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AB194951

Anti-TRAP100/MED24 antibody

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(1 Publication)

Rabbit Polyclonal TRAP100/MED24 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MED24 aa 800-850.

View Alternative Names

ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100, MED24, Mediator of RNA polymerase II transcription subunit 24, Activator-recruited cofactor 100 kDa component, Cofactor required for Sp1 transcriptional activation subunit 4, Mediator complex subunit 24, Thyroid hormone receptor-associated protein 4, Thyroid hormone receptor-associated protein complex 100 kDa component, Vitamin D3 receptor-interacting protein complex 100 kDa component, CRSP complex subunit 4, Trap100, hTRAP100

2 Images
Western blot - Anti-TRAP100/MED24 antibody (AB194951)
  • WB

Supplier Data

Western blot - Anti-TRAP100/MED24 antibody (AB194951)

All lanes:

Western blot - Anti-TRAP100/MED24 antibody (ab194951) at 1/500 dilution

All lanes:

293 cell extract

Predicted band size: 110 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP100/MED24 antibody (AB194951)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP100/MED24 antibody (AB194951)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Brain, Cortex tissue labeling TRAP100/MED24 with ab194951 at 5 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MED24 aa 800-850. The exact immunogen used to generate this antibody is proprietary information.

O75448

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZAP70 (phospho Y493) antibody (AB194800)

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Primary Antibodies

AB193624

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Primary Antibodies

AB194747

Anti-VASP (phospho S239) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S239) antibody (AB194747)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP100 also known as MED24 is a subunit of the Mediator complex with an approximate mass of 100 kDa. It plays a significant role in transcription regulation by serving as a bridge between transcription factors and RNA polymerase II. TRAP100/MED24 is ubiquitously expressed in various tissues reflecting its central role in gene expression. It interacts with several other subunits within the Mediator complex to facilitate RNA transcription initiation.
Biological function summary

The TRAP100/MED24 protein engages in cellular processes by working as part of the Mediator complex. This complex is essential in bridging enhancer-bound transcription factors with the basal transcriptional machinery. It plays a significant role in regulating gene expression tied to growth and development. The Mediator complex consisting of over 30 subunits including TRAP100/MED24 acts as a coactivator impacting the transcription of messenger RNA in eukaryotic cells.

Pathways

This protein applies its function in transcription regulation and is essential in both Wnt and Hedgehog signaling pathways. It aids the recruitment of RNA polymerase II which is important for the initiation phase in these pathways. Interactions with proteins such as β-catenin and Gli proteins reveal the involvement of TRAP100/MED24 in transcriptional regulation downstream of these pathways influencing gene networks involved in cell fate determination and tissue homeostasis.

Defective TRAP100/MED24 function links to conditions such as cancer and neurodevelopmental disorders. TRAP100/MED24 mutations can affect the transcriptional regulation cascades leading to aberrant cellular proliferation and differentiation seen in cancer. Neurodevelopmental disorders can arise from disrupted developmental processes controlled by Mediator complex components including MED12-related syndromes. Understanding connections between TRAP100/MED24 and related proteins such as β-catenin and Gli proteins aids in exploring therapeutic interventions for associated diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED24
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Poultry science 103:103912 PubMed38943808

2024

Follicular fluid-derived exosomal HMOX1 promotes granulosa cell ferroptosis involved in follicular atresia in geese (Anser cygnoides).

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Youluan Jiang,Xiaoqian Dong,Shuwen Luo,Guoyu Jiao,Kaiqi Weng,Qiang Bao,Yang Zhang,Wanwipa Vongsangnak,Guohong Chen,Qi Xu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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