JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155593

Anti-TRAP80 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal TRAP80 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MED17 aa 400 to C-terminus.

View Alternative Names

ARC77, CRSP6, DRIP77, DRIP80, TRAP80, MED17, Mediator of RNA polymerase II transcription subunit 17, Activator-recruited cofactor 77 kDa component, Cofactor required for Sp1 transcriptional activation subunit 6, Mediator complex subunit 17, Thyroid hormone receptor-associated protein complex 80 kDa component, Transcriptional coactivator CRSP77, Vitamin D3 receptor-interacting protein complex 80 kDa component, CRSP complex subunit 6, Trap80

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TRAP80 antibody (AB155593)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRAP80 antibody (AB155593)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling TRAP80 with ab155593 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP80 antibody (AB155593)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP80 antibody (AB155593)

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft tissue labeling TRAP80 with ab155593 at 1/500 dilution.

Western blot - Anti-TRAP80 antibody (AB155593)
  • WB

Unknown

Western blot - Anti-TRAP80 antibody (AB155593)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TRAP80 antibody (ab155593) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MED17 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NVC6

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocolusing 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAP80 also known as Mediator complex subunit 13 (MED13) or Thyroid Hormone Receptor-Associated Protein 80 kDa (THRAP80) plays an essential role in transcription regulation. It is a part of the Mediator complex and has a molecular mass of approximately 80 kDa. TRAP80 functions as a bridge connecting transcriptional activators bound at enhancer regions to the RNA polymerase II machinery enabling transcription initiation. Expression of TRAP80 occurs in various human tissues including heart brain and liver where it mediates gene expression responsive to hormonal signals.
Biological function summary

TRAP80 is involved in regulating gene expression by modifying the chromatin structure which impacts transcription by making specific genes accessible or inaccessible to RNA polymerase II. As a part of the Mediator complex TRAP80 participates in facilitating interactions between enhancers and promoters influencing transcription rates. Through these actions TRAP80 impacts processes like cell growth differentiation and response to external signals and it maintains proper cellular function by regulating gene networks.

Pathways

TRAP80 notably engages in the Wnt signaling pathway and thyroid hormone signaling pathway where it helps modulate transcriptional responses by activating or repressing target genes. In the Wnt pathway TRAP80 works with beta-catenin an important mediator to regulate target gene expression linked with cellular proliferation and differentiation. In the thyroid hormone pathway TRAP80 works in concert with thyroid hormone receptors to mediate the biological effects of thyroid hormones influencing metabolism and development.

TRAP80 has associations with cancer and congenital heart defects. TRAP80's involvement in cancer relates to its role in misregulated transcription pathways that drive oncogenesis. It impacts cancers by facilitating mutations that change normal cell growth and differentiation signals. Additionally in congenital heart defects disturbances in the pathways involving TRAP80 can lead to improper cardiac development. MED13 as a part of the same complex has been studied in connection with these diseases highlighting the importance of the Mediator complex in disease pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED17
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:15615 PubMed28561026

2017

R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Philippe Cloutier,Christian Poitras,Mathieu Durand,Omid Hekmat,Émilie Fiola-Masson,Annie Bouchard,Denis Faubert,Benoit Chabot,Benoit Coulombe
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB216593

Anti-TRAP80 antibody

Western blot - Anti-TRAP80 antibody (AB216593)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB72141

Anti-CRSP2 / MED14 antibody

Immunoprecipitation - Anti-CRSP2 / MED14 antibody (AB72141)

  • 0
  • 0
Primary Antibodies

AB252881

Anti-GFP antibody [3H9]

BOND RX™ Validated|Recombinant|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (AB252881)

  • 0
  • 0
Primary Antibodies

AB5131

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody

ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

  • 4
  • 48
Primary Antibodies

AB22758

Anti-Nucleolin antibody

BOND RX™ Validated

Western blot - Anti-Nucleolin antibody (AB22758)

  • 4
  • 8

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com