Rabbit Polyclonal Transketolase antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Transketolase aa 1-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Transketolase aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P29401
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.20000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Chimpanzee, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Chimpanzee, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.
Alternative names
Transketolase, TK, TKT
Recommended products
Rabbit Polyclonal Transketolase antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Transketolase aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Transketolase aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P29401
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
ab233179 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transketolase (TKT) is an important enzyme that plays an important mechanical role in the pentose phosphate pathway by catalyzing the transfer of ketol groups from ketose donors to aldose acceptors. This process forms part of the non-oxidative segment of the pathway converting ribose 5-phosphate into other sugars equipped for nucleotide synthesis and energy metabolism. TKT is also recognized under the name erythrocyte transketolase as it is found in high abundance in erythrocytes. The enzyme has a molecular mass of around 73 kDa and expresses broadly in human tissues especially within liver and fat cells where it supports carbohydrate metabolism.
- Biological function summary
Transketolase participates in critical cellular metabolic processes facilitating cellular growth and repair by supporting the synthesis of nucleotides and amino acids. It does not function alone but rather operates as part of a complex with other enzymes such as transaldolase within the pentose phosphate pathway. The direct activity of erythrocyte transketolase can be essential for maintaining redox balance and providing intermediates that impact cell proliferation and antioxidant defense mechanisms.
- Pathways
With regard to pathways involved in carbohydrate metabolism TKT plays an important role in the pentose phosphate pathway and glycolysis. In the pentose phosphate pathway TKT collaborates with enzymes like glucose-6-phosphate dehydrogenase to provide ribose-5-phosphate for nucleic acid synthesis and NADPH for reductive biosynthesis and oxidative stress defense. Its relationship with transaldolase in this pathway showcases a tightly regulated enzymatic network that maintains cellular homeostasis.
- Associated diseases and disorders
TKT is associated with metabolic dysfunctions such as diabetes and certain neurodegenerative diseases including Wernicke's encephalopathy. In diabetes changes in transketolase activity can disturb glucose metabolism and contribute to oxidative stress linking it subconsciously with enzymes like phospholipase C which play roles in lipid signaling and cellular responses. Additionally reduced erythrocyte transketolase activity connects with thiamine deficiency in Wernicke's encephalopathy further affecting brain functions and metabolic pathways relying on proper transketolase function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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9 product images
Western blot - Anti-Transketolase antibody (ab233179)
All lanes: Western blot - Anti-Transketolase antibody (ab233179) at 1 µg/mL
All lanes: A549 (human lung carcinoma cell line) cell lysate
SecondaryAll lanes: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL
Predicted band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody (ab233179)
Formalin-fixed, paraffin-embedded human thyroid tissue stained for Transketolase using ab233179 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody (ab233179)
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Transketolase using ab233179 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody (ab233179)
Formalin-fixed, paraffin-embedded human glioma tissue stained for Transketolase using ab233179 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody (ab233179)
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Transketolase using ab233179 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody (ab233179)
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Transketolase using ab233179 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody (ab233179)
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Transketolase using ab233179 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody (ab233179)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Transketolase using ab233179 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Western blot - Anti-Transketolase antibody (ab233179)
All lanes: Western blot - Anti-Transketolase antibody (ab233179) at 2 µg/mL
All lanes: Recombinant human Transketolase protein
Predicted band size: 68 kDa
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