AB259254

Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free

Name: Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254) | Abcam
Brand: Abcam Limited
SKU: AB259254

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(1 Publication)

Mouse Monoclonal TRAP/CD40L antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus.

View Alternative Names

CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5

4 Images

Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)

Flow Cytometric analysis of Jurkat cells staining TRAP/CD40L with ab257319 at 2μg/million cells, followed by a Goat anti-Mouse secondary antibody = blue. Isotype control = red.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257319).

Supplier Data

Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)

Immunohistochemical analysis of fomalin-fixed, paraffin-embedded human tonsil tissue staining TRAP/CD40L with ab257319 at 2μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257319).

Supplier Data

Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)

Immunohistochemical analysis of fomalin-fixed, paraffin-embedded human spleen tissue staining TRAP/CD40L with ab257319 at 2μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257319).

Supplier Data

Immunocytochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)

Immunocytochemical/Immunofluorescent analysis of paraformaldehyde-fixed Jurkat cells staining TRAP/CD40L with ab257319 at 2μg/ml, followed by a goat anti-mouse conjugated to CF488. Nuclei are stained with Reddot.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257319).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CD40LG/2761

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29965

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Perform antigen retrieval for 45 min at 95°C followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 30 minutes at room temperature." } } }

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Product details

ab259254 is the carrier-free version of ab257319.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.

Biological function summary

The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.

Pathways

CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.

CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed : 1280226, PubMed : 31331973). Costimulates T-cell proliferation and cytokine production (PubMed : 8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed : 8617933). Induces the activation of NF-kappa-B (PubMed : 15067037, PubMed : 31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed : 15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).. CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5 : ITGB1 and ITGAV : ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

See full target information CD40LG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 73:78 PubMed38554152

2024

Lipid droplets-related Perilipin-3: potential immune checkpoint and oncogene in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yijia He,Lingyun Liu,Yuexin Dong,Xiaoxin Zhang,Yuxian Song,Yue Jing,Yanhong Ni,Yi Wang,Zhiyong Wang,Liang Ding

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free

Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)

Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)

Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)

Immunocytochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise