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AB324786

Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free

  • KO Validated
  • RabMAb
  • Recombinant
  • BOND RX™ Validated
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Rabbit Recombinant Monoclonal Transmembrane 4 L6 family member 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

M3S1, TAAL6, TM4SF1, Transmembrane 4 L6 family member 1, Membrane component chromosome 3 surface marker 1, Tumor-associated antigen L6

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)

This data was developed using ab322342, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human colon.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)

This data was developed using ab322342, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Human stomach adenocarcinoma and (B) adjacent stomach tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) human stomach adenocarcinoma, positive staining in endothelia of (B) adjacent human stomach.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)

This data was developed using ab322342, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Wild-type Hela (human cervical adenocarcinoma epithelial cell) cell pellet and (B) TM4SF1 knockout HeLa cell pellet labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) Wild-type Hela cell pellet, no staining in (B) TM4SF1 knockout HeLa cell pellet.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)

This data was developed using ab322342, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human breast carcinoma.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)

This data was developed using ab322342, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in endothelia of human cardiac muscle.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)

This data was developed using ab322342, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Transmembrane 4 L6 family member 1 with ab322342 at 1/1000 (0.518 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human prostatic hyperplasia.
The section was incubated with ab322342 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)
  • WB

Supplier Data

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)

This data was developed using ab322342, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab322342 was shown to bind specifically to Transmembrane 4 L6 family member 1. Target of interest was observed at 19 kDa and 26 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in TM4SF1 knockout cell line (lane 2) (lane 2, knockout cell line ab265743 / knockout cell lysate ab258232).

Negative control : Jurkat (PMID : 1565644).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (<a href='/en-us/products/primary-antibodies/transmembrane-4-l6-family-member-1-antibody-epr28894-539-ab322342'>ab322342</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

TM4SF1 knockout HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,19 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)
  • WB

Supplier Data

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] - BSA and Azide free (AB324786)

This data was developed using ab322342, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Transmembrane 4 L6 family member 1 is a glycoprotein of approximately 26 kDa and detected as a 19-kDa band after treated with Protein Deglycosylation MIX II.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539] (<a href='/en-us/products/primary-antibodies/transmembrane-4-l6-family-member-1-antibody-epr28894-539-ab322342'>ab322342</a>) at 1/1000 dilution

Lane 1:

Untreated A549 (human lung carcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

A549 whole cell lysate treated with Protein Deglycosylation MIX II at 50 µg

Lane 3:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate treated with Protein Deglycosylation MIX II at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,19 kDa,36 kDa

false

Exposure time: 180s

  • Unconjugated

    Anti-Transmembrane 4 L6 family member 1 antibody [EPR28894-539]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28894-539

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab324786 is the carrier-free version of ab322342.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TM4SF1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com