Rabbit Polyclonal TRAP100/MED24 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MED24 aa 800-850.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MED24 aa 800-850. The exact immunogen used to generate this antibody is proprietary information. Database link O75448
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alternative names
ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100, MED24, Mediator of RNA polymerase II transcription subunit 24, Activator-recruited cofactor 100 kDa component, Cofactor required for Sp1 transcriptional activation subunit 4, Mediator complex subunit 24, Thyroid hormone receptor-associated protein 4, Thyroid hormone receptor-associated protein complex 100 kDa component, Vitamin D3 receptor-interacting protein complex 100 kDa component, CRSP complex subunit 4, Trap100, hTRAP100
Recommended products
Rabbit Polyclonal TRAP100/MED24 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MED24 aa 800-850.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MED24 aa 800-850. The exact immunogen used to generate this antibody is proprietary information. Database link O75448
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAP100 also known as MED24 is a subunit of the Mediator complex with an approximate mass of 100 kDa. It plays a significant role in transcription regulation by serving as a bridge between transcription factors and RNA polymerase II. TRAP100/MED24 is ubiquitously expressed in various tissues reflecting its central role in gene expression. It interacts with several other subunits within the Mediator complex to facilitate RNA transcription initiation.
- Biological function summary
The TRAP100/MED24 protein engages in cellular processes by working as part of the Mediator complex. This complex is essential in bridging enhancer-bound transcription factors with the basal transcriptional machinery. It plays a significant role in regulating gene expression tied to growth and development. The Mediator complex consisting of over 30 subunits including TRAP100/MED24 acts as a coactivator impacting the transcription of messenger RNA in eukaryotic cells.
- Pathways
This protein applies its function in transcription regulation and is essential in both Wnt and Hedgehog signaling pathways. It aids the recruitment of RNA polymerase II which is important for the initiation phase in these pathways. Interactions with proteins such as β-catenin and Gli proteins reveal the involvement of TRAP100/MED24 in transcriptional regulation downstream of these pathways influencing gene networks involved in cell fate determination and tissue homeostasis.
- Associated diseases and disorders
Defective TRAP100/MED24 function links to conditions such as cancer and neurodevelopmental disorders. TRAP100/MED24 mutations can affect the transcriptional regulation cascades leading to aberrant cellular proliferation and differentiation seen in cancer. Neurodevelopmental disorders can arise from disrupted developmental processes controlled by Mediator complex components including MED12-related syndromes. Understanding connections between TRAP100/MED24 and related proteins such as β-catenin and Gli proteins aids in exploring therapeutic interventions for associated diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-TRAP100/MED24 antibody (ab194951)
All lanes: Western blot - Anti-TRAP100/MED24 antibody (ab194951) at 1/500 dilution
All lanes: 293 cell extract
Predicted band size: 110 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP100/MED24 antibody (ab194951)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Brain, Cortex tissue labeling TRAP100/MED24 with ab194951 at 5 μg/ml.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com