Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human colon.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342)

Immunohistochemical analysis of paraffin-embedded (A) Human stomach adenocarcinoma and (B) adjacent stomach tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) human stomach adenocarcinoma, positive staining in endothelia of (B) adjacent human stomach.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type Hela (human cervical adenocarcinoma epithelial cell) cell pellet and (B) TM4SF1 knockout HeLa cell pellet labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) Wild-type Hela cell pellet, no staining in (B) TM4SF1 knockout HeLa cell pellet.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human breast carcinoma.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in endothelia of human cardiac muscle.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human prostatic hyperplasia.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab322342 was shown to bind specifically to Transmembrane 4 L6 family member 1. Target of interest was observed at 19 kDa and 26 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in TM4SF1 knockout cell line (lane 2) (lane 2, knockout cell line ab265743 / knockout cell lysate ab258232).

Negative control : Jurkat (PMID : 1565644).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

TM4SF1 knockout HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,19 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (AB322342)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Transmembrane 4 L6 family member 1 is a glycoprotein of approximately 26 kDa and detected as a 19-kDa band after treated with Protein Deglycosylation MIX II.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (ab322342) at 1/1000 dilution

Lane 1:

Untreated A549 (human lung carcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

A549 whole cell lysate treated with Protein Deglycosylation MIX II at 50 µg

Lane 3:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate treated with Protein Deglycosylation MIX II at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,19 kDa,36 kDa

false

Exposure time: 180s