Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic adenocarcinoma tissue labelling Trefoil Factor 3 with unpurified ab108599 at a dilution of 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muslce tissue labelling Trefoil Factor 3 with unpurified ab108599. Negative staining is shown.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human brain tissue labelling Trefoil Factor 3 with unpurified ab108599. Negative staining is shown.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Trefoil Factor 3 with unpurified ab108599 at a dilution of 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human uterus tissue labelling Trefoil Factor 3 with unpurified ab108599. Negative staining is shown.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Trefoil Factor 3 with purified ab108599 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial carcinoma tissue labelling Trefoil Factor 3 with unpurified ab108599.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human papillary carcinoma of thyroid gland tissue labelling Trefoil Factor 3 with unpurified ab108599.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Trefoil Factor 3 with unpurified ab108599.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic carcinoma tissue labelling Trefoil Factor 3 with purified ab108599 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

• IP

Lab

Immunoprecipitation - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

ab108599 (purified) at 1/30 immunoprecipitating Trefoil Factor 3 in human small intestine tissue lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

All lanes:

Immunoprecipitation - Anti-Trefoil Factor 3 antibody [EPR3974] (<a href='/en-us/products/primary-antibodies/trefoil-factor-3-antibody-epr3974-ab108599'>ab108599</a>)

Predicted band size: 8 kDa

false

• WB

Unknown

Western blot - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (ab239935) at 1/1000 dilution

All lanes:

Human colon tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 8 kDa

Observed band size: 9 kDa

false

• WB

Unknown

Western blot - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (ab239935) at 1/5000 dilution

All lanes:

Human small intestine tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 8 kDa

Observed band size: 9 kDa

false

• WB

Unknown

Western blot - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108599).

All lanes:

Western blot - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (ab239935) at 1/1000 dilution

Lane 1:

Human small intestine tissue lysate at 10 µg

Lane 2:

Human uterus tissue lysate at 10 µg

Predicted band size: 8 kDa

false

• WB

Lab

Western blot - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

False colour image of Western blot : Anti-Trefoil Factor 3 antibody [EPR3974] staining at 1/1000 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108599 was shown to bind specifically to Trefoil Factor 3. A band was observed at 10 kDa in wild-type MCF7 cell lysates with no signal observed at this size in TFF3 knockout cell line ab274936 (knockout cell lysate ab274994). To generate this image, wild-type and TFF3 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Trefoil Factor 3 antibody [EPR3974] (<a href='/en-us/products/primary-antibodies/trefoil-factor-3-antibody-epr3974-ab108599'>ab108599</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

TFF3 knockout MCF7 cell lysate at 20 µg

Lane 2:

Western blot - Human TFF3 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-tff3-knockout-mcf7-cell-line-ab274936'>ab274936</a>)

Lane 3:

Human colon cell lysate at 20 µg

Lane 4:

THP-1 cell lysate at 20 µg

Predicted band size: 8 kDa

Observed band size: 10 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Trefoil Factor 3 antibody [EPR3974] - BSA and Azide free (AB239935)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.