Rabbit Polyclonal TRAP/CD40L antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human CD40LG.
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- International journal of molecular sciences 24:2023CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed M Abu El-Asrar,Mohd I Nawaz,Ajmal Ahmad,Luna Dillemans,Mairaj Siddiquei,Eef Allegaert,Priscilla W Gikandi,Gert De Hertogh,Ghislain Opdenakker,Sofie StruyfPubMed 37958563
- Cell death discovery 9:3262023Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai Bi,Xiaofei Hou,Qiyang Shen,Zenan Liu,Xuehua Zhu,Lulin Ma,Jian LuPubMed 37658042
- Frontiers in immunology 14:11713082023Involvement of CD40-CD40L and ICOS-ICOSL in the development of chronic rhinosinusitis by targeting eosinophils.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAina Zhou,Chenxi Shi,Yuhui Fan,Yushuang Zheng,Jue Wang,Zhichen Liu,Huanxia Xie,Jisheng Liu,Qingqing JiaoPubMed 37325657
- Journal of orthopaedic translation 36:132-1442022Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Ji,Yueyang Lu,Zhuoyi Ma,Ke Gan,Yan Liu,Yue Cheng,Junliang Xu,Shijia Liu,Yunke Guo,Shanhang Han,Zengyan Zhao,Hanmei Xu,Weiyan QiPubMed 36185580
- Scandinavian journal of immunology 96:e131692022γ-Secretase inhibitors suppress IL-20-mediated osteoclastogenesis via Notch signalling and are affected by Notch2 in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenyi Yang,Chaoran Fu,Yilin Wu,Yuanbo Liu,Zhen Zhang,Xin Chen,Dongle Wu,Ziqi Gan,Zhengyuan Chen,Yang CaoPubMed 35384009
- Experimental and therapeutic medicine 23:2732022Role of HSP90α in osteoclast formation and osteoporosis development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianli Ma,Chen Yang,Huajian Zhong,Cheng Wang,Ke Zhang,Xiaoming Li,Jinhui Wu,Yang GaoPubMed 35251339
- Experimental and therapeutic medicine 22:6922021Overexpression of miRNA-22-3p attenuates osteoporosis by targeting MAPK14.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolin Jia,Ming Yang,Wei Hu,San CaiPubMed 33986857
- Translational cancer research 9:2482-24912020Denosumab inhibits MCF-7 cell line-induced spontaneous osteoclastogenesis via the RANKL/MALAT1/miR-124 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Feng,Donglai Wang,Jiangang Feng,Peng Guo,Cuizhi GengPubMed 35117607
- Experimental and therapeutic medicine 19:2188-21942020Raised CD40L expression attenuates drug resistance in Adriamycin-resistant THP-1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongxin Feng et. al.PubMed 32104283
- Journal of cellular physiology 235:1438-14522019MicroRNA-33 and SIRT1 influence the coronary thrombus burden in hyperglycemic STEMI patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNunzia D'Onofrio,Celestino Sardu,Pasquale Paolisso,Fabio Minicucci,Felice Gragnano,Franca Ferraraccio,Iacopo Panarese,Lucia Scisciola,Ciro Mauro,Maria Rosaria Rizzo,Gelsomina Mansueto,Federica Varavallo,Giuseppina Brunitto,Rosanna Caserta,Virginia Tirino,Gianpaolo Papaccio,Michelangela Barbieri,Giuseppe Paolisso,Maria Luisa Balestrieri,Raffaele MarfellaPubMed 31294459
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CD40LG. The exact immunogen used to generate this antibody is proprietary information. Database link P29965
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity). CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Alternative names
CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5
Recommended products
Rabbit Polyclonal TRAP/CD40L antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human CD40LG.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CD40LG. The exact immunogen used to generate this antibody is proprietary information. Database link P29965
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.
- Biological function summary
The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.
- Pathways
CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.
- Associated diseases and disorders
CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-TRAP/CD40L antibody (ab65854)
All lanes: Western blot - Anti-TRAP/CD40L antibody (ab65854) at 0.5 µg/mL
Lane 1: MCF-7 whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: HMY2 whole cell lysate at 50 µg
Lane 5: COLO320 whole cell lysate at 50 µg
SecondaryAll lanes: Goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody (ab65854)
TRAP/CD40L was detected in paraffin-embedded section of human mammary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65854 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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