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Rabbit Recombinant Monoclonal TRAP/CD40L antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 9 publications.

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Images

Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750), expandable thumbnail
  • Western blot - Anti-TRAP/CD40L antibody [EP462E] (AB52750), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWB
Human
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000

Notes

-

Species

Human

Dilution info

1/5000

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).CD40 ligand, soluble formActs as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TRAP/CD40L antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 9 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP462E

Purification technique

Affinity purification Protein A

Specificity

This antibody reacts with TRAP/CD40L.
Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750), expandable thumbnail

    Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750)

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 90 seconds
    Lane 2: 20 seconds

    All lanes: Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1 µg/mL

    Lane 1: Human lymph node tissue lysate at 20 µg

    Lane 2: Mouse lymph node tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 29 kDa

    Observed band size: 36 kDa

  • Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750), expandable thumbnail

    Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750)

    All lanes: Western blot - Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1/5000 dilution

    All lanes: MOLT-4 (human lymphoblastic leukemia cell line) cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 29 kDa

    Observed band size: 36 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com