AB65854

Anti-TRAP/CD40L antibody

Name: Anti-TRAP/CD40L antibody (ab65854) | Abcam
Brand: Abcam Limited
SKU: AB65854
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(20 Publications)

Rabbit Polyclonal TRAP/CD40L antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human CD40LG.

View Alternative Names

CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody (AB65854)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody (AB65854)

TRAP/CD40L was detected in paraffin-embedded section of human mammary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65854 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

Unknown

Western blot - Anti-TRAP/CD40L antibody (AB65854)

All lanes:

Western blot - Anti-TRAP/CD40L antibody (ab65854) at 0.5 µg/mL

Lane 1:

MCF-7 whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

HMY2 whole cell lysate at 50 µg

Lane 5:

COLO320 whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP at 1/10000 dilution

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CD40LG. The exact immunogen used to generate this antibody is proprietary information.

P29965

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.

Biological function summary

The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.

Pathways

CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.

CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed : 1280226, PubMed : 31331973). Costimulates T-cell proliferation and cytokine production (PubMed : 8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed : 8617933). Induces the activation of NF-kappa-B (PubMed : 15067037, PubMed : 31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed : 15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).. CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5 : ITGB1 and ITGAV : ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

See full target information CD40LG

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:140 PubMed40312419

2025

Single-cell transcriptomic analysis reveals gut microbiota-immunotherapy synergy through modulating tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Minyuan Cao,Yun Deng,Qing Hao,Huayun Yan,Quan-Lin Wang,Chunyan Dong,Jing Wu,Yajiao He,Li-Bin Huang,Xuyang Xia,Yongchao Gao,Hai-Ning Chen,Wei-Han Zhang,Yan-Jing Zhang,Xiaozhen Zhuo,Lunzhi Dai,Hongbo Hu,Yong Peng,Feng Zhang,Zhaoqian Liu,Weihua Huang,Huiyuan Zhang,Li Yang,Yang Shu,Wei Zhang,Yan Zhang,Heng Xu

Journal of tissue engineering 15:20417314241306681 PubMed39712077

2024

Harnessing bone marrow mesenchymal stem cell-derived extracellular vesicles and biomimetic peptide WKYMVm in self-healing hydrogel for enhanced bone repair in femoral defects.

Applications

Unspecified application

Species

Unspecified reactive species

Keyu Luo,Yufei Jin,Baiyi Liu,Yingbo Wang,Yaoyao Liu,Si Qiu,Jianhua Zhao,Xiang Yin

Chinese medical journal 137:1316-1323 PubMed38738696

2024

Development and validation of a novel criterion of histologic healing in ulcerative colitis defined by inflammatory cell enumeration in lamina propria mucosa: A multicenter retrospective cohort in China.

Applications

Unspecified application

Species

Unspecified reactive species

Han Gao,Kangsheng Peng,Yadi Shi,Shenshen Zhu,Ruicong Sun,Chunjin Xu,Ping Liu,Zhi Pang,Lanxiang Zhu,Weichang Chen,Baisui Feng,Huili Wu,Guangxi Zhou,Mingsong Li,Junxiang Li,Baijing Ding,Zhanju Liu

International journal of molecular sciences 24: PubMed37958563

2023

CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Mohd I Nawaz,Ajmal Ahmad,Luna Dillemans,Mairaj Siddiquei,Eef Allegaert,Priscilla W Gikandi,Gert De Hertogh,Ghislain Opdenakker,Sofie Struyf

Cell death discovery 9:326 PubMed37658042

2023

Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Bi,Xiaofei Hou,Qiyang Shen,Zenan Liu,Xuehua Zhu,Lulin Ma,Jian Lu

Frontiers in immunology 14:1171308 PubMed37325657

2023

Involvement of CD40-CD40L and ICOS-ICOSL in the development of chronic rhinosinusitis by targeting eosinophils.

Applications

Unspecified application

Species

Unspecified reactive species

Aina Zhou,Chenxi Shi,Yuhui Fan,Yushuang Zheng,Jue Wang,Zhichen Liu,Huanxia Xie,Jisheng Liu,Qingqing Jiao

Journal of orthopaedic surgery and research 18:234 PubMed36949499

2023

Daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Lin Li,He-Dong Liu,Mao-Xian Ren,Zhi Zhou,Wen-Kai Jiang,Min Yang

Journal of orthopaedic surgery and research 17:423 PubMed36123704

2022

ZEB1 regulates bone metabolism in osteoporotic rats through inducing POLDIP2 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xianwei Zhu,Fei Yan,Lipeng Liu,Qun Huang

Journal of orthopaedic translation 36:132-144 PubMed36185580

2022

Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Ji,Yueyang Lu,Zhuoyi Ma,Ke Gan,Yan Liu,Yue Cheng,Junliang Xu,Shijia Liu,Yunke Guo,Shanhang Han,Zengyan Zhao,Hanmei Xu,Weiyan Qi

Scandinavian journal of immunology 96:e13169 PubMed35384009

2022

γ-Secretase inhibitors suppress IL-20-mediated osteoclastogenesis via Notch signalling and are affected by Notch2 in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Benyi Yang,Chaoran Fu,Yilin Wu,Yuanbo Liu,Zhen Zhang,Xin Chen,Dongle Wu,Ziqi Gan,Zhengyuan Chen,Yang Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TRAP/CD40L antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody (AB65854)

Western blot - Anti-TRAP/CD40L antibody (AB65854)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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