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AB247942

Anti-TRAPPC2 antibody [EPR3401] - BSA and Azide free

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Rabbit Recombinant Monoclonal TRAPPC2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

SEDL, TRAPPC2, Trafficking protein particle complex subunit 2, Sedlin

1 Images
Western blot - Anti-TRAPPC2 antibody [EPR3401] - BSA and Azide free (AB247942)
  • WB

Unknown

Western blot - Anti-TRAPPC2 antibody [EPR3401] - BSA and Azide free (AB247942)

This data was developed using ab111848, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TRAPPC2 antibody [EPR3401] (<a href='/en-us/products/primary-antibodies/trappc2-antibody-epr3401-ab111848'>ab111848</a>) at 1/1000 dilution

Lane 1:

Human spleen cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

Daudi cell lysate at 10 µg

Predicted band size: 16 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3401

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247942 is the carrier-free version of ab111848.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAPPC2 also known as sedlin is a component of the transport protein particle (TRAPP) complex with a molecular mass of approximately 17 kDa. It is a small protein that has a significant role in vesicular trafficking within cells. TRAPPC2 is expressed widely in human tissues with notable levels in the testis skeletal muscle and heart. This protein participates in the regulation of intracellular transport processes by assembling into multi-subunit complexes that facilitate the movement of vesicles.
Biological function summary

TRAPPC2 plays a role in the formation and function of the TRAPP complex which is fundamental for intracellular vesicle trafficking. The TRAPP complex exists in different forms and participates in the tethering of vesicles to target membranes. TRAPPC2’s involvement in this complex influences ER-Golgi transportation impacting how proteins and lipids move inside cells. By working as part of the TRAPP complex TRAPPC2 contributes to the maintenance of cellular homeostasis by controlling the flow and direction of cargo.

Pathways

TRAPPC2 is involved in the secretory pathway which is essential for protein sorting and transport. It interacts with other TRAPP subunits such as TRAPPC3 and TRAPPC9 to ensure effective vesicle fusion and trafficking processes. In addition to the secretory pathway TRAPPC2 is engaged in the autophagy pathway. Through its participation in these pathways TRAPPC2 affects how cells manage and recycle cellular components under varying conditions.

Mutations in TRAPPC2 are linked to X-linked spondyloepiphyseal dysplasia tarda a skeletal disorder affecting bone growth and development. The deficiency of TRAPPC2 disrupts normal vesicular trafficking ultimately leading to the symptoms observed in this disorder. TRAPPC2’s interactions with proteins such as TRAPPC3 and TRAPPC9 through these pathways underline its significance in maintaining cellular functions that when altered result in specific pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Prevents transcriptional repression and induction of cell death by ENO1 (By similarity). May play a role in vesicular transport from endoplasmic reticulum to Golgi.
See full target information TRAPPC2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com