Rabbit Recombinant Monoclonal TRAP220/MED1 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
Images
![Sandwich ELISA - Anti-TRAP220/MED1 antibody [EPR24656-67] - BSA and Azide free (Detector) (AB303637), expandable thumbnail](https://content.abcam.com/products/images/trap220-med1-antibody-epr24656-67-bsa-and-azide-free-detector-ab303637--sandwich-elisa-img403608.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-TRAP220/MED1 antibody [EPR24656-67] - BSA and Azide free (Detector) (AB303637)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).
Alternative names
Mediator of RNA polymerase II transcription subunit 1, Activator-recruited cofactor 205 kDa component, Mediator complex subunit 1, Peroxisome proliferator-activated receptor-binding protein, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor-interacting protein 2, Vitamin D receptor-interacting protein complex component DRIP205, p53 regulatory protein RB18A, ARC205, PBP, PPAR-binding protein, Trap220, TR-interacting protein 2, TRIP-2, CRSP200, TRIP2, TRAP220, RB18A, MED1, PPARGBP, PPARBP, ARC205, CRSP1, PBP, DRIP205, DRIP230
Recommended products
Rabbit Recombinant Monoclonal TRAP220/MED1 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
Alternative names
Mediator of RNA polymerase II transcription subunit 1, Activator-recruited cofactor 205 kDa component, Mediator complex subunit 1, Peroxisome proliferator-activated receptor-binding protein, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor-interacting protein 2, Vitamin D receptor-interacting protein complex component DRIP205, p53 regulatory protein RB18A, ARC205, PBP, PPAR-binding protein, Trap220, TR-interacting protein 2, TRIP-2, CRSP200, TRIP2, TRAP220, RB18A, MED1, PPARGBP, PPARBP, ARC205, CRSP1, PBP, DRIP205, DRIP230
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR24656-67
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- Activity summary
TRAP220 also known as MED1 is a protein with a molecular mass of approximately 220 kDa. It plays an important role as a component of the mediator complex which is important for transcription regulation. The expression of TRAP220/MED1 is found in various tissues with higher levels observed in liver lung and heart tissues. This protein acts mechanistically as a bridge between gene-specific transcription factors and RNA polymerase II facilitating the assembly of the pre-initiation complex.
- Biological function summary
TRAP220/MED1 functions within the larger mediator complex a multi-protein coactivator essential for gene transcription by RNA polymerase II. It plays an important role in nuclear receptor signaling facilitating the transcriptional activation by various nuclear hormone receptors. MED1 interacts directly with these receptors through its various domains allowing the transcription of target genes responsive to hormonal signals.
- Pathways
TRAP220/MED1 contributes significantly to transcriptional activation pathways involving nuclear receptors such as the PPAR (Peroxisome Proliferator-Activated Receptor) and thyroid hormone receptor pathways. It closely interacts with other proteins in these pathways including MED12 and MED14 which form integral parts of the mediator complex's function. Its involvement in these pathways highlights its critical role in lipid metabolism and energy homeostasis.
- Associated diseases and disorders
TRAP220/MED1 is associated with metabolic and proliferative diseases. Its altered expression or mutations can lead to conditions such as obesity and cancer. For instance aberrant MED1 activity or expression levels have connections to breast cancer development through its role in estrogen receptor-mediated gene expression. This correlation emphasizes the protein's potential impact in various pathophysiological contexts driving ongoing research into MED1 as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-TRAP220/MED1 antibody [EPR24656-67] - BSA and Azide free (Detector) (ab303637), expandable thumbnail](https://content.abcam.com/products/images/trap220-med1-antibody-epr24656-67-bsa-and-azide-free-detector-ab303637--sandwich-elisa-img403608.jpg "Sandwich ELISA - Anti-TRAP220/MED1 antibody [EPR24656-67] - BSA and Azide free (Detector) (ab303637)")
Sandwich ELISA - Anti-TRAP220/MED1 antibody [EPR24656-67] - BSA and Azide free (Detector) (ab303637)
Sandwich ELISA of TRAP220/MED1 Antibody Pair - BSA and Azide free ab303635 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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