Mouse Monoclonal TRAP/CD40L antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus.
Images
![Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--immunohistochemistry-img76889.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)")
![Immunocytochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--immunocytochemistry-img30614.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)")
![Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--flow-cytometry-img80897.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)")
![Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--immunohistochemistry-img6825.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)")
![Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--immunohistochemistry-img125527.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (AB259254)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29965
Reactivity data
Select an application| Flow Cyt | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform antigen retrieval for 45 min at 95°C followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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Target data
Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).CD40 ligand, soluble formActs as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Alternative names
CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, TRAP, TRAP, TNFSF5, CD40L, CD40LG
Recommended products
Mouse Monoclonal TRAP/CD40L antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus.
Alternative names
CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, TRAP, TRAP, TNFSF5, CD40L, CD40LG
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29965
- Carrier free
Yes
- Clone number
CD40LG/2761
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab259254 is the carrier-free version of ab257319.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
![Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--immunohistochemistry-img76889.jpg "Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)")
Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)
![Immunocytochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--immunocytochemistry-img30614.jpg "Immunocytochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)")
Immunocytochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)
Immunocytochemical/Immunofluorescent analysis of paraformaldehyde-fixed Jurkat cells staining TRAP/CD40L with ab257319 at 2μg/ml, followed by a goat anti-mouse conjugated to CF488. Nuclei are stained with Reddot.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257319).
![Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--flow-cytometry-img80897.jpg "Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)")
Flow Cytometry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)
Flow Cytometric analysis of Jurkat cells staining TRAP/CD40L with ab257319 at 2μg/million cells, followed by a Goat anti-Mouse secondary antibody = blue. Isotype control = red.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257319).
![Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--immunohistochemistry-img6825.jpg "Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)")
Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)
![Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254), expandable thumbnail](https://content.abcam.com/products/images/trap-cd40l-antibody-cd40lg-2761-bsa-and-azide-free-ab259254--immunohistochemistry-img125527.jpg "Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)")
Immunohistochemistry - Anti-TRAP/CD40L antibody [CD40LG/2761] - BSA and Azide free (ab259254)
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