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Rabbit Recombinant Monoclonal Transportin 1/MIP antibody. Suitable for Dot, IP, ICC/IF, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Transportin 1/MIP antibody [EPR29606-11] (AB324273), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Transportin 1/MIP antibody [EPR29606-11] (AB324273), expandable thumbnail
  • Dot Blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (AB324273), expandable thumbnail
  • Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (AB324273), expandable thumbnail
  • Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (AB324273), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIPICC/IFWBIHC-P
Human
Expected
Tested
Tested
Tested
Not recommended
Mouse
Expected
Tested
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:24753571). May mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed:11682607). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones (By similarity). In vitro, mediates nuclear import of SRP19 (PubMed:11682607). Mediates nuclear import of ADAR/ADAR1 isoform 1 and isoform 5 in a RanGTP-dependent manner (PubMed:19124606, PubMed:24753571). Main mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with the karyopherins KPNA1 and KPNA2 (PubMed:35446349). (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.

Alternative names

KPNB2, MIP1, TRN, TNPO1, Transportin-1, Importin beta-2, Karyopherin beta-2, M9 region interaction protein, MIP

Rabbit Recombinant Monoclonal Transportin 1/MIP antibody. Suitable for Dot, IP, ICC/IF, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29606-11
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273)

    Transportin 1/MIP Immunocytochemistry/ Immunofluorescence staining of NIH/3T3 (mouse embryonic fibroblast) using rabbit Anti-Transportin 1/MIP antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Transportin 1/MIP with ab324273 at 1/50 (10.33 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

    Confocal image showing nuclear and cytoplasmic staining in NIH/3T3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab324273 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273)

    Transportin 1/MIP Immunocytochemistry/ Immunofluorescence staining of HeLa (human cervical adenocarcinoma epithelial cell) using rabbit Anti-Transportin 1/MIP antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Transportin 1/MIP with ab324273 at 1/50 (10.33 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

    Confocal image showing nuclear and cytoplasmic staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab324273 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Dot Blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273), expandable thumbnail

    Dot Blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273)

    Transportin 1/MIP Dot Blot staining using rabbit Anti-Transportin 1/MIP antibody

    Dot blot analysis of Transportin 1/MIP using ab324273 at 1:1000 (0.517 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: His-tagged humanTNPO2 recombinant protein

    Lane2: His-tagged humanTNPO1 recombinant protein

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human TNPO2.

    In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273) at 1/1000 dilution

    Lane 1: His-tagged humanTNPO2 recombinant protein

    Lane 2: His-tagged humanTNPO1 recombinant protein

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273), expandable thumbnail

    Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273)

    Transportin 1/MIP Western blot staining using rabbit Anti-Transportin 1/MIP antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 103 seconds, Lanes 3-4: 37 seconds

    All lanes: Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 50 µg

    Lane 2: Mouse cerebellum tissue lysate at 50 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 50 µg

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 102 kDa

  • Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273), expandable thumbnail

    Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273)

    Transportin 1/MIP Western blot staining using rabbit Anti-Transportin 1/MIP antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

    Lane 2: HeLa transfected with siRNA specifically targeting Transportin 1/MIP whole cell lysate at 50 µg

    Lane 3: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 102 kDa, 36 kDa

    Exposure time: 59s

  • Immunoprecipitation - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273), expandable thumbnail

    Immunoprecipitation - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273)

    Transportin 1/MIP was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab324273 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324273 at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

    Lane 2: ab324273 at 1/30 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab324273 in NIH/3T3 whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 102 kDa

    Exposure time: 3s

  • Immunoprecipitation - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273), expandable thumbnail

    Immunoprecipitation - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273)

    Transportin 1/MIP was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab324273 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324273 at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

    Lane 2: ab324273 at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab324273 in HeLa whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 102 kDa

    Exposure time: 3s

  • Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273), expandable thumbnail

    Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273)

    Transportin 1/MIP Western blot staining using rabbit Anti-Transportin 1/MIP antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Transportin 1/MIP antibody [EPR29606-11] (ab324273) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 50 µg

    Lane 2: Human lymph node tissue lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 102 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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