AB272927

Anti-Trefoil Factor 3 antibody

Name: Anti-Trefoil Factor 3 antibody (ab272927) | Abcam
Brand: Abcam Limited
SKU: AB272927
Price: 530 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Trefoil Factor 3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TFF3.

View Alternative Names

ITF, TFI, TFF3, Trefoil factor 3, Intestinal trefoil factor, Polypeptide P1.B, hITF, hP1.B

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody (AB272927)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody (AB272927)

Paraffin-embedded mouse colon tissue stained for Trefoil Factor 3 using ab272927 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody (AB272927)

Paraffin-embedded mouse intestine tissue stained for Trefoil Factor 3 using ab272927 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TFF3. The exact immunogen used to generate this antibody is proprietary information.

Q07654

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trefoil Factor 3 (TFF3) also known as intestinal trefoil factor is a small peptide with a molecular mass around 7 kDa. This protein is characterized by the presence of a trefoil domain which consists of a stable compact structure important for its function. TFF3 has significant expression in the gastrointestinal tract notably in the colon and small intestine where it acts to maintain the integrity of the mucosal barrier. It can also be found in other tissues but its concentrations vary depending on factors like tissue type and health status.

Biological function summary

TFF3 plays an important role in protecting and repairing epithelial tissues particularly those lining the gastrointestinal tract. This protein promotes epithelial restitution by aiding cell migration and inhibits apoptosis contributing to tissue healing processes. While TFF3 does not form part of a large protein complex it often interacts with mucins specifically mucin 2 to bolster the protective mucus layer which is essential for shielding epithelial cells.

Pathways

Trefoil Factor 3 is notably involved in pathways associated with mucosal immune response and inflammation. A significant pathway involving TFF3 is the mucosal defense pathway where it works alongside other mucins and proteins like epidermal growth factor (EGF) to mediate regeneration and defense of epithelial tissues after injury. It also engages in the Wnt signaling pathway where it affects cell proliferation and migration critical for maintaining epithelial homeostasis.

TFF3 has links to inflammatory bowel disease (IBD) and colorectal cancer. In IBD its expression levels may be altered affecting the ability of the intestine to protect itself and heal contributing to the disease's progression. In colorectal cancer TFF3 can exhibit upregulated expression linking it to tumor growth and metastasis. Proteins such as beta-catenin in the Wnt signaling pathway which are associated with cell cycle regulation have connections with TFF3 highlighting its role in cancerous transformations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

See full target information TFF3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:10340 PubMed38710764

2024

Trefoil factor 3 can stimulate Th17 cell response in the development of type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyang Lin,Jinyuan Zhang,Tingting Duan,Junzheng Yang,Yiqi Yang

Endocrine 84:109-118 PubMed38148440

2023

Intestinal Trefoil Factor 3: a new biological factor mediating gut-kidney crosstalk in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Yinghui Zhang,Jie Tao,Xianglu Rong,Yiqi Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Trefoil Factor 3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody (AB272927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody (AB272927)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise