AB52750

Anti-TRAP/CD40L antibody [EP462E]

RabMAb
Recombinant
20ul selling size
What is this?

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(19 Publications)

Rabbit Recombinant Monoclonal TRAP/CD40L antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 19 publications.

View Alternative Names

CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5

2 Images

Lab

Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750)

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 90 seconds
Lane 2 : 20 seconds

All lanes:

Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1 µg/mL

Lane 1:

Human lymph node tissue lysate at 20 µg

Lane 2:

Mouse lymph node tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 36 kDa

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Unknown

Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750)

All lanes:

Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1/5000 dilution

All lanes:

MOLT-4 (human lymphoblastic leukemia cell line) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 36 kDa

false

Carrier free

Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP462E

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with TRAP/CD40L.
Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.

Biological function summary

The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.

Pathways

CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.

CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed : 1280226, PubMed : 31331973). Costimulates T-cell proliferation and cytokine production (PubMed : 8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed : 8617933). Induces the activation of NF-kappa-B (PubMed : 15067037, PubMed : 31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed : 15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).. CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5 : ITGB1 and ITGAV : ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

See full target information CD40LG

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:348 PubMed40369573

2025

Engineered bone-targeting apoptotic vesicles as a minimally invasive nanotherapy for heterotopic ossification.

Applications

Unspecified application

Species

Unspecified reactive species

Like Tang,Yuchen Wang,Shihua Mao,Zhou Yu,Yitong Chen,Xiaoqiao Xu,Wenjin Cai,Kaichen Lai,Guoli Yang,Tingben Huang

Journal of cell communication and signaling 19:e70010 PubMed40191097

2025

Transcription factor GTF2I regulates osteoclast differentiation through mediating miR-134-5p and MAT2A expressions.

Applications

Unspecified application

Species

Unspecified reactive species

Lian Tang,Yanshi Liu,Jiyuan Yan,Lin Yuan,Zhaojun Wang,Zhong Li

Materials today. Bio 28:101225 PubMed39309162

2024

Nanoscale ZnO doping in prosthetic polymers mitigate wear particle-induced inflammation and osteolysis through inhibiting macrophage secretory autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuocheng Lyu,Xiangchao Meng,Fei Hu,Yuezhou Wu,Yurun Ding,Teng Long,Xinhua Qu,You Wang

Journal of orthopaedic surgery and research 19:128 PubMed38326867

2024

circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lian Tang,Lin Yuan,Jiyuan Yan,Jianhua Ge,Zhi Lian,Zhong Li

Journal of orthopaedic surgery and research 18:492 PubMed37434265

2023

CircZNF367 promotes osteoclast differentiation and osteoporosis by interacting with FUS to maintain CRY2 mRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Mingsi Deng,Zhengguang Wang,Jia Luo,Heng Cao,Yong Li,Liangjian Chen,Gengyan Liu

Environmental toxicology 38:2057-2068 PubMed37195267

2023

METTL14 represses osteoclast formation to ameliorate osteoporosis via enhancing GPX4 mRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Mingsi Deng,Jia Luo,Heng Cao,Yong Li,Liangjian Chen,Gengyan Liu

Cell death discovery 9:150 PubMed37156809

2023

GATA4-activated lncRNA MALAT1 promotes osteogenic differentiation through inhibiting NEDD4-mediated RUNX1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Xianzhe Huang,Shuo Jie,Wenzhao Li,Chan Liu

NPJ Regenerative medicine 8:6 PubMed36759627

2023

BMP-2 functional polypeptides relieve osteolysis via bi-regulating bone formation and resorption coupled with macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqian Wang,Yuan Xue,Yi Wang,Chang Liu,Sihan Hu,Huan Zhao,Qiaoli Gu,Huilin Yang,Lixin Huang,Xichao Zhou,Qin Shi

Frontiers in pharmacology 13:874952 PubMed35571086

2022

Baohuoside I Inhibits Osteoclastogenesis and Protects Against Ovariectomy-Induced Bone Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Min Ma,Ao-Yuan Fan,Zheng Liu,Li-Qing Yang,Jun-Ming Huang,Zhi-Ying Pang,Feng Yin

BMC musculoskeletal disorders 23:340 PubMed35397519

2022

MicroRNA-455-3p regulates proliferation and osteoclast differentiation of RAW264.7 cells by targeting PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Zhang,Liangming Wang,Nianlai Huang,Yiqiang Zheng,Liquan Cai,Qingfeng Ke,Shiqiang Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAP/CD40L antibody [EP462E]

Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750)

Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise