Rabbit Polyclonal Transglutaminase 5/TGM5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TGM5.
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- Biomedicines 11:2023New Data on the Features of Skin Barrier in Hidradenitis Suppurativa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOrsolya Somogyi,Zsolt Dajnoki,Lilla Szabó,Krisztián Gáspár,Zoltán Hendrik,Christos C Zouboulis,Klaudia Dócs,Péter Szücs,Katalin Dull,Dániel Törőcsik,Anikó Kapitány,Andrea SzegediPubMed 36672635
- International journal of molecular sciences 22:2021Regional Differences in the Permeability Barrier of the Skin-Implications in Acantholytic Skin Diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnikó Kapitány,Barbara Medgyesi,Adrienn Jenei,Orsolya Somogyi,Lilla Szabó,Krisztián Gáspár,Gábor Méhes,Zoltán Hendrik,Klaudia Dócs,Péter Szücs,Zsolt Dajnoki,Andrea SzegediPubMed 34638769
- The Journal of investigative dermatology 140:1938-1950.e52020Rosacea Is Characterized by a Profoundly Diminished Skin Barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Medgyesi et. al.PubMed 32199994
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TGM5. The exact immunogen used to generate this antibody is proprietary information. Database link O43548
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Contributes to the formation of the cornified cell envelope of keratinocytes.
Alternative names
TGMX, TGM5, Protein-glutamine gamma-glutamyltransferase 5, Transglutaminase X, Transglutaminase-5, TG(X), TGX, TGase X, TGase-5
Recommended products
Rabbit Polyclonal Transglutaminase 5/TGM5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TGM5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TGM5. The exact immunogen used to generate this antibody is proprietary information. Database link O43548
- Purification technique
- Affinity purification Immunogen
- Specificity
ab133786 is predicted to not cross-react with other Transglutaminase protein family members. Multiple isoforms of Transglutaminase 5 are known to exist.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transglutaminase 5 (TGM5) also known as TGase 5 or TG5 is an enzyme part of the transglutaminase family. This enzyme has a molecular mass of about 78 kDa and plays a role in catalyzing the formation of epsilon-(gamma-glutamyl) lysine isopeptide bonds. These covalent bonds contribute to the stability and mechanical properties of proteins. TGM5 is expressed in various tissues with a strong presence in the epidermis indicating its significance in skin cell function and differentiation.
- Biological function summary
TGM5 participates in skin barrier formation and homeostasis. TGM5 acts as a contributor to the crosslinking of structural proteins in the stratum corneum the outermost skin layer. It functions collaboratively integrating with other enzymes within a network that ensures the integrity of the epidermal barrier. This process is essential for skin hydration and resistance against environmental stressors.
- Pathways
TGM5 is involved in the keratinocyte differentiation pathway and epidermal barrier repair processes. It works closely alongside proteins such as involucrin and loricrin playing a role in the cornified envelope formation. These proteins and pathways are important for the maintenance of the skin's protective functions and for providing resilience against damage.
- Associated diseases and disorders
TGM5 is primarily associated with skin-related conditions such as peeling skin syndrome (PSS) and lamellar ichthyosis. Mutations in the TGM5 gene can disrupt its enzymatic function leading to compromised epidermal barrier integrity. In PSS TGM5 dysfunction can result in abnormal exfoliation of the skin while links between TGM5 and loricrin underline the complexity of keratinization disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 5/TGM5 antibody (ab133786)
Immunohistochemical analysis of formalin fixed, paraffin-embedded Human spleen tissue labelling Transglutaminase 5/TGM5 with ab133786 at 5 μg/ml. Primary antibody labelling was followed by a biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 5/TGM5 antibody (ab133786)
Immunohistochemical analysis of formalin fixed, paraffin-embedded Human liver tissue labelling Transglutaminase 5/TGM5 with ab133786 at 5 μg/ml. Primary antibody labelling was followed by a biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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