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Rabbit Recombinant Monoclonal Transmembrane 4 L6 family member 1 antibody. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342), expandable thumbnail
  • Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Target data

Function

Alternative names

Transmembrane 4 L6 family member 1, Membrane component chromosome 3 surface marker 1, Tumor-associated antigen L6, M3S1, TAAL6, TM4SF1

Rabbit Recombinant Monoclonal Transmembrane 4 L6 family member 1 antibody. Suitable for IHC-P, WB and reacts with Human samples.

Alternative names

Transmembrane 4 L6 family member 1, Membrane component chromosome 3 surface marker 1, Tumor-associated antigen L6, M3S1, TAAL6, TM4SF1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28894-539

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342), expandable thumbnail

    Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Performed under reducing conditions.

    In Western blot, ab322342 was shown to bind specifically to Transmembrane 4 L6 family member 1. Target of interest was observed at 19 kDa and 26 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in TM4SF1 knockout cell line (lane 2) (lane 2, knockout cell line Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell line ab265743 / knockout cell lysate Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell lysate ab258232).

    Negative control: Jurkat (PMID: 1565644).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 2: TM4SF1 knockout HeLa whole cell lysate at 50 µg

    Lane 3: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 26 kDa, 19 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342), expandable thumbnail

    Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Transmembrane 4 L6 family member 1 is a glycoprotein of approximately 26 kDa and detected as a 19-kDa band after treated with Protein Deglycosylation MIX II.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342) at 1/1000 dilution

    Lane 1: Untreated A549 (human lung carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 2: A549 whole cell lysate treated with Protein Deglycosylation MIX II at 50 µg

    Lane 3: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 4: HeLa whole cell lysate treated with Protein Deglycosylation MIX II at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 26 kDa, 19 kDa, 36 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human colon.

    The section was incubated with ab322342 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342)

    Immunohistochemical analysis of paraffin-embedded (A) Human stomach adenocarcinoma and (B) adjacent stomach tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in (A) human stomach adenocarcinoma, positive staining in endothelia of (B) adjacent human stomach.

    The section was incubated with ab322342 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342)

    Immunohistochemical analysis of paraffin-embedded (A) Wild-type Hela (human cervical adenocarcinoma epithelial cell) cell pellet. (B) TM4SF1 knockout HeLa cell pellet. tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in (A) Wild-type Hela cell pellet, no staining in (B) TM4SF1 knockout HeLa cell pellet.

    The section was incubated with ab322342 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human breast carcinoma.

    The section was incubated with ab322342 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in endothelia of human cardiac muscle.

    The section was incubated with ab322342 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human prostatic hyperplasia.

    The section was incubated with ab322342 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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