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AB127165

Anti-Transportin-2 antibody

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(2 Publications)

Rabbit Polyclonal Transportin-2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNPO2 aa 550-850.

View Alternative Names

Transportin-2, Karyopherin beta-2b, TNPO2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Transportin-2 antibody (AB127165)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Transportin-2 antibody (AB127165)

ab127165, at 1/500 dilution, detects paraformaldehyde-fixed HeLa, by Confocal immunofluorescence analysis (Olympus FV10i)

Western blot - Anti-Transportin-2 antibody (AB127165)
  • WB

Unknown

Western blot - Anti-Transportin-2 antibody (AB127165)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Transportin-2 antibody (ab127165) at 1/1000 dilution

Lane 1:

293T cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Predicted band size: 101 kDa,76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TNPO2 aa 550-850. The exact immunogen used to generate this antibody is proprietary information.

O14787

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Primary Antibodies

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Proteins & Peptides

AB114514

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SDS-PAGE - Recombinant Human Transportin 1/MIP protein (AB114514)

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Primary Antibodies

AB96551

Anti-MAP4K5 antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transportin-2 also known as TNPO2 facilitates the import of proteins into the nucleus by recognizing nuclear localization signals. This protein plays an important role in nuclear-cytoplasmic transport. Transportin-2 weighs approximately 97 kDa and is mainly expressed in various tissues including the brain and testis. The protein functions as part of the karyopherin family which are important for nucleocytoplasmic transport.
Biological function summary

Transportin-2 mediates the translocation of certain RNA-binding proteins and unspliced viral RNA between the cytoplasm and the nucleus. The protein often associates with the importin-β family forming a complex for efficient cargo delivery to the nucleus. Its specificity and binding affinity for different proteins highlight its importance in cellular function especially in regulating protein traffic necessary for gene expression and viral replication processes.

Pathways

Transportin-2 participates in cellular transport and protein sorting pathways. It functions closely with proteins like RanGTP which regulates the directionality of nuclear import. Additionally Transportin-2 is a part of the nuclear import pathway where it maintains the nuclear-cytoplasmic balance of several other nuclear transport proteins.

Transportin-2 links to several neurological disorders and some types of cancer. Alterations in its function and expression levels can affect protein import contributing to conditions like Huntington's disease due to disrupted nuclear transport processes. It also associates with proteins like Importin-α in mediating abnormal protein expression that might lead to cancerous growths. Understanding Transportin-2’s role in these diseases could offer insights for developing therapeutic advancements.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity).
See full target information TNPO2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer communications (London, England) 43:1117-1142 PubMed37658635

2023

JARID2 coordinates with the NuRD complex to facilitate breast tumorigenesis through response to adipocyte-derived leptin.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Yi Zeng,Xinhui Hao,Xin Wang,Jiaxiang Liu,Tianyang Gao,Mengdi Wang,Jingyao Zhang,Miaomiao Huo,Ting Hu,Tianyu Ma,Die Zhang,Xu Teng,Hefen Yu,Min Zhang,Baowen Yuan,Wei Huang,Yunkai Yang,Yan Wang

Cancer medicine 8:7299-7312 PubMed31605449

2019

TNPO2 operates downstream of DYNC1I1 and promotes gastric cancer cell proliferation and inhibits apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Libao Gong,Ti Wen,Zhi Li,Yizhe Wang,Jin Wang,Xiaofang Che,Yunpeng Liu,Xiujuan Qu
View all publications
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Product promise

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