Rabbit Recombinant Monoclonal TRAX antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Images
![Immunoprecipitation - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532), expandable thumbnail](https://content.abcam.com/products/images/trax-antibody-epr13929b-bsa-and-azide-free-ab250532--immunoprecipitation-img74488.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)")
![Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532), expandable thumbnail](https://content.abcam.com/products/images/trax-antibody-epr13929b-bsa-and-azide-free-ab250532--western-blot-img58065.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)")
![Immunocytochemistry/ Immunofluorescence - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532), expandable thumbnail](https://content.abcam.com/products/images/trax-antibody-epr13929b-bsa-and-azide-free-ab250532--immunocytochemistry-immunofluorescence-img62879.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)")
![Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532), expandable thumbnail](https://content.abcam.com/products/images/trax-antibody-epr13929b-bsa-and-azide-free-ab250532--western-blot-img61677.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (AB250532)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Expected |
| Mouse | Expected | Expected | Expected |
| Rat | Expected | Expected | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Acts in combination with TSN as an endonuclease involved in the activation of the RNA-induced silencing complex (RISC). Possible role in spermatogenesis.
Alternative names
TRAX, TSNAX, Translin-associated protein X, Translin-associated factor X
Recommended products
Rabbit Recombinant Monoclonal TRAX antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13929(B)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250532 is the carrier-free version of Anti-TRAX antibody [EPR13929(B)] ab181862.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein TRAX also known as Translin-associated factor X functions mechanically as an RNA-binding protein. It plays a role in binding to specific RNA sequences and is involved in RNA transport and stability. The protein has an approximate molecular weight of 31 kDa. TRAX is expressed in various tissues including brain testis and hematopoietic tissues indicating its involvement in diverse biological processes.
- Biological function summary
TRAX interacts with Translin to form a heteromeric complex that is involved in mRNA regulation. The complex participates in the regulation of neural development and synaptic plasticity by stabilizing mRNA and modulating its translation. TRAX’s involvement in RNA processing suggests it may have implications in cellular growth and response to stress.
- Pathways
TRAX participates in the RNA regulatory network including the MAPK signaling pathway. It influences stress responses and cell survival by associating with proteins like Translin. This pathway coordination ensures cells can adapt to environmental changes efficiently important in maintaining neuronal health.
- Associated diseases and disorders
TRAX has links to neurodegenerative diseases and certain cancers. Alterations in TRAX expression or function can contribute to conditions such as schizophrenia. Its interaction with proteins in disease contexts including Translin suggests that misregulation of the TRAX-Translin complex may play a role in the pathology by impairing RNA transport and stability disrupting cellular functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532), expandable thumbnail](https://content.abcam.com/products/images/trax-antibody-epr13929b-bsa-and-azide-free-ab250532--immunoprecipitation-img74488.jpg "Immunoprecipitation - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532)")
Immunoprecipitation - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532)
This data was developed using Anti-TRAX antibody [EPR13929(B)] ab181862, the same antibody clone in a different buffer formulation.
Western blot analysis of immunoprecipitation pellet from Human testis lysate immunoprecipitated using Anti-TRAX antibody [EPR13929(B)] ab181862 at 1/60 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-TRAX antibody [EPR13929(B)] (Anti-TRAX antibody [EPR13929(B)] ab181862)
Predicted band size: 33 kDa
![Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532), expandable thumbnail](https://content.abcam.com/products/images/trax-antibody-epr13929b-bsa-and-azide-free-ab250532--western-blot-img58065.jpg "Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532)")
Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532)
This data was developed using Anti-TRAX antibody [EPR13929(B)] ab181862, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TRAX antibody [EPR13929(B)] (Anti-TRAX antibody [EPR13929(B)] ab181862) at 1/5000 dilution
All lanes: Human testis lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution
Predicted band size: 33 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532), expandable thumbnail](https://content.abcam.com/products/images/trax-antibody-epr13929b-bsa-and-azide-free-ab250532--immunocytochemistry-immunofluorescence-img62879.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532)")
Immunocytochemistry/ Immunofluorescence - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532)
This data was developed using Anti-TRAX antibody [EPR13929(B)] ab181862, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling TRAX with Anti-TRAX antibody [EPR13929(B)] ab181862 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532), expandable thumbnail](https://content.abcam.com/products/images/trax-antibody-epr13929b-bsa-and-azide-free-ab250532--western-blot-img61677.jpg "Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532)")
Western blot - Anti-TRAX antibody [EPR13929(B)] - BSA and Azide free (ab250532)
This data was developed using Anti-TRAX antibody [EPR13929(B)] ab181862, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TRAX antibody [EPR13929(B)] (Anti-TRAX antibody [EPR13929(B)] ab181862) at 1/20000 dilution
Lane 1: Mouse testis lysate at 20 µg
Lane 2: Rat testis lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 106 kDa, 24 kDa, 33 kDa, 52 kDa
Observed band size: 110 kDa, 25 kDa
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