AB181103

Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal TRAP220/MED1 phospho T1457 antibody. Suitable for Dot, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ARC205, CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2, MED1, Mediator of RNA polymerase II transcription subunit 1, Activator-recruited cofactor 205 kDa component, Mediator complex subunit 1, Peroxisome proliferator-activated receptor-binding protein, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor-interacting protein 2, Vitamin D receptor-interacting protein complex component DRIP205, p53 regulatory protein RB18A, PPAR-binding protein, Trap220, TR-interacting protein 2, TRIP-2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (AB181103)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (AB181103)

Immunohistochemical analysis of paraffin-embedded sections of Human pancreas tissue labeling TRAP220/MED1 (phospho T1457) using ab181103 at a 1/250 dilution followed by a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (AB181103)

Immunohistochemical analysis of paraffin-embedded sections of Human thyroid gland carcinoma tissue labeling TRAP220/MED1 (phospho T1457) using ab181103 at a 1/250 dilution followed by a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (AB181103)

All lanes:

Western blot - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (ab181103) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 168 kDa

false

Unknown

Dot Blot - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (AB181103)

Dot blot analysis of TRAP220/MED1 (pT1457) peptide (Lane 1) TRAP220/MED1 non-phospho peptide (Lane 2) labelling TRAP220/MED1 (pT1457) with ab181103 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

Carrier free

Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13269

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Dot, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP220 also known as MED1 is a protein with a molecular mass of approximately 220 kDa. It plays an important role as a component of the mediator complex which is important for transcription regulation. The expression of TRAP220/MED1 is found in various tissues with higher levels observed in liver lung and heart tissues. This protein acts mechanistically as a bridge between gene-specific transcription factors and RNA polymerase II facilitating the assembly of the pre-initiation complex.

Biological function summary

TRAP220/MED1 functions within the larger mediator complex a multi-protein coactivator essential for gene transcription by RNA polymerase II. It plays an important role in nuclear receptor signaling facilitating the transcriptional activation by various nuclear hormone receptors. MED1 interacts directly with these receptors through its various domains allowing the transcription of target genes responsive to hormonal signals.

Pathways

TRAP220/MED1 contributes significantly to transcriptional activation pathways involving nuclear receptors such as the PPAR (Peroxisome Proliferator-Activated Receptor) and thyroid hormone receptor pathways. It closely interacts with other proteins in these pathways including MED12 and MED14 which form integral parts of the mediator complex's function. Its involvement in these pathways highlights its critical role in lipid metabolism and energy homeostasis.

TRAP220/MED1 is associated with metabolic and proliferative diseases. Its altered expression or mutations can lead to conditions such as obesity and cancer. For instance aberrant MED1 activity or expression levels have connections to breast cancer development through its role in estrogen receptor-mediated gene expression. This correlation emphasizes the protein's potential impact in various pathophysiological contexts driving ongoing research into MED1 as a therapeutic target.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed : 10406464, PubMed : 11867769, PubMed : 12037571, PubMed : 12218053, PubMed : 12556447, PubMed : 14636573, PubMed : 15340084, PubMed : 15471764, PubMed : 15989967, PubMed : 16574658, PubMed : 9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed : 24245781).

See full target information MED1 phospho T1457

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 12:e0168960 PubMed28045951

2017

An Experimental Analysis of the Molecular Effects of Trastuzumab (Herceptin) and Fulvestrant (Falsodex), as Single Agents or in Combination, on Human HR+/HER2+ Breast Cancer Cell Lines and Mouse Tumor Xenografts.

Applications

Species

Unspecified reactive species

Qing Chen,Ziyi Weng,Yunshu Lu,Yijun Jia,Longlong Ding,Fang Bai,Meixin Ge,Qing Lin,Kejin Wu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (AB181103)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (AB181103)

Western blot - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (AB181103)

Dot Blot - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (AB181103)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise