Rabbit Recombinant Monoclonal TRC40 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunoprecipitation - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--immunoprecipitation-img150971.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491)")
![Western blot - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--western-blot-img116740.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126522.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126722.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491)")
![Immunocytochemistry/ Immunofluorescence - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--immunocytochemistry-immunofluorescence-img10260.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (AB249491)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling.
Alternative names
ARSA, ASNA1, TRC40, GET3, ATPase GET3, Arsenical pump-driving ATPase, Arsenite-stimulated ATPase, Transmembrane domain recognition complex 40 kDa ATPase subunit, hARSA-I, hASNA-I
Recommended products
Rabbit Recombinant Monoclonal TRC40 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11423
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249491 is the carrier-free version of Anti-TRC40 antibody [EPR11423] ab169539.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRC40 also known as the transmembrane domain recognition complex subunit is a 41 kDa protein important for targeting and insertion of tail-anchored proteins into the endoplasmic reticulum membrane. It is well-expressed in human tissue especially those with high secretory or membrane protein activity. The protein interacts closely with the cytosolic components and serves as an important part of the guiding mechanism for specific proteins important for cellular function.
- Biological function summary
The targeting process involves TRC40 interacting with several tail-anchored proteins. It forms part of a delivery complex required for efficient insertion of these proteins into the correct cellular locations. TRC40 ensures accurate membrane localization firmly supporting cellular physiology and protein homeostasis. This activity is essential for maintaining the protein synthesis balance within the endoplasmic reticulum influencing many cellular metabolic processes.
- Pathways
TRC40 operates in the endoplasmic reticulum-associated degradation (ERAD) and secretory pathways. It cooperates with proteins like BAG6 and the Get3 complex acting as routing machinery for tail-anchored protein targeting. These pathways play a critical role in maintaining cellular protein composition by sorting membrane proteins essential for cellular health and stress responses.
- Associated diseases and disorders
TRC40 shows a significant role in neurodegenerative diseases and metabolic syndromes. Misregulation or dysfunction of its targeting process can disrupt protein homeostasis linking it to disorders such as Alzheimer's disease. The ERAD pathway involving TRC40 interacts with proteins like APP and presenilin contributing to the progression of these neurodegenerative conditions. Insights into TRC40's function reveal potential therapeutic targets in tackling related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--immunoprecipitation-img150971.jpg "Immunoprecipitation - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)")
Immunoprecipitation - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)
This data was developed using Anti-TRC40 antibody [EPR11423] ab169539, the same antibody clone in a different buffer formulation.Immunoprecipitation. Anti-TRC40 antibody [EPR11423] ab169539 at 1/10000 staining TRC40 in 293T cell lysate immunoprecipitated using Anti-TRC40 antibody [EPR11423] ab169539 at 1/10. Predicted band size : 39 kDa.
All lanes: Immunoprecipitation - Anti-TRC40 antibody [EPR11423] (Anti-TRC40 antibody [EPR11423] ab169539)
Predicted band size: 39 kDa
![Western blot - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--western-blot-img116740.jpg "Western blot - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)")
Western blot - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)
This data was developed using Anti-TRC40 antibody [EPR11423] ab169539, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TRC40 antibody [EPR11423] (Anti-TRC40 antibody [EPR11423] ab169539) at 1/10000 dilution
Lane 1: Fetal brain lysate at 10 µg
Lane 2: Fetal kidney lysate at 10 µg
Lane 3: HeLa lysate at 10 µg
Lane 4: 293T lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126522.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)
This data was developed using Anti-TRC40 antibody [EPR11423] ab169539, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TRC40 with Anti-TRC40 antibody [EPR11423] ab169539 antibody at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126722.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)
This data was developed using Anti-TRC40 antibody [EPR11423] ab169539, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human lung tissue labeling TRC40 with Anti-TRC40 antibody [EPR11423] ab169539 antibody at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--immunocytochemistry-immunofluorescence-img10260.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)")
Immunocytochemistry/ Immunofluorescence - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)
This data was developed using Anti-TRC40 antibody [EPR11423] ab169539, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling TRC40 with Anti-TRC40 antibody [EPR11423] ab169539 at 1/250.
![Flow Cytometry (Intracellular) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--flow-cytometry-intracellular-img37692.jpg "Flow Cytometry (Intracellular) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)")
Flow Cytometry (Intracellular) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)
This data was developed using Anti-TRC40 antibody [EPR11423] ab169539, the same antibody clone in a different buffer formulation.
Intracellular flow cytometrical analysis of permeabilized 293T cells with Anti-TRC40 antibody [EPR11423] ab169539 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
![Flow Cytometry (Intracellular) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491), expandable thumbnail](https://content.abcam.com/products/images/trc40-antibody-epr11423-bsa-and-azide-free-ab249491--flow-cytometry-intracellular-img37692.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TRC40 antibody [EPR11423] - BSA and Azide free (ab249491)")
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