AB240562

Anti-TRAP alpha/TRAPA antibody

Name: Anti-TRAP alpha/TRAPA antibody (ab240562) | Abcam
Brand: Abcam Limited
SKU: AB240562
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal TRAP alpha/TRAPA antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SSR1 aa 200 to C-terminus.

View Alternative Names

TRAPA, PSEC0262, SSR1, Translocon-associated protein subunit alpha, TRAP-alpha, Signal sequence receptor subunit alpha, SSR-alpha

1 Images

Supplier Data

Western blot - Anti-TRAP alpha/TRAPA antibody (AB240562)

All lanes:

Western blot - Anti-TRAP alpha/TRAPA antibody (ab240562) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 10 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Predicted band size: 32 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human SSR1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P43307

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP alpha also known as TRAPA or SSR1 is a protein with a mass of approximately 34 kDa. It locates within the endoplasmic reticulum (ER) membrane as a subunit of the translocon-associated protein complex. It has a role in maintaining the translocation of proteins across the endoplasmic reticulum membrane. TRAP alpha is widely expressed in various tissues reflecting its significant function in cellular processes.

Biological function summary

This protein serves as an integral component of the translocon complex facilitating protein transport into the ER. The TRAP complex includes several subunits that work together to ensure protein maturation and folding. TRAP alpha is especially essential for proper protein synthesis and quality control. It plays an important part in cellular homeostasis by ensuring that only correctly assembled proteins proceed to subsequent cellular pathways.

Pathways

TRAP alpha integrates into protein targeting and translocation pathways. It closely engages with the Sec61 complex another fundamental protein in translocation processes. TRAP alpha’s role is important in the secretory pathway which directs proteins destined for different organelles or secretion out of the cell. This pathway is interconnected with other cellular processes like the unfolded protein response ensuring cellular adaptation to stress conditions.

TRAP alpha associates with pathologies such as cystic fibrosis due to its involvement in protein processing. Mutations in proteins associated with TRAP alpha like CFTR can disrupt proper protein folding and cause disease. Additionally there are links between TRAP alpha and certain neurodegenerative conditions as defects in protein handling and folding within the ER can lead to cell death. Such associations also involve proteins like amyloid precursor protein underlining the importance of TRAP alpha in cellular health and disease prevention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.

See full target information SSR1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 24:100920 PubMed39880084

2025

Localized K63 Ubiquitin Signaling Is Regulated by VCP/p97 During Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Austin O Maduka,Sandhya Manohar,Matthew W Foster,Gustavo M Silva

Journal of Cancer 15:5786-5809 PubMed39308676

2024

Prognostic and Immunological Role of Cuproptosis-Related Gene MTF1 in Pan-Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhang,Sifen Wang,Hailin Tang,Renchun Lai,Qiaoting Cai,Yaorong Su,Hao Wu,Yongwen Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAP alpha/TRAPA antibody

Western blot - Anti-TRAP alpha/TRAPA antibody (AB240562)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise