Anti-Transferrin Receptor antibody [H68.4]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (AB269513)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human placenta. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (AB269513)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K-562 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (AB269513)

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cervical carcinoma. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (AB269513)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

• WB

Lab

Western blot - Anti-Transferrin Receptor antibody [H68.4] (AB269513)

Transferrin receptor can undergo glycosylation as shown in lane 4 and 5 (PMID : 30854239).

Blocking/Diliution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1/3-5 : 114 seconds; Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513) at 1/5000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 3:

Human placenta tissue lysate at 10 µg

Lane 4:

Mouse placenta tissue lysate at 10 µg

Lane 5:

Rat placenta tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 84 kDa

Observed band size: 90 kDa

false

• WB

Lab

Western blot - Anti-Transferrin Receptor antibody [H68.4] (AB269513)

Blocking and diluting beffer and concentration : 5% NFDM/TBST.

ab181602 was used as a GAPDH loading control at 1/200000 dilution.

The blot of ab269513 is applied with Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) secondary antibody at 1/10000 dilution and the blot of ab214039 is applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100000 dilution.

Lanes 1 - 9:

Western blot - Anti-Transferrin Receptor antibody [EPR20584] (<a href='/en-us/products/primary-antibodies/transferrin-receptor-antibody-epr20584-ab214039'>ab214039</a>) at 1/1000 dilution

Lanes 1 - 9:

Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human small intestine tissue lysate at 20 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

LoVo (human colorectal adenocarcinoma epithelial cell)whole cell lysate at 20 µg

Lane 5:

Mouse placenta tissue lysate at 20 µg

Lane 6:

Mouse kidney tissue lysate at 20 µg

Lane 7:

Mouse heart tissue lysate at 20 µg

Lane 8:

Rat placenta tissue lysate at 20 µg

Lane 9:

Rat kidney tissue lysate at 20 µg

Predicted band size: 84 kDa

Observed band size: 90 kDa

false

Exposure time: 26s