AB126775

Anti-TRAP alpha/TRAPA antibody [EPR5604]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal TRAP alpha/TRAPA antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

TRAPA, PSEC0262, SSR1, Translocon-associated protein subunit alpha, TRAP-alpha, Signal sequence receptor subunit alpha, SSR-alpha

2 Images

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Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (AB126775)

All lanes:

Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (ab126775) at 1/1000 dilution

Lane 1:

HL60 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 32 kDa

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OI-RD Scanning - Anti-TRAP alpha/TRAPA antibody [EPR5604] (AB126775)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TRAP alpha/TRAPA antibody [EPR5604] (AB126775)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-TRAP alpha/TRAPA antibody [EPR5604] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5604

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP alpha also known as TRAPA or SSR1 is a protein with a mass of approximately 34 kDa. It locates within the endoplasmic reticulum (ER) membrane as a subunit of the translocon-associated protein complex. It has a role in maintaining the translocation of proteins across the endoplasmic reticulum membrane. TRAP alpha is widely expressed in various tissues reflecting its significant function in cellular processes.

Biological function summary

This protein serves as an integral component of the translocon complex facilitating protein transport into the ER. The TRAP complex includes several subunits that work together to ensure protein maturation and folding. TRAP alpha is especially essential for proper protein synthesis and quality control. It plays an important part in cellular homeostasis by ensuring that only correctly assembled proteins proceed to subsequent cellular pathways.

Pathways

TRAP alpha integrates into protein targeting and translocation pathways. It closely engages with the Sec61 complex another fundamental protein in translocation processes. TRAP alpha’s role is important in the secretory pathway which directs proteins destined for different organelles or secretion out of the cell. This pathway is interconnected with other cellular processes like the unfolded protein response ensuring cellular adaptation to stress conditions.

TRAP alpha associates with pathologies such as cystic fibrosis due to its involvement in protein processing. Mutations in proteins associated with TRAP alpha like CFTR can disrupt proper protein folding and cause disease. Additionally there are links between TRAP alpha and certain neurodegenerative conditions as defects in protein handling and folding within the ER can lead to cell death. Such associations also involve proteins like amyloid precursor protein underlining the importance of TRAP alpha in cellular health and disease prevention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.

See full target information SSR1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

3 Biotech 11:258 PubMed33987074

2021

miR-9-5p promotes wear-particle-induced osteoclastogenesis through activation of the SIRT1/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhang,Weidong Zhao,Dongmei Bao,Kening Sun,Peng Li,Zhihui Gao,Zhidong Lu

International journal of molecular medicine 43:727-738 PubMed30535435

2018

MicroRNA‑100‑5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21.

Applications

Unspecified application

Species

Unspecified reactive species

Long Zhou,Han-Yi Song,Lin-Lin Gao,Li-Yu Yang,Shuai Mu,Qin Fu

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 47:2291-2306 PubMed29975944

2018

LncRNA LINC00311 Promotes the Proliferation and Differentiation of Osteoclasts in Osteoporotic Rats Through the Notch Signaling Pathway by Targeting DLL3.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Tian-Bao Luo,Long Liu,Zhi-Qiang Cui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAP alpha/TRAPA antibody [EPR5604]

Western blot - Anti-TRAP alpha/TRAPA antibody [EPR5604] (AB126775)

OI-RD Scanning - Anti-TRAP alpha/TRAPA antibody [EPR5604] (AB126775)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise