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AB52750

Anti-TRAP/CD40L antibody [EP462E]

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(9 Publications)

Rabbit Recombinant Monoclonal TRAP/CD40L antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 9 publications.

View Alternative Names

CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5

2 Images
Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750)
  • WB

Lab

Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750)

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 90 seconds
Lane 2 : 20 seconds

All lanes:

Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1 µg/mL

Lane 1:

Human lymph node tissue lysate at 20 µg

Lane 2:

Mouse lymph node tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 36 kDa

false

Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750)
  • WB

Unknown

Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750)

All lanes:

Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1/5000 dilution

All lanes:

MOLT-4 (human lymphoblastic leukemia cell line) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 36 kDa

false

  • Carrier free

    Anti-TRAP/CD40L antibody [EP462E] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP462E

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with TRAP/CD40L.
Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.

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Reactivity data

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AB196268

Human CD40L ELISA Kit (TNFSF5)

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.
Biological function summary

The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.

Pathways

CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.

CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed : 1280226, PubMed : 31331973). Costimulates T-cell proliferation and cytokine production (PubMed : 8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed : 8617933). Induces the activation of NF-kappa-B (PubMed : 15067037, PubMed : 31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed : 15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).. CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5 : ITGB1 and ITGAV : ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
See full target information CD40LG
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 13:874952 PubMed35571086

2022

Baohuoside I Inhibits Osteoclastogenesis and Protects Against Ovariectomy-Induced Bone Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Min Ma,Ao-Yuan Fan,Zheng Liu,Li-Qing Yang,Jun-Ming Huang,Zhi-Ying Pang,Feng Yin

BMC musculoskeletal disorders 23:340 PubMed35397519

2022

MicroRNA-455-3p regulates proliferation and osteoclast differentiation of RAW264.7 cells by targeting PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Zhang,Liangming Wang,Nianlai Huang,Yiqiang Zheng,Liquan Cai,Qingfeng Ke,Shiqiang Wu

Chinese medicine 17:18 PubMed35123541

2022

Toddalolactone protects against osteoarthritis by ameliorating chondrocyte inflammation and suppressing osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Xu,Song Xue,Tian Zhang,Xinmeng Jin,Cong Wang,Haiming Lu,Yiming Zhong,Hongjie Chen,Libo Zhu,Jinzhong Ma,Weilin Sang

Bioengineered 13:2685-2695 PubMed35001849

2022

3-hydroxy butyrate dehydrogenase 2 deficiency aggravates systemic lupus erythematosus progression in a mouse model by promoting CD40 ligand demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yang,Shihao Hou,Jingjing Zhao,Yepeng Li

Bioengineered 12:12677-12687 PubMed34933640

2021

MYC-mediated miR-320a affects receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast formation by regulating phosphatase and tensin homolog (PTEN).

Applications

Unspecified application

Species

Unspecified reactive species

Hao Chen,Shaoshuo Li,Heng Yin,Zhen Hua,Yang Shao,Jie Wei,Jianwei Wang

Frontiers in pharmacology 12:723988 PubMed34658862

2021

Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Yajun,Cui Jin,Gu Zhengrong,Fang Chao,Hu Yan,Weng Weizong,Li Xiaoqun,Zhou Qirong,Chen Huiwen,Zhang Hao,Guo Jiawei,Zhuang Xinchen,Sheng Shihao,Wang Sicheng,Chen Xiao,Su Jiacan

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 40:1256-1273 PubMed31272312

2019

Prevention of brain damage after traumatic brain injury by pharmacological enhancement of KCNQ (Kv7, "M-type") K currents in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio A Vigil,Eda Bozdemir,Vladislav Bugay,Sang H Chun,MaryAnn Hobbs,Isamar Sanchez,Shayne D Hastings,Rafael J Veraza,Deborah M Holstein,Shane M Sprague,Chase M Carver,Jose E Cavazos,Robert Brenner,James D Lechleiter,Mark S Shapiro

Scientific reports 7:202 PubMed28303002

2017

Reciprocal regulation of γ-globin expression by exo-miRNAs: Relevance to γ-globin silencing in β-thalassemia major.

Applications

Flow Cyt

Species

Human

Kuo-Ting Sun,Yu-Nan Huang,Kalaiselvi Palanisamy,Shih-Sheng Chang,I-Kuan Wang,Kang-Hsi Wu,Ping Chen,Ching-Tien Peng,Chi-Yuan Li

Experimental and therapeutic medicine 12:3242-3248 PubMed27882144

2016

Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis.

Applications

IHC-P

Species

Rabbit

Xuan Wang,Yue-Cheng Hu,Ru-Yan Zhang,Dong-Xia Jin,Yuan Jiang,He-Nan Zhang,Hong-Liang Cong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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