Rabbit Recombinant Monoclonal Transglutaminase 2 antibody. Carrier free. Suitable for IP, ICC/IF, IHC-P, WB and reacts with Mouse samples.
Images
![Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--western-blot-img445559.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809)")
![Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--western-blot-img445560.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809)")
![Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--western-blot-img445561.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809)")
![Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunocytochemistry-immunofluorescence-img445604.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445601.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (AB323809)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively (By similarity). Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis (By similarity). Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54 (PubMed:11113189, PubMed:11274171, PubMed:20489165). Under physiological conditions, the protein cross-linking activity is inhibited by GTP; inhibition is relieved by Ca(2+) in response to various stresses (By similarity). When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds (By similarity). Plays a key role during apoptosis, both by (1) promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm, and by (2) mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components (PubMed:12810961, PubMed:15905580). In addition to protein cross-linking, can use different monoamine substrates to catalyze a vast array of protein post-translational modifications: mediates aminylation of serotonin, dopamine, noradrenaline or histamine into glutamine residues of target proteins to generate protein serotonylation, dopaminylation, noradrenalinylation or histaminylation, respectively (PubMed:32116663). Mediates protein serotonylation of small GTPases during activation and aggregation of platelets, leading to constitutive activation of these GTPases (By similarity). Plays a key role in chromatin organization by mediating serotonylation and dopaminylation of histone H3 (By similarity). Catalyzes serotonylation of 'Gln-5' of histone H3 (H3Q5ser) during serotonergic neuron differentiation, thereby facilitating transcription (By similarity). Acts as a mediator of neurotransmission-independent role of nuclear dopamine in ventral tegmental area (VTA) neurons: catalyzes dopaminylation of 'Gln-5' of histone H3 (H3Q5dop), thereby regulating relapse-related transcriptional plasticity in the reward system (By similarity). Regulates vein remodeling by mediating serotonylation and subsequent inactivation of ATP2A2/SERCA2 (PubMed:32116663). Also acts as a protein deamidase by mediating the side chain deamidation of specific glutamine residues of proteins to glutamate (By similarity). Catalyzes specific deamidation of protein gliadin, a component of wheat gluten in the diet (By similarity). May also act as an isopeptidase cleaving the previously formed cross-links (By similarity). Also able to participate in signaling pathways independently of its acyltransferase activity: acts as a signal transducer in alpha-1 adrenergic receptor-mediated stimulation of phospholipase C-delta (PLCD) activity and is required for coupling alpha-1 adrenergic agonists to the stimulation of phosphoinositide lipid metabolism (PubMed:11274171).
Alternative names
Protein-glutamine gamma-glutamyltransferase 2, Isopeptidase TGM2, Protein-glutamine deamidase TGM2, Protein-glutamine dopaminyltransferase TGM2, Protein-glutamine histaminyltransferase TGM2, Protein-glutamine noradrenalinyltransferase TGM2, Protein-glutamine serotonyltransferase TGM2, Tissue transglutaminase, Transglutaminase II, Transglutaminase-2, tTG, TGase II, TG2, TGase-2, TGase2, Tgm2
Rabbit Recombinant Monoclonal Transglutaminase 2 antibody. Carrier free. Suitable for IP, ICC/IF, IHC-P, WB and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29853-527
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab323809 is the carrier free version of Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--western-blot-img445559.jpg "Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)
Transglutaminase 2 Western blot staining using rabbit Anti-Transglutaminase 2 antibody
This data was developed using Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] (Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793) at 1/1000 dilution
Lane 1: Hepa1-6 (mouse hepatoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: Hepa1-6 transfected with siRNA specifically targeting TGM2 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa, 36 kDa
Exposure time: 15s
![Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--western-blot-img445560.jpg "Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)
Transglutaminase 2 Western blot staining using rabbit Anti-Transglutaminase 2 antibody
This data was developed using Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: EL4, A20.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] (Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793) at 1/1000 dilution
Lane 1: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg
Lane 5: A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa, 36 kDa
Exposure time: 26s
![Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--western-blot-img445561.jpg "Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)
Transglutaminase 2 Western blot staining using rabbit Anti-Transglutaminase 2 antibody
This data was developed using Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Transglutaminase 2 antibody [EPR29853-527] (Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse testis tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa, 36 kDa
Exposure time: 26s
![Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunocytochemistry-immunofluorescence-img445604.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)
Transglutaminase 2 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Transglutaminase 2 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling Transglutaminase 2 with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 at 1/50 (10.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in Hepa1-6 and no staining in EL4 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression: EL4.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445601.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)
Transglutaminase 2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-Transglutaminase 2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Transglutaminase 2 with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: positive staining on blood vessels of mouse cerebrum. The section was incubated with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445600.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)
Transglutaminase 2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-Transglutaminase 2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Transglutaminase 2 with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse liver. The section was incubated with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445599.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)
Transglutaminase 2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse pancreas carcinoma using rabbit Anti-Transglutaminase 2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse pancreas carcinoma tissue labeling Transglutaminase 2 with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse pancreas carcinoma. The section was incubated with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445598.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)
Transglutaminase 2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spleen using rabbit Anti-Transglutaminase 2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Transglutaminase 2 with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 at 1/500 (1.006 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen. The section was incubated with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunoprecipitation - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunoprecipitation-img445531.jpg "Immunoprecipitation - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
Immunoprecipitation - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)
This data was developed using Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793, the same antibody clone in a different buffer formulation.
Transglutaminase 2 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate with Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-Transglutaminase 2 antibody [EPR29853-527] (Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793) at 1/1000 dilution
Lane 1: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 10 µg
Lane 2: Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 at 1/30 IP in Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 10 µg
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Transglutaminase 2 antibody [EPR29853-527] ab323793 in Hepa1-6 whole cell lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366)
Observed band size: 75 kDa
Exposure time: 67s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445600.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445599.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445598.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
![Immunoprecipitation - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809), expandable thumbnail](https://content.abcam.com/products/images/transglutaminase-2-antibody-epr29853-527-bsa-and-azide-free-ab323809--immunoprecipitation-img445531.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Transglutaminase 2 antibody [EPR29853-527] - BSA and Azide free (ab323809)")
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