AB248417

Anti-TRAP alpha/TRAPA antibody [EPR5603] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TRAP alpha/TRAPA antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

TRAPA, PSEC0262, SSR1, Translocon-associated protein subunit alpha, TRAP-alpha, Signal sequence receptor subunit alpha, SSR-alpha

2 Images

Flow Cytometry (Intracellular) - Anti-TRAP alpha/TRAPA antibody [EPR5603] - BSA and Azide free (AB248417)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TRAP alpha/TRAPA antibody [EPR5603] - BSA and Azide free (AB248417)

This data was developed using ab133238, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of TRAP alpha/TRAPA in permeabilized HepG2 cells, using ab133238 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Unknown

Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5603] - BSA and Azide free (AB248417)

This data was developed using ab133238, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5603] (<a href='/en-us/products/primary-antibodies/trap-alpha-trapa-antibody-epr5603-ab133238'>ab133238</a>) at 1/10000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

HL60 cell lysate at 10 µg

Lane 3:

T47D cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

Observed band size: 34 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5603

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab248417 is the carrier-free version of ab133238.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP alpha also known as TRAPA or SSR1 is a protein with a mass of approximately 34 kDa. It locates within the endoplasmic reticulum (ER) membrane as a subunit of the translocon-associated protein complex. It has a role in maintaining the translocation of proteins across the endoplasmic reticulum membrane. TRAP alpha is widely expressed in various tissues reflecting its significant function in cellular processes.

Biological function summary

This protein serves as an integral component of the translocon complex facilitating protein transport into the ER. The TRAP complex includes several subunits that work together to ensure protein maturation and folding. TRAP alpha is especially essential for proper protein synthesis and quality control. It plays an important part in cellular homeostasis by ensuring that only correctly assembled proteins proceed to subsequent cellular pathways.

Pathways

TRAP alpha integrates into protein targeting and translocation pathways. It closely engages with the Sec61 complex another fundamental protein in translocation processes. TRAP alpha’s role is important in the secretory pathway which directs proteins destined for different organelles or secretion out of the cell. This pathway is interconnected with other cellular processes like the unfolded protein response ensuring cellular adaptation to stress conditions.

TRAP alpha associates with pathologies such as cystic fibrosis due to its involvement in protein processing. Mutations in proteins associated with TRAP alpha like CFTR can disrupt proper protein folding and cause disease. Additionally there are links between TRAP alpha and certain neurodegenerative conditions as defects in protein handling and folding within the ER can lead to cell death. Such associations also involve proteins like amyloid precursor protein underlining the importance of TRAP alpha in cellular health and disease prevention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.

See full target information SSR1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAP alpha/TRAPA antibody [EPR5603] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-TRAP alpha/TRAPA antibody [EPR5603] - BSA and Azide free (AB248417)

Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5603] - BSA and Azide free (AB248417)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise