AB1086

Anti-Transferrin Receptor antibody [MEM-189]

Name: Anti-Transferrin Receptor antibody [MEM-189] (ab1086) | Abcam
Brand: Abcam Limited
SKU: AB1086
Rating: 5 (4 reviews)

(4 Reviews)

(38 Publications)

Anti-Transferrin Receptor antibody [MEM-189] (ab1086) is a mouse monoclonal antibody detecting Transferrin Receptor in Flow Cytometry. Suitable for Human.

- Over 30 publications
- Trusted since 2002

View Alternative Names

CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC

1 Images

Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-189] (AB1086)

Flow Cyt

Unknown

Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-189] (AB1086)

Flow Cytometry analysis of K562 cells labelling Transferrin with ab1086 at 2 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-189

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing TFRC protein. The exact immunogen used to generate this antibody is proprietary information.

P02786

Specificity

The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes.The antibody MEM-189 does not block binding of transferrin to the receptor.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Transferrin Receptor antibody [MEM-189] (ab1086) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt) in Human samples.

Trusted by the scientific community
Anti-Transferrin Receptor [MEM-189] (ab1086) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [MEM-189] also available for your convenience: ab1086, Carrier free - ab313322

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification technique

Affinity purification Protein A

Storage buffer

pH: 8 Preservative: 0.097% Sodium azide Constituents: Tris buffer

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.

Biological function summary

TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.

Pathways

The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.

Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene TFRC is involved in the cellular uptake of iron via receptor-mediated endocytosis, where the transferrin receptor, bound to its ligand, is internalized into specialized endosomes. Following endosomal acidification, iron is released, and the apotransferrin-receptor complex is recycled to the cell surface, where a return to neutral pH results in the loss of apotransferrin's affinity for its receptor. The hereditary hemochromatosis protein HFE competes with transferrin for binding at an overlapping C-terminal site. TFRC positively regulates T and B cell proliferation through iron uptake. It functions as a lipid sensor influencing mitochondrial fusion by modulating the JNK pathway activity. Low dietary levels of stearate promote JNK pathway activation, leading to HUWE1-mediated ubiquitination and degradation of the mitofusin MFN2, inhibiting mitochondrial fusion. High dietary stearate levels result in TFRC stearoylation, which inhibits JNK pathway activation and MFN2 degradation. Additionally, TFRC acts as a receptor for new-world arenaviruses, including Guanarito, Junin, and Machupo viruses. This supplementary information is collated from multiple sources and compiled automatically.

See full target information TFRC

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Diabetes, metabolic syndrome and obesity : targets and therapy 17:435-452 PubMed38299195

2024

Electroacupuncture Reduces Inflammatory Bowel Disease in Obese Mice by Activating the Nrf2/HO-1 Signaling Pathways and Repairing the Intestinal Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhao Yang,Fang Pang,Min Zhou,Xiao Guo,Yan Yang,Wei Qiu,Cai Liao,Yang Chen,Chenglin Tang

Aging 16:2181-2193 PubMed38277217

2024

(-)-Epicatechin protects against myocardial ischemia/reperfusion injury via autophagy-dependent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kong Junhong,Tsai Yun,Shui Guangxing,Ding Yuhan,Xiang Qian,Zhang Haowen

Cellular and molecular life sciences : CMLS 80:135 PubMed37119365

2023

A structural model of the iRhom-ADAM17 sheddase complex reveals functional insights into its trafficking and activity.

Applications

Unspecified application

Species

Unspecified reactive species

Selcan Kahveci-Türköz,Katharina Bläsius,Justyna Wozniak,Cindy Rinkens,Anke Seifert,Petr Kasparek,Henrike Ohm,Shixin Oltzen,Martin Nieszporek,Nicole Schwarz,Aaron Babendreyer,Christian Preisinger,Radislav Sedlacek,Andreas Ludwig,Stefan Düsterhöft

Frontiers in immunology 13:985538 PubMed36713405

2023

Low circulatory Fe and Se levels with a higher IL-6/IL-10 ratio provide nutritional immunity in tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sandeep R Kaushik,Sukanya Sahu,Hritusree Guha,Sourav Saha,Ranjit Das,Rukuwe-U Kupa,Wetetsho Kapfo,Trinayan Deka,Rumi Basumatary,Asunu Thong,Arunabha Dasgupta,Bidhan Goswami,Amit Kumar Pandey,Lahari Saikia,Vinotsole Khamo,Anjan Das,Ranjan Kumar Nanda

Diabetology & metabolic syndrome 14:89 PubMed35761309

2022

Targeting ferroptosis with miR-144-3p to attenuate pancreatic β cells dysfunction via regulating USP22/SIRT1 in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Zhang,Xiao Liu,Jihong Wang,Fengjuan Yuan,Yali Liu

Bio-protocol 12:e4399 PubMed35800091

2022

High Throughput Blood-brain Barrier Organoid Generation and Assessment of Receptor-Mediated Antibody Transcytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Kassianidou,Claire Simonneau,Alina Gavrilov,Roberto Villaseñor

Acta pharmacologica Sinica 42:1690-1702 PubMed34112960

2021

ATF3 contributes to brucine-triggered glioma cell ferroptosis via promotion of hydrogen peroxide and iron.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Lu,Xuan-Zhong Wang,Chuan He,Lei Wang,Shi-Peng Liang,Chong-Cheng Wang,Chen Li,Tian-Fei Luo,Chun-Sheng Feng,Zhen-Chuan Wang,Guang-Fan Chi,Peng-Fei Ge

Evidence-based complementary and alternative medicine : eCAM 2021:6864723 PubMed34135983

2021

(SM) Injection Ameliorates Iron Overload-Associated Cardiac Dysfunction by Regulating the Expression of DMT1, TfR1, and FP1 in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Zhang,Yucong Xue,Bin Zheng,Xue Han,Donglai Ma,Zhihong Ma,Shengjiang Guan,Yonggang Gao,Ziliang Li,Li Chu

Cellular and molecular life sciences : CMLS 78:5015-5040 PubMed33950315

2021

The iRhom homology domain is indispensable for ADAM17-mediated TNFα and EGF receptor ligand release.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Düsterhöft,Selcan Kahveci-Türköz,Justyna Wozniak,Anke Seifert,Petr Kasparek,Henrike Ohm,Shixin Liu,Jana Kopkanova,Juliane Lokau,Christoph Garbers,Christian Preisinger,Radislav Sedlacek,Matthew Freeman,Andreas Ludwig

Neurochemical research 46:1239-1251 PubMed33646533

2021

Benefits of Iron Chelators in the Treatment of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Zeng,Hedi An,Fei Yu,Kai Wang,Lanlan Zheng,Wei Zhou,Yiwen Bao,Jie Yang,Nan Shen,Dongya Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Transferrin Receptor antibody [MEM-189]

Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-189] (AB1086)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

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