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AB227699

Anti-TCR beta antibody [SP305]

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(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal T Cell Receptor antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

T cell receptor beta constant 1, TRBC1, TCR beta, TCRBC2, TRBC2, T cell receptor beta constant 2, TCR beta

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Formalin-fixed, paraffin-embedded human colon tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Formalin-fixed, paraffin-embedded human urinary bladder tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Formalin-fixed, paraffin-embedded human thymus tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Formalin-fixed, paraffin-embedded human stomach tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling TCR beta with ab227699 at 1/100 dilution (2.28 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling TCR beta with ab227699 at 1/100 dilution (2.28 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] (AB227699)

Formalin-fixed, paraffin-embedded human spleen stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-TCR beta antibody [SP305] (AB227699)
  • WB

Lab

Western blot - Anti-TCR beta antibody [SP305] (AB227699)

Blocking/Diluting buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-TCR beta antibody [SP305] (ab227699) at 0.228 µg/mL

Lane 1:

Ramos (Human Burkitt's lymphoma B lymphocyte) cell lysate

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) cell lysate

Lane 3:

HEK-293 ( Human embryonic kidney epithelial cell) cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 20 kDa

Observed band size: 35 kDa

false

Western blot - Anti-TCR beta antibody [SP305] (AB227699)
  • WB

Lab

Western blot - Anti-TCR beta antibody [SP305] (AB227699)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Exposure Time :
A. 40s
B. 7s
C. 7s

All lanes:

Western blot - Anti-TCR beta antibody [SP305] (ab227699) at 1/5000 dilution

Lane 1:

293T+OE-empty at 20 µg

Lanes 2 - 3:

293T+OE-TRBC1 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP305

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p>Primary antibody incubation for 1 hour at room temperature.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Primary antibody incubation for 10 minutes at room temperature.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRBC1 also known as T-Cell Receptor Beta Constant 1 is involved in the formation of the T-cell receptor complex. It has a molecular mass of around 18 kDa. This protein component is prominently expressed on the surface of T lymphocytes important for mediating immune responses. TRBC1 is a part of the TCR constant region playing a significant role in antigen recognition through its interaction with antigen-presenting cells.
Biological function summary

TRBC1 contributes to the immune system’s mechanism by helping identify foreign invaders in the body. It works as an integral part of the T-cell receptor (TCR) complex facilitating T-cell activation and signaling. The complex formation includes variable and constant regions with TRBC1 as a fixed part of the constant region ensuring the proper receptor structure for effective recognition. TCR assembly is important for T cells to detect specific antigens.

Pathways

TRBC1 participates in the adaptive immune response and T-cell receptor signaling pathway. Interacting proteins such as CD3 and ZAP70 help transmit signals initiated at the surface by antigen recognition leading to downstream effects such as cell proliferation and cytokine release. TRBC1’s involvement in these pathways ensures the T cells' ability to respond to invading pathogens and maintain immune surveillance.

TRBC1 has been implicated in conditions like autoimmune diseases and certain cancers such as T-cell lymphomas. Its interaction with proteins like CD3 impacts these disorders by amplifying or misdirecting immune responses. Aberrant TRBC1 expression or mutations can disrupt normal immune functions triggering disease development or progression. Understanding TRBC1's role in these contexts helps in developing targeted therapies and diagnostic tools.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of T cell receptor (TR) beta chain (PubMed : 24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed : 25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed : 23524462, PubMed : 9382891). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed : 15040585).
See full target information TRBC1

Additional targets

TRBC2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101852 PubMed39657664

2024

Lurbinectedin sensitizes PD-L1 blockade therapy by activating STING-IFN signaling in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Subhamoy Chakraborty,Utsav Sen,Kedwin Ventura,Vrinda Jethalia,Charles Coleman,Subhasree Sridhar,Avisek Banerjee,Hilal Ozakinci,Yazhini Mahendravarman,Konrad Snioch,Elisa de Stanchina,Misty D Shields,Lewis E Tomalin,Deniz Demircioglu,Theresa A Boyle,Anna Tocheva,Dan Hasson,Triparna Sen
View all publications
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Product promise

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