AB133786

Anti-Transglutaminase 5/TGM5 antibody

Name: Anti-Transglutaminase 5/TGM5 antibody (ab133786) | Abcam
Brand: Abcam Limited
SKU: AB133786
Price: 565 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Transglutaminase 5/TGM5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TGM5.

View Alternative Names

TGMX, TGM5, Protein-glutamine gamma-glutamyltransferase 5, Transglutaminase X, Transglutaminase-5, TG(X), TGX, TGase X, TGase-5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 5/TGM5 antibody (AB133786)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 5/TGM5 antibody (AB133786)

Immunohistochemical analysis of formalin fixed, paraffin-embedded Human spleen tissue labelling Transglutaminase 5/TGM5 with ab133786 at 5 μg/ml. Primary antibody labelling was followed by a biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 5/TGM5 antibody (AB133786)

Immunohistochemical analysis of formalin fixed, paraffin-embedded Human liver tissue labelling Transglutaminase 5/TGM5 with ab133786 at 5 μg/ml. Primary antibody labelling was followed by a biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human TGM5. The exact immunogen used to generate this antibody is proprietary information.

O43548

Specificity

ab133786 is predicted to not cross-react with other Transglutaminase protein family members. Multiple isoforms of Transglutaminase 5 are known to exist.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transglutaminase 5 (TGM5) also known as TGase 5 or TG5 is an enzyme part of the transglutaminase family. This enzyme has a molecular mass of about 78 kDa and plays a role in catalyzing the formation of epsilon-(gamma-glutamyl) lysine isopeptide bonds. These covalent bonds contribute to the stability and mechanical properties of proteins. TGM5 is expressed in various tissues with a strong presence in the epidermis indicating its significance in skin cell function and differentiation.

Biological function summary

TGM5 participates in skin barrier formation and homeostasis. TGM5 acts as a contributor to the crosslinking of structural proteins in the stratum corneum the outermost skin layer. It functions collaboratively integrating with other enzymes within a network that ensures the integrity of the epidermal barrier. This process is essential for skin hydration and resistance against environmental stressors.

Pathways

TGM5 is involved in the keratinocyte differentiation pathway and epidermal barrier repair processes. It works closely alongside proteins such as involucrin and loricrin playing a role in the cornified envelope formation. These proteins and pathways are important for the maintenance of the skin's protective functions and for providing resilience against damage.

TGM5 is primarily associated with skin-related conditions such as peeling skin syndrome (PSS) and lamellar ichthyosis. Mutations in the TGM5 gene can disrupt its enzymatic function leading to compromised epidermal barrier integrity. In PSS TGM5 dysfunction can result in abnormal exfoliation of the skin while links between TGM5 and loricrin underline the complexity of keratinization disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Contributes to the formation of the cornified cell envelope of keratinocytes.

See full target information TGM5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:1932-1946 PubMed40646310

2025

Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Geraldine Rodschinka,Sergio Forcelloni,Felix M Kühner,Sascha Wani,Henrick Riemenschneider,Dieter Edbauer,Andrew Behrens,Danny D Nedialkova

Biomedicines 11: PubMed36672635

2023

New Data on the Features of Skin Barrier in Hidradenitis Suppurativa.

Applications

Unspecified application

Species

Unspecified reactive species

Orsolya Somogyi,Zsolt Dajnoki,Lilla Szabó,Krisztián Gáspár,Zoltán Hendrik,Christos C Zouboulis,Klaudia Dócs,Péter Szücs,Katalin Dull,Dániel Törőcsik,Anikó Kapitány,Andrea Szegedi

International journal of molecular sciences 22: PubMed34638769

2021

Regional Differences in the Permeability Barrier of the Skin-Implications in Acantholytic Skin Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Anikó Kapitány,Barbara Medgyesi,Adrienn Jenei,Orsolya Somogyi,Lilla Szabó,Krisztián Gáspár,Gábor Méhes,Zoltán Hendrik,Klaudia Dócs,Péter Szücs,Zsolt Dajnoki,Andrea Szegedi

The Journal of investigative dermatology 140:1938-1950.e5 PubMed32199994

2020

Rosacea Is Characterized by a Profoundly Diminished Skin Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Medgyesi,Zsolt Dajnoki,Gabriella Béke,Krisztián Gáspár,Imre Lőrinc Szabó,Eszter Anna Janka,Szilárd Póliska,Zoltán Hendrik,Gábor Méhes,Dániel Törőcsik,Tamás Bíró,Anikó Kapitány,Andrea Szegedi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Transglutaminase 5/TGM5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 5/TGM5 antibody (AB133786)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 5/TGM5 antibody (AB133786)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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