AB99894

Anti-TRAP/CD40L antibody [MR-1]

Name: Anti-TRAP/CD40L antibody [MR-1] (ab99894) | Abcam
Brand: Abcam Limited
SKU: AB99894
Price: 495 USD
Availability: InStock

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(2 Publications)

Armenian hamster Monoclonal TRAP/CD40L antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Cd40lg protein.

View Alternative Names

CD154, Cd40l, Tnfsf5, Cd40lg, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, TRAP

1 Images

Flow Cytometry - Anti-TRAP/CD40L antibody [MR-1] (AB99894)

Flow Cyt

Unknown

Flow Cytometry - Anti-TRAP/CD40L antibody [MR-1] (AB99894)

Flow Cytometry analysis of CD154 positive cells (red) from mouse CD154 negative cells (black) using ab99894 at 3 μg/ml.

519 FITC

FITC Anti-TRAP/CD40L antibody [MR-1]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

MR-1

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd40lg protein. The exact immunogen used to generate this antibody is proprietary information.

P27548

Specificity

This antibody detects mouse TRAP/CD40L/CD154. a 39 kDa cell surface glycoprotein expressed predominantly on activated CD4+ lymphocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "3 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

Functional Application: Blocking T cell costimulation in vivo.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from cell culture supernatant (> 95% by SDS-PAGE)

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.

Biological function summary

The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.

Pathways

CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.

CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity). Costimulates T-cell proliferation and cytokine production (By similarity). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity). Induces the activation of NF-kappa-B (By similarity). Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed : 1374165). Involved in immunoglobulin class switching (PubMed : 1374165).. CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5 : ITGB1 and ITGAV : ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

See full target information Cd40lg

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 8:117 PubMed35292660

2022

MYC inhibition reprograms tumor immune microenvironment by recruiting T lymphocytes and activating the CD40/CD40L system in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo Jiang,Qianfeng Zhang,Yong Fan,Jia Li,Jitao Zhang,Wentao Wang,Jinzhu Fan,Yunshan Guo,Shichang Liu,Dingjun Hao,Yongxiang Wang,Lei Wang,Lequn Shan

Oncotarget 7:47891-47903 PubMed27374105

2016

The essential role of GATA transcription factors in adult murine prostate.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Xiao,Qin Feng,Zheng Zhang,Fen Wang,John P Lydon,Michael M Ittmann,Li Xin,Nicholas Mitsiades,Bin He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAP/CD40L antibody [MR-1]

Flow Cytometry - Anti-TRAP/CD40L antibody [MR-1] (AB99894)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise