosis inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYipu Fan,Yihan Zhang,Kunyu Shi,Shan Cheng,Duanqing Pei,Xiaodong ShuPubMed 36435804
- Environmental toxicology 38:289-2992022Bone marrow mesenchymal stem cells repair hexavalent chromium-induced testicular injury by regulating autophagy and ferroptosis mediated by the AKT/mTOR pathway in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuijian Zhuge,Zhongrun Li,Changhao He,Wenxuan Ma,Jun Yan,Qian Xue,Rui Wang,Ying Liu,Rifeng Lu,Haiying Du,Fei Yin,Li GuoPubMed 36416502
- Pharmaceutical biology 60:1449-14572022A novobiocin derivative, XN4, triggers ferroptosis in gastric cancer cells via the activation of NOX4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongrong Li,Bin Yin,Deyu Zeng,Zhenyang LiuPubMed 35938505
- Oxidative medicine and cellular longevity 2022:89799042022-Lipoic Acid-Plus Ameliorates Endothelial Injury by Inhibiting the Apoptosis Pathway Mediated by Intralysosomal Cathepsins in an and Endothelial Injury Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Wang,Dejun Bao,Yongfei Dong,Xiangpin Wei,Jian Yu,Chaoshi NiuPubMed 35450412
- Frontiers in pharmacology 12:8084802022Fisetin Attenuates Doxorubicin-Induced Cardiomyopathy and by Inhibiting Ferroptosis Through SIRT1/Nrf2 Signaling Pathway Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanlei Li,Xiaoman Liu,Wenhu Pi,Yang Zhang,Lei Yu,Cheng Xu,Zhenzhu Sun,Jianjun JiangPubMed 35273493
- Frontiers in molecular biosciences 8:6600722021Cox4i2 Triggers an Increase in Reactive Oxygen Species, Leading to Ferroptosis and Apoptosis in HHV7 Infected Schwann Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBowen Chang,Haochen Guan,Xueyi Wang,Zheng Chen,Wanchun Zhu,Xiangyu Wei,Shiting LiPubMed 34026834
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Not recommended |
| Rat | Tested | Expected | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/5000 | Notes - |
Species Human | Dilution info 1/5000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/25 | Notes - |
Species Human | Dilution info 1/25 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738). Mediates uptake of NICOL1 into fibroblasts where it may regulate extracellular matrix production (By similarity). (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Alternative names
CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC
Recommended products
Mouse Recombinant Monoclonal Transferrin Receptor antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 9 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- H68.4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.
- Biological function summary
TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.
- Pathways
The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.
- Associated diseases and disorders
Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--western-blot-img82785.jpg "Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513)")
Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513)
Transferrin receptor can undergo glycosylation as shown in lane 4 and 5 (PMID: 30854239).
Blocking/Diliution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1/3-5: 114 seconds; Lane 2: 3 minutes.
All lanes: Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513) at 1/5000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg
Lane 3: Human placenta tissue lysate at 10 µg
Lane 4: Mouse placenta tissue lysate at 10 µg
Lane 5: Rat placenta tissue lysate at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 84 kDa
Observed band size: 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113519.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on human placenta. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17671.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513)
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on human cervical carcinoma. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--immunocytochemistry-immunofluorescence-img19530.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513)")
Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K-562 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--immunocytochemistry-immunofluorescence-img20904.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513)")
Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--western-blot-img436860.jpg "Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513)")
Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513)
Blocking and diluting beffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control at 1/200000 dilution.
The blot of ab269513 is applied with Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) secondary antibody at 1/10000 dilution and the blot of Anti-Transferrin Receptor antibody [EPR20584] ab214039 is applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100000 dilution.
Lanes 1 - 9: Western blot - Anti-Transferrin Receptor antibody [EPR20584] (Anti-Transferrin Receptor antibody [EPR20584] ab214039) at 1/1000 dilution
Lanes 1 - 9: Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 20 µg
Lane 2: Human small intestine tissue lysate at 20 µg
Lane 3: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: LoVo (human colorectal adenocarcinoma epithelial cell)whole cell lysate at 20 µg
Lane 5: Mouse placenta tissue lysate at 20 µg
Lane 6: Mouse kidney tissue lysate at 20 µg
Lane 7: Mouse heart tissue lysate at 20 µg
Lane 8: Rat placenta tissue lysate at 20 µg
Lane 9: Rat kidney tissue lysate at 20 µg
Predicted band size: 84 kDa
Observed band size: 90 kDa
Exposure time: 26s
![Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513), expandable thumbnail](https://content.abcam.com/products/images/transferrin-receptor-antibody-h684-ab269513--western-blot-img436860.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513)")
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