Anti-TRAP1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody (AB226401)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for TRAP1 using ab226401 at 1/1000 dilution in immunohistochemical analysis.

Detection : DAB staining.

• WB

Supplier Data

Western blot - Anti-TRAP1 antibody (AB226401)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-TRAP1 antibody (ab226401) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 80 kDa

true

Exposure time: 10s

• IP

Supplier Data

Immunoprecipitation - Anti-TRAP1 antibody (AB226401)

Hsp75 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226401 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226401 at 0.4 μg/ml.

Lane 1 : ab226401 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TRAP1 antibody (ab226401)

Predicted band size: 80 kDa

false