Anti-Transketolase antibody [7H1AA1]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody [7H1AA1] (AB112997)

IHC image of Transketolase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112997, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Transketolase antibody [7H1AA1] (AB112997)

ab112997 at 1ug/ml staining Transketolase in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI (blue). Non specific fluorescence was reduced using a signal enhancer.

• Flow Cyt

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Flow Cytometry - Anti-Transketolase antibody [7H1AA1] (AB112997)

ab112997 at 1ug/ml staining Transketolase in HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).

• WB

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Western blot - Anti-Transketolase antibody [7H1AA1] (AB112997)

All lanes:

Western blot - Anti-Transketolase antibody [7H1AA1] (ab112997) at 1 µg/mL

Lane 1:

HepG2 Cell Lysate at 20 µg

Lane 2:

HeLa Cell Lysate at 20 µg

Predicted band size: 68 kDa

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