AB250532

Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TRAX antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples.

View Alternative Names

TRAX, TSNAX, Translin-associated protein X, Translin-associated factor X

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)

This data was developed using ab181862, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling TRAX with ab181862 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)

This data was developed using ab181862, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human testis lysate immunoprecipitated using ab181862 at 1/60 dilution. Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TRAX antibody [EPR13929(B)] (<a href='/en-us/products/primary-antibodies/trax-antibody-epr13929b-ab181862'>ab181862</a>)

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)

This data was developed using ab181862, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TRAX antibody [EPR13929(B)] (<a href='/en-us/products/primary-antibodies/trax-antibody-epr13929b-ab181862'>ab181862</a>) at 1/5000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)

This data was developed using ab181862, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TRAX antibody [EPR13929(B)] (<a href='/en-us/products/primary-antibodies/trax-antibody-epr13929b-ab181862'>ab181862</a>) at 1/20000 dilution

Lane 1:

Mouse testis lysate at 20 µg

Lane 2:

Rat testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 106 kDa,24 kDa,33 kDa,52 kDa

Observed band size: 110 kDa,25 kDa

false

Unconjugated

Anti-TRAX antibody [EPR13929(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13929(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab250532 is the carrier-free version of ab181862.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein TRAX also known as Translin-associated factor X functions mechanically as an RNA-binding protein. It plays a role in binding to specific RNA sequences and is involved in RNA transport and stability. The protein has an approximate molecular weight of 31 kDa. TRAX is expressed in various tissues including brain testis and hematopoietic tissues indicating its involvement in diverse biological processes.

Biological function summary

TRAX interacts with Translin to form a heteromeric complex that is involved in mRNA regulation. The complex participates in the regulation of neural development and synaptic plasticity by stabilizing mRNA and modulating its translation. TRAX’s involvement in RNA processing suggests it may have implications in cellular growth and response to stress.

Pathways

TRAX participates in the RNA regulatory network including the MAPK signaling pathway. It influences stress responses and cell survival by associating with proteins like Translin. This pathway coordination ensures cells can adapt to environmental changes efficiently important in maintaining neuronal health.

TRAX has links to neurodegenerative diseases and certain cancers. Alterations in TRAX expression or function can contribute to conditions such as schizophrenia. Its interaction with proteins in disease contexts including Translin suggests that misregulation of the TRAX-Translin complex may play a role in the pathology by impairing RNA transport and stability disrupting cellular functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts in combination with TSN as an endonuclease involved in the activation of the RNA-induced silencing complex (RISC). Possible role in spermatogenesis.

See full target information TSNAX

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)

Immunoprecipitation - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)

Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)

Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise