Anti-TRAP1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRAP1 antibody (AB151239)

Immunofluorescence analysis of ICC-IF HeLa cells fixed in ice-cold MeOH for 5 min labelling TRAP1 protein at mitochondria using ab151239 at a 1/1000 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).
Scale bar = 10 µM

• WB

Unknown

Western blot - Anti-TRAP1 antibody (AB151239)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TRAP1 antibody (ab151239) at 1/1500 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 80 kDa

false

• WB

Lab

Western blot - Anti-TRAP1 antibody (AB151239)

All lanes:

Western blot - Anti-TRAP1 antibody (ab151239) at 1/500 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

TRAP1 Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-TRAP1 antibody (AB151239)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-TRAP1 antibody (ab151239) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-TRAP1 antibody (AB151239)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-TRAP1 antibody (ab151239) at 1/1000 dilution

All lanes:

Mouse muscle tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

false