Anti-Trefoil Factor 3 antibody [EPR26048-14] - BSA and Azide free

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Trefoil Factor 3 antibody [EPR26048-14] - BSA and Azide free (AB300428)

This data was developed using ab300427, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labelling Eph receptor B2 with ab252935 at 1/500 dilution (B), GSDMC2 + GSDMC3 with ab229896 at 1/500 dilution (C) and Trefoil Factor 3 with ab300427 at 1/500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Eph receptor B2 (green; Opal™520), anti-GSDMC2/3 (magenta; Opal™690) and anti-Trefoil Factor 3 (gray; Opal™570) on mouse colon.

Panel B : anti-Eph receptor B2 staining stem cells in mouse colon.

Panel C : anti-GSDMC2/3 staining epithelium in mouse colon.

Panel D : anti-Trefoil Factor 3 staining goblet cells in mouse colon.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab252935, ab229896 and ab300427 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] - BSA and Azide free (AB300428)

This data was developed using ab300427, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Trefoil factor 3 with ab300427 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on mouse liver. The section was incubated with ab300427 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] - BSA and Azide free (AB300428)

This data was developed using ab300427, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon (fresh) tissue labeling Trefoil factor 3 with ab300427 at 1/500 (1.084 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on goblet cells of mouse colon is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. .

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] - BSA and Azide free (AB300428)

This data was developed using ab300427, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat colon (fresh) tissue labeling Trefoil factor 3 with ab300427 at 1/500 (1.084 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on goblet cells of rat colon is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. .

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] - BSA and Azide free (AB300428)

This data was developed using ab300427, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Trefoil factor 3 with ab300427 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on goblet cells of rat colon. The section was incubated with ab300427 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR26048-14] - BSA and Azide free (AB300428)

This data was developed using ab300427, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Trefoil factor 3 with ab300427 at 1/5000 (0.108 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on goblet cells of mouse colon. The section was incubated with ab300427 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] - BSA and Azide free (AB300428)

This data was developed using ab300427, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver (PMID : 7741746)

The identity of the band at approximately 37 kDa (in lane 2) is unknown.

All lanes:

Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (<a href='/en-us/products/primary-antibodies/trefoil-factor-3-antibody-epr26048-14-ab300427'>ab300427</a>) at 1/1000 dilution

Lane 1:

Mouse small intestine tissue lysate at 50 µg

Lane 2:

Mouse liver tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 8 kDa

Observed band size: 9 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] - BSA and Azide free (AB300428)

This data was developed using ab300427, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Trefoil Factor 3 antibody [EPR26048-14] (<a href='/en-us/products/primary-antibodies/trefoil-factor-3-antibody-epr26048-14-ab300427'>ab300427</a>) at 1/1000 dilution

All lanes:

Mouse colon tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 8 kDa

Observed band size: 9 kDa

false

Exposure time: 180s